scholarly journals Seed sojourn and fast viability loss constrain seedling production of a prominent riparian protection plant Salix variegata Franch

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Qiaoli Ayi ◽  
Bo Zeng ◽  
Jianhui Liu ◽  
Shaohua Shi ◽  
Hangang Niu ◽  
...  
Keyword(s):  
Seedling Production ◽  
Viability Loss ◽  
Riparian Protection

Seedling Production of Cerrado Plant Species via Cutting for Degraded Area Recovery

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Evania Gondim ◽  
◽  
Josimara Nolasco ◽  
Franciliana Araujo Costa ◽  
◽  
...  
Keyword(s):  
Plant Species ◽  
Seedling Production ◽  
Degraded Area

scholarly journals Transcriptional CDK inhibitors, CYC065 and THZ1 promote Bim-dependent apoptosis in primary and recurrent GBM through cell cycle arrest and Mcl-1 downregulation

2021 ◽  
Vol 12 (8) ◽  
Author(s):  
Viktorija Juric ◽  
Lance Hudson ◽  
Joanna Fay ◽  
Cathy E. Richards ◽  
Hanne Jahns ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Cell Cycle Arrest ◽  
Cortical Neurons ◽  
Apoptotic Cell ◽  
Cdk Inhibitors ◽  
Induce Cell Death ◽  
Viability Loss ◽  
Cycle Arrest ◽  
Recurrent Gbm

AbstractActivation of cyclin-dependent kinases (CDKs) contributes to the uncontrolled proliferation of tumour cells. Genomic alterations that lead to the constitutive activation or overexpression of CDKs can support tumourigenesis including glioblastoma (GBM), the most common and aggressive primary brain tumour in adults. The incurability of GBM highlights the need to discover novel and more effective treatment options. Since CDKs 2, 7 and 9 were found to be overexpressed in GBM, we tested the therapeutic efficacy of two CDK inhibitors (CKIs) (CYC065 and THZ1) in a heterogeneous panel of GBM patient-derived cell lines (PDCLs) cultured as gliomaspheres, as preclinically relevant models. CYC065 and THZ1 treatments suppressed invasion and induced viability loss in the majority of gliomaspheres, irrespective of the mutational background of the GBM cases, but spared primary cortical neurons. Viability loss arose from G2/M cell cycle arrest following treatment and subsequent induction of apoptotic cell death. Treatment efficacies and treatment durations required to induce cell death were associated with proliferation velocities, and apoptosis induction correlated with complete abolishment of Mcl-1 expression, a cell cycle-regulated antiapoptotic Bcl-2 family member. GBM models generally appeared highly dependent on Mcl-1 expression for cell survival, as demonstrated by pharmacological Mcl-1 inhibition or depletion of Mcl-1 expression. Further analyses identified CKI-induced Mcl-1 loss as a prerequisite to establish conditions at which the BH3-only protein Bim can efficiently induce apoptosis, with cellular Bim amounts strongly correlating with treatment efficacy. CKIs reduced proliferation and promoted apoptosis also in chick embryo xenograft models of primary and recurrent GBM. Collectively, these studies highlight the potential of these novel CKIs to suppress growth and induce cell death of patient-derived GBM cultures in vitro and in vivo, warranting further clinical investigation.

Date Palm Wastes Co-composted Product: An Efficient Substrate for Tomato (Solanum lycopercicum L.) Seedling Production

2016 ◽  
Vol 9 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Wadii Abid ◽  
Salwa Magdich ◽  
Imen Ben Mahmoud ◽  
Khaled Medhioub ◽  
Emna Ammar
Keyword(s):  
Date Palm ◽  
Seedling Production

RFID-integrated Multi-Functional Remote Sensing System for Seedling Production Management

2008 ◽  
Author(s):  
I-Chang Yang ◽  
Suming Chen ◽  
Yu-I Huang ◽  
Kuang- Wen Hsieh ◽  
Chia-Tseng Chen ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Production Management ◽  
Seedling Production ◽  
Sensing System

scholarly journals Ganoderic Acid A Protects Rat H9c2 Cardiomyocytes from Hypoxia-Induced Injury via Up-Regulating miR-182-5p

