Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification

Françoise Illien; Nicolas Rodriguez; Mehdi Amoura; Alain Joliot; Manjula Pallerla; Sophie Cribier; Fabienne Burlina; Sandrine Sagan

doi:10.1038/srep36938

Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification

Scientific Reports ◽

10.1038/srep36938 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 39

Author(s):

Françoise Illien ◽

Nicolas Rodriguez ◽

Mehdi Amoura ◽

Alain Joliot ◽

Manjula Pallerla ◽

...

Keyword(s):

Mass Spectrometry ◽

Flow Cytometry ◽

Fluorescence Spectroscopy ◽

Cell Penetrating Peptides ◽

Cell Penetrating ◽

Quantitative Fluorescence

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MALDI-TOF mass spectrometry: A powerful tool to study the internalization of cell-penetrating peptides

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/j.bbamem.2009.11.011 ◽

2010 ◽

Vol 1798 (12) ◽

pp. 2182-2189 ◽

Cited By ~ 22

Author(s):

Soline Aubry ◽

Baptiste Aussedat ◽

Diane Delaroche ◽

Chen-Yu Jiao ◽

Gérard Bolbach ◽

...

Keyword(s):

Mass Spectrometry ◽

Cell Penetrating Peptides ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Cell Penetrating

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Direct translocation of cell-penetrating peptides in liposomes: A combined mass spectrometry quantification and fluorescence detection study

Analytical Biochemistry ◽

10.1016/j.ab.2013.03.009 ◽

2013 ◽

Vol 438 (1) ◽

pp. 1-10 ◽

Cited By ~ 32

Author(s):

Astrid Walrant ◽

Lucrèce Matheron ◽

Sophie Cribier ◽

Stéphane Chaignepain ◽

Marie-Lise Jobin ◽

...

Keyword(s):

Mass Spectrometry ◽

Fluorescence Detection ◽

Cell Penetrating Peptides ◽

Cell Penetrating

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A direct approach to quantification of the cellular uptake of cell-penetrating peptides using MALDI-TOF mass spectrometry

Nature Protocols ◽

10.1038/nprot.2006.30 ◽

2006 ◽

Vol 1 (1) ◽

pp. 200-205 ◽

Cited By ~ 52

Author(s):

Fabienne Burlina ◽

Sandrine Sagan ◽

Gérard Bolbach ◽

Gérard Chassaing

Keyword(s):

Mass Spectrometry ◽

Cellular Uptake ◽

Cell Penetrating Peptides ◽

Direct Approach ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Cell Penetrating

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Quantification of the Cellular Uptake of Cell-Penetrating Peptides by MALDI-TOF Mass Spectrometry

Angewandte Chemie ◽

10.1002/ange.200500477 ◽

2005 ◽

Vol 117 (27) ◽

pp. 4316-4319 ◽

Cited By ~ 14

Author(s):

Fabienne Burlina ◽

Sandrine Sagan ◽

Gérard Bolbach ◽

Gérard Chassaing

Keyword(s):

Mass Spectrometry ◽

Cellular Uptake ◽

Cell Penetrating Peptides ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Cell Penetrating

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Quantification of the Cellular Uptake of Cell-Penetrating Peptides by MALDI-TOF Mass Spectrometry

Angewandte Chemie International Edition ◽

10.1002/anie.200500477 ◽

2005 ◽

Vol 44 (27) ◽

pp. 4244-4247 ◽

Cited By ~ 80

Author(s):

Fabienne Burlina ◽

Sandrine Sagan ◽

Gérard Bolbach ◽

Gérard Chassaing

Keyword(s):

Mass Spectrometry ◽

Cellular Uptake ◽

Cell Penetrating Peptides ◽

Maldi Tof Mass Spectrometry ◽

Maldi Tof ◽

Cell Penetrating

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Faculty Opinions recommendation of Tumor imaging by means of proteolytic activation of cell-penetrating peptides.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1022989.284802 ◽

2005 ◽

Author(s):

Wen-hong Li

Keyword(s):

Tumor Imaging ◽

Cell Penetrating Peptides ◽

Proteolytic Activation ◽

Cell Penetrating

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Faculty Opinions recommendation of Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.5869957.5858055 ◽

2011 ◽

Author(s):

Donald A Tomalia

Keyword(s):

Mr Imaging ◽

Cell Penetrating Peptides ◽

In Vivo Fluorescence ◽

Cell Penetrating

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Faculty Opinions recommendation of Cytotoxicity of prion protein-derived cell-penetrating peptides is modulated by pH but independent of amyloid formation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726937665.793537425 ◽

2017 ◽

Author(s):

Chandra Verma ◽

Suryani Lukman

Keyword(s):

Prion Protein ◽

Cell Penetrating Peptides ◽

Amyloid Formation ◽

Cell Penetrating

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Faculty Opinions recommendation of The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727683574.793533691 ◽

2017 ◽

Author(s):

Mario Lebendiker

Keyword(s):

Cell Penetrating Peptides ◽

Cell Penetrating ◽

One Step

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Antimicrobial and Cell-Penetrating Peptides: Structure, Assembly and Mechanisms of Membrane Lysis via Atomistic and Coarse-Grained Molecular Dynamic Simulations

Protein and Peptide Letters ◽

10.2174/0929866511009011313 ◽

2010 ◽

Vol 17 (11) ◽

pp. 1313-1327 ◽

Cited By ~ 30

Author(s):

Peter J. Bond ◽

Syma Khalid

Keyword(s):

Molecular Dynamic ◽

Cell Penetrating Peptides ◽

Coarse Grained ◽

Molecular Dynamic Simulations ◽

Dynamic Simulations ◽

Membrane Lysis ◽

Cell Penetrating ◽

Structure Assembly

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