scholarly journals Bright blue-shifted fluorescent proteins with Cys in the GAF domain engineered from bacterial phytochromes: fluorescence mechanisms and excited-state dynamics

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Daria M. Shcherbakova ◽  
Mikhail Baloban ◽  
Jingyi Zhu ◽  
Vladislav V. Verkhusha ◽  
...  
2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jingyi Zhu ◽  
Daria M. Shcherbakova ◽  
Yusaku Hontani ◽  
Vladislav V. Verkhusha ◽  
John T. M. Kennis

2018 ◽  
Vol 207 ◽  
pp. 39-54 ◽  
Author(s):  
Shaina Dhamija ◽  
Bhisham Thakur ◽  
Purnananda Guptasarma ◽  
Arijit K. De

We present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual emission upon UV excitation in fluorescent proteins.


2019 ◽  
Author(s):  
Matthew M. Brister ◽  
Carlos Crespo-Hernández

<p></p><p> Damage to RNA from ultraviolet radiation induce chemical modifications to the nucleobases. Unraveling the excited states involved in these reactions is essential, but investigations aimed at understanding the electronic-energy relaxation pathways of the RNA nucleotide uridine 5’-monophosphate (UMP) have not received enough attention. In this Letter, the excited-state dynamics of UMP is investigated in aqueous solution. Excitation at 267 nm results in a trifurcation event that leads to the simultaneous population of the vibrationally-excited ground state, a longlived <sup>1</sup>n<sub>O</sub>π* state, and a receiver triplet state within 200 fs. The receiver state internally convert to the long-lived <sup>3</sup>ππ* state in an ultrafast time scale. The results elucidate the electronic relaxation pathways and clarify earlier transient absorption experiments performed for uracil derivatives in solution. This mechanistic information is important because long-lived nπ* and ππ* excited states of both singlet and triplet multiplicities are thought to lead to the formation of harmful photoproducts.</p><p></p>


2019 ◽  
Author(s):  
Gergely Samu ◽  
R.A. Scheidt ◽  
A. Balog ◽  
C. Janáky ◽  
P.V. Kamat

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