2018 ◽  
Vol 50 (6) ◽  
pp. 2086-2096 ◽  
Author(s):  
Xiaohong  Zhang ◽  
Can Xiao ◽  
Hong Liu
Keyword(s):  
Cell Viability ◽  
Molecular Mechanisms ◽  
Akt Pathway ◽  
H9c2 Cell ◽  
H9c2 Cells ◽  
Ganoderic Acid ◽  
Expression Levels ◽  
Protein Levels ◽  
Viability Loss ◽  
H9c2 Cardiomyocytes

Background/Aims: Ganoderic acid A (GAA) isolated from Ganoderma lucidum, shows various benefit activities, such as anti-tumor activity, anti-HIV activity and hepatoprotective activity. However, the potential effects of GAA on hypoxia-induced injury of cardiomyocytes are still unclear. In this study, we aimed to reveal the effects of GAA on hypoxic-induced H9c2 cell injury, as well as potential underlying molecular mechanisms. Methods: Rat H9c2 cardiomyocytes were cultured in hypoxia condition with different doses of GAA. Cell viability and apoptosis were detected by CCK-8 assay and flow cytometry, respectively. qRT-PCR was performed to assess the expression levels of microRNA-182-5p (miR-182-5p) and phosphatase and tensin homologue (PTEN). Cell transfection was conducted to change the expression levels of miR-182-5p and PTEN in H9c2 cells. Finally, protein levels of key factors involved in cell proliferation, cell apoptosis and PTEN/PI3K/AKT pathway were evaluated using western blotting. Results: Hypoxia treatment significantly induced H9c2 cell viability loss and apoptosis. GAA incubation remarkably protected H9c2 cells from hypoxia-induced viability loss, proliferation inhibition and apoptosis. In addition, GAA obviously enhanced the expression level of miR-182-5p in H9c2 cells. Suppression of miR-182-5p notably alleviated the protective effects of GAA on hypoxia-treated H9c2 cells. Furthermore, miR-182-5p negatively regulated the mRNA and protein levels of PTEN in H9c2 cells. GAA attenuated hypoxia-induced inactivation of PI3K/AKT pathway in H9c2 cells by up-regulating miR-182-5p and then down-regulating PTEN. Conclusion: GAA protected rat H9c2 cardiomyocytes from hypoxia-induced injury might via up-regulating miR-182-5p, down-regulating PTEN and then activating PI3K/AKT signaling pathway.

Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis

1998 ◽  
Vol 8 (4) ◽  
pp. 463-471 ◽  
Author(s):  
N. W. Pammenter ◽  
Valerie Greggains ◽  
J. I. Kioko ◽  
J. Wesley-Smith ◽  
Patricia Berjak ◽  
...  
Keyword(s):  
Water Content ◽  
Exponential Function ◽  
Time Course ◽  
Drying Rate ◽  
Recalcitrant Seeds ◽  
Tissue Water ◽  
Initial Water ◽  
Viability Loss ◽  
Drying Rates ◽  
Water Contents

AbstractThe drying rate of whole seeds of Ekebergia capensis (Meliaceae) was shown to influence the response to desiccation, with rapidly dried seeds surviving to lower water contents. Short-term rapid drying (to water contents higher than those leading to viability loss) actually increased the rate of germination. The form of the time course of decline of axis water content varied with drying rate; slow drying could be described by an exponential function, whereas with rapid drying initial water loss was faster than predicted by an exponential function. These observations suggest that slow drying brought about homogeneous dehydration and that the rapid drying was uneven across the tissue. This raised the possibility that the different responses to dehydration were a function of different distributions of water in the axis tissue under the two drying regimes. However, ultrastructural observations indicated that different deleterious processes may be occurring under the different drying treatments. It was tentatively concluded that a major cause of viability loss in slowly dried material was likely to be a consequence of aqueous-based processes leading to considerable membrane degradation. Uneven distribution of tissue water could not be rejected as a contributory cause of the survival of rapidly dried seeds to low bulk water contents. The differential response to dehydration at different drying rates implies that it is not possible to determine a ‘critical water content’ for viability loss by recalcitrant seeds.

EVALUATION OF ORGANIC SOLID WASTES COMPOSTS AS PEAT SUBSTITUTES FOR SEEDLING PRODUCTION

2013 ◽  
Vol 36 (11) ◽  
pp. 1780-1794 ◽  
Author(s):  
Jian-Hua Zhang ◽  
Guang-Ming Tian ◽  
Gen-Di Zhou ◽  
Miao-Miao He ◽  
Fan Wang ◽  
...  
Keyword(s):  
Solid Wastes ◽  
Seedling Production ◽  
Organic Solid ◽  
Organic Solid Wastes

scholarly journals Effect of Supplementary Light Source on Quality of Grafted Tomato Seedlings and Expression of Two Photosynthetic Genes

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 207 ◽  
Author(s):  
Hao Wei ◽  
Jiangtao Hu ◽  
Chen Liu ◽  
Mengzhao Wang ◽  
Jin Zhao ◽  
...  
Keyword(s):  
Light Source ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Sources ◽  
Seedling Production ◽  
White Leds ◽  
Tomato Seedlings ◽  
Photosynthetic Genes ◽  
Supplementary Lighting

Supplementary lighting is commonly used in high-quality seedling production. In this study, grafted tomato seedlings were grown for 10 days in a glasshouse with 16-h daily supplementary lighting at 100 μmol·m−2·s−1 PPFD (Photosynthetic photon flux density) from either high-pressure sodium (HPS), metal halide (MH), far-red (FR), white LEDs (Light emitting diodes) (W), or mixed LEDs (W1R2B1, where the subscript numbers indicate the ratio of the LED chips) to determine which light sources improve the seedling quality. The control seedlings did not receive any supplementary light. Physiological parameters and the expression of genes related to photosynthesis were analyzed. The results showed that root length, biomass, number of leaves, chlorophyll (SPAD), scion dry weight to height ratio (WHR), and specific leaf weight (SLW) were the greatest for grafted seedlings grown in W1R2B1. The level of root ball formation was the greatest for seedlings grown in W1R2B1, followed by those grown in W, HPS, and MH. Seedlings grown in FR did not fare well, as they were very thin and weak. Moreover, the expression of two photosynthetic genes (PsaA and PsbA) was significantly increased by W1R2B1 and W, which suggests that the plastid or nuclear genes might be regulated. The overall results suggest that W1R2B1 was the most suitable light source to enhance the quality of grafted tomato seedlings. The results of this study could be used as a reference for seedling production in glasshouses, and may provide new insights in the research on lights affecting the development of plants.

scholarly journals Overcoming root dormancy and identifying the storage behaviour of Lilium polyphyllum seeds

Botany ◽  
2019 ◽  
Vol 97 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Anurag Dhyani ◽  
Carol C. Baskin ◽  
Bhagwati Prasad Nautiyal ◽  
Mohan Chandra Nautiyal
Keyword(s):  
Room Temperature ◽  
Seed Storage ◽  
Seedling Production ◽  
Spring Temperature ◽  
Indole Butyric Acid ◽  
Nitrogenous Compounds ◽  
Morphophysiological Dormancy ◽  
Mean Germination Time ◽  
The Mean ◽  
Endangered Medicinal Herb

Lilium polyphyllum D.Don ex Royle is a critically endangered medicinal herb of Himalaya. Seeds have epicotyl morphophysiological dormancy, and in the field a minimum of 34 weeks is required for root emergence. To date, treatments to decrease the time for root protrusion and seed storage behaviour have not been determined. We evaluated the effects of plant growth regulators and nitrogenous compounds as well as seed scarification on root emergence, and determined the storage behaviour of seeds. Root emergence at 20 °C was significantly increased by 100 ppm of indole butyric acid (IBA), KNO3, and thiourea, and by the application of NaOCl solution for 30 min. The mean germination time was significantly decreased by 50 ppm of gibberellic acid (GA3; 28 days) compared with 100 ppm GA3 (36 days). After 12 months of dry storage, the viability of seeds (TTC method) at room temperature (10–35 °C), 0–4 °C, and −20 ± 1 °C was 95%, 100%, and 100%, respectively; thus, the seeds have orthodox storage behaviour. Our recommendation for seedling production is to store the seeds at room temperature until they can be sown under warm wet conditions. Roots emerge after about 8 weeks, and then seeds should be given a 2-week cold moist treatment to break shoot dormancy. After 2 weeks of cold, transfer the seeds to warm (20 °C) spring temperature conditions to promote growth.

Sign in / Sign up

Export Citation Format

Share Document