scholarly journals Structural assemblies of the di- and oligomeric G-protein coupled receptor TGR5 in live cells: an MFIS-FRET and integrative modelling study

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Annemarie Greife ◽  
Suren Felekyan ◽  
Qijun Ma ◽  
Christoph G. W. Gertzen ◽  
Lina Spomer ◽  
...  
Keyword(s):  
G Protein ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Modelling Study

scholarly journals A pH-Sensitive Fluor, CypHerTM 5, Used to Monitor Agonist-Induced G Protein-Coupled Receptor Internalization in Live Cells

BioTechniques ◽  
2002 ◽  
Vol 33 (5) ◽  
pp. 1152-1157 ◽  
Author(s):  
E.J. Adie ◽  
S. Kalinka ◽  
L. Smith ◽  
M.J. Francis ◽  
A. Marenghi ◽  
...  
Keyword(s):  
G Protein ◽  
Ph Sensitive ◽  
Receptor Internalization ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals G protein–coupled receptor/arrestin3 modulation of the endocytic machinery

2002 ◽  
Vol 156 (4) ◽  
pp. 665-676 ◽  
Author(s):  
Francesca Santini ◽  
Ibragim Gaidarov ◽  
James H. Keen
Keyword(s):  
G Protein ◽  
Fluorescent Protein ◽  
De Novo ◽  
Membrane Skeleton ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
Cortical Actin ◽  
Endocytic Machinery ◽  
Green Fluorescent ◽  
G Protein Coupled

Nonvisual arrestins (arr) modulate G protein–coupled receptor (GPCR) desensitization and internalization and bind to both clathrin (CL) and AP-2 components of the endocytic coated pit (CP). This raises the possibility that endocytosis of some GPCRs may be a consequence of arr-induced de novo CP formation. To directly test this hypothesis, we examined the behavior of green fluorescent protein (GFP)-arr3 in live cells expressing β2-adrenergic receptors and fluorescent CL. After agonist stimulation, the diffuse GFP-arr3 signal rapidly became punctate and colocalized virtually completely with preexisting CP spots, demonstrating that activated complexes accumulate in previously formed CPs rather than nucleating new CP formation. After arr3 recruitment, CP appeared larger: electron microscopy analysis revealed an increase in both CP number and in the occurrence of clustered CPs. Mutant arr3 proteins with impaired binding to CL or AP-2 displayed reduced recruitment to CPs, but were still capable of inducing CP clustering. In contrast, though constitutively present in CPs, the COOH-terminal moiety of arr3, which contains CP binding sites but lacks receptor binding, did not induce CP clustering. Together, these results indicate that recruitment of functional arr3–GPCR complexes to CP is necessary to induce clustering. Latrunculin B or 16°C blocked CP rearrangements without affecting arr3 recruitment to CP. These results and earlier studies suggest that discrete CP zones exist on cell surfaces, each capable of supporting adjacent CPs, and that the cortical actin membrane skeleