scholarly journals Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Qi-Lin Zhang ◽  
Qian-Hua Zhu ◽  
Xin Liao ◽  
Xiu-Qiang Wang ◽  
Tao Chen ◽  
...  
Keyword(s):  
Reference Genes ◽  
Developmental Stages ◽  
Rt Pcr ◽  
Selection Of

scholarly journals Spatio-temporal selection of reference genes in the two congeneric species of Glycyrrhiza

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuping Li ◽  
Xiaoju Liang ◽  
Xuguo Zhou ◽  
Yu An ◽  
Ming Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Developmental Stage ◽  
Reference Genes ◽  
Developmental Stages ◽  
Individual Factors ◽  
Congeneric Species ◽  
Spatio Temporal ◽  
The Stability ◽  
Different Tissues ◽  
Selection Of

AbstractGlycyrrhiza, a genus of perennial medicinal herbs, has been traditionally used to treat human diseases, including respiratory disorders. Functional analysis of genes involved in the synthesis, accumulation, and degradation of bioactive compounds in these medicinal plants requires accurate measurement of their expression profiles. Reverse transcription quantitative real-time PCR (RT-qPCR) is a primary tool, which requires stably expressed reference genes to serve as the internal references to normalize the target gene expression. In this study, the stability of 14 candidate reference genes from the two congeneric species G. uralensis and G. inflata, including ACT, CAC, CYP, DNAJ, DREB, EF1, RAN, TIF1, TUB, UBC2, ABCC2, COPS3, CS, R3HDM2, were evaluated across different tissues and throughout various developmental stages. More importantly, we investigated the impact of interactions between tissue and developmental stage on the performance of candidate reference genes. Four algorithms, including geNorm, NormFinder, BestKeeper, and Delta Ct, were used to analyze the expression stability and RefFinder, a comprehensive software, provided the final recommendation. Based on previous research and our preliminary data, we hypothesized that internal references for spatio-temporal gene expression are different from the reference genes suited for individual factors. In G. uralensis, the top three most stable reference genes across different tissues were R3HDM2, CAC and TUB, while CAC, CYP and ABCC2 were most suited for different developmental stages. CAC is the only candidate recommended for both biotic factors, which is reflected in the stability ranking for the spatio (tissue)-temporal (developmental stage) interactions (CAC, R3HDM2 and DNAJ). Similarly, in G. inflata, COPS3, R3HDM2 and DREB were selected for tissues, while RAN, COPS3 and CS were recommended for developmental stages. For the tissue-developmental stage interactions, COPS3, DREB and ABCC2 were the most suited reference genes. In both species, only one of the top three candidates was shared between the individual factors and their interactions, specifically, CAC in G. uralensis and COPS3 in G. inflata, which supports our overarching hypothesis. In summary, spatio-temporal selection of reference genes not only lays the foundation for functional genomics research in Glycyrrhiza, but also facilitates these traditional medicinal herbs to reach/maximize their pharmaceutical potential.

Selection of reference genes for real-time RT-PCR normalization in brown alga Undaria pinnatifida

2018 ◽  
Vol 31 (1) ◽  
pp. 787-793 ◽  
Author(s):  
Jing Li ◽  
Haishu Huang ◽  
Tifeng Shan ◽  
Shaojun Pang
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Brown Alga ◽  
Undaria Pinnatifida ◽  
Rt Pcr ◽  
Selection Of

scholarly journals Selection of optimal reference genes for normalization in quantitative RT-PCR

2010 ◽  
Vol 11 (1) ◽  
Author(s):  
Inna Chervoneva ◽  
Yanyan Li ◽  
Stephanie Schulz ◽  
Sean Croker ◽  
Chantell Wilson ◽  
...  
Keyword(s):  
Reference Genes ◽  
Rt Pcr ◽  
Selection Of

scholarly journals Corrigendum to “Quantitative Evaluation and Selection of Reference Genes for Quantitative RT-PCR in Mouse Acute Pancreatitis”

2017 ◽  
Vol 2017 ◽  
pp. 1-1
Author(s):  
Zhaoping Yan ◽  
Jinhang Gao ◽  
Xiuhe Lv ◽  
Wenjuan Yang ◽  
Shilei Wen ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Quantitative Evaluation ◽  
Reference Genes ◽  
Rt Pcr ◽  
Selection Of

scholarly journals Selection of reference genes for quantitative RT-PCR (RT-qPCR) analysis of rat tissues under physiological and toxicological conditions

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e855 ◽  
Author(s):  
Terje Svingen ◽  
Heidi Letting ◽  
Niels Hadrup ◽  
Ulla Hass ◽  
Anne Marie Vinggaard
Keyword(s):  
Reference Genes ◽  
Rat Tissues ◽  
Rt Pcr ◽  
Qpcr Analysis ◽  
Selection Of

Characterization and Selection of Reference Genes for Real-time Quantitative RT-PCR of Plants

2013 ◽  
Vol 47 (4) ◽  
pp. 427-436 ◽  
Author(s):  
Yuan Wei ◽  
Wan Hongjian ◽  
Yang Yuejian
Keyword(s):  
Real Time ◽  
Reference Genes ◽  
Rt Pcr ◽  
Selection Of
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