scholarly journals Functional and structural characterization of a novel putative cysteine protease cell wall-modifying multi-domain enzyme selected from a microbial metagenome

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Muhammad Faheem ◽  
Diogo Martins-de-Sa ◽  
Julia F. D. Vidal ◽  
Alice C. M. Álvares ◽  
José Brandão-Neto ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Cysteine Protease

Structural Characterization of a Highly Stable Cysteine Protease Ervatamin C

1999 ◽  
Vol 264 (3) ◽  
pp. 635-642 ◽  
Author(s):  
Suman Kundu ◽  
Monica Sundd ◽  
Medicherla V. Jagannadham
Keyword(s):  
Structural Characterization ◽  
Cysteine Protease

scholarly journals First Structural Characterization of a Bon-Domain in a Protein from Mycobacterium Tuberculosis: OmpATb Tracks toward an Oligomerization Process to form a Cell Wall Pore

2010 ◽  
Vol 98 (3) ◽  
pp. 648a
Author(s):  
Daniel Auguin ◽  
Yinshan Yang ◽  
Stephane Delbecq ◽  
Emilie Dumas ◽  
Virginie Molle ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Structural Characterization ◽  
A Cell

New Preparations of Lignin Polymer Models under Conditions that Approximate Cell Wall Lignification. II. Structural Characterization of the Models by Thioacidolysis

Holzforschung ◽  
1996 ◽  
Vol 50 (1) ◽  
pp. 9-14 ◽  
Author(s):  
N. Terashima ◽  
R.H. Atalla ◽  
S.A. Ralph ◽  
L.L. Landucci ◽  
C. Lapierre ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Polymer Models

Structural characterization of a cell wall polysaccharide from Penicillium vermoesenii: chemotaxonomic application

1999 ◽  
Vol 77 (7) ◽  
pp. 961-968 ◽  
Author(s):  
Oussama Ahrazem ◽  
Begoña Gómez-Miranda ◽  
Alicia Prieto ◽  
Isabel Barasoaín ◽  
Manuel Bernabé ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Cell Wall Polysaccharide ◽  
A Cell

Structural characterization of cell wall polysaccharides from two plant species endemic to central Africa, Fleurya aestuans and Phragmenthera capitata

2009 ◽  
Vol 75 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Sophie Aboughe Angone ◽  
Muriel Bardor ◽  
Eric Nguema-Ona ◽  
Christophe Rihouey ◽  
Tadashi Ishii ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Species ◽  
Structural Characterization ◽  
Central Africa ◽  
Cell Wall Polysaccharides

scholarly journals Characterization of O-Acetylation of N-Acetylglucosamine

2011 ◽  
Vol 286 (27) ◽  
pp. 23950-23958 ◽  
Author(s):  
Elvis Bernard ◽  
Thomas Rolain ◽  
Pascal Courtin ◽  
Alain Guillot ◽  
Philippe Langella ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Bacterial Species ◽  
Muramic Acid ◽  
Gram Positive Bacteria ◽  
Mutant Strains ◽  
Encoding Genes ◽  
Hydrolysis Of ◽  
Cell Wall Hydrolysis

Peptidoglycan (PG) N-acetyl muramic acid (MurNAc) O-acetylation is widely spread in Gram-positive bacteria and is generally associated with resistance against lysozyme and endogenous autolysins. We report here the presence of O-acetylation on N-acetylglucosamine (GlcNAc) in Lactobacillus plantarum PG. This modification of glycan strands was never described in bacteria. Fine structural characterization of acetylated muropeptides released from L. plantarum PG demonstrated that both MurNAc and GlcNAc are O-acetylated in this species. These two PG post-modifications rely on two dedicated O-acetyltransferase encoding genes, named oatA and oatB, respectively. By analyzing the resistance to cell wall hydrolysis of mutant strains, we showed that GlcNAc O-acetylation inhibits N-acetylglucosaminidase Acm2, the major L. plantarum autolysin. In this bacterial species, inactivation of oatA, encoding MurNAc O-acetyltransferase, resulted in marked sensitivity to lysozyme. Moreover, MurNAc over-O-acetylation was shown to activate autolysis through the putative N-acetylmuramoyl-l-alanine amidase LytH enzyme. Our data indicate that in L. plantarum, two different O-acetyltransferases play original and antagonistic roles in the modulation of the activity of endogenous autolysins.

scholarly journals Partial structural characterization of pectin cell wall from Argania spinosa leaves

Heliyon ◽  
2016 ◽  
Vol 2 (2) ◽  
pp. e00076 ◽  
Author(s):  
Kadda Hachem ◽  
Yasmina Benabdesslem ◽  
Samia Ghomari ◽  
Okkacha Hasnaoui ◽  
Meriem Kaid-Harche
Keyword(s):  
Cell Wall ◽  
Structural Characterization ◽  
Argania Spinosa
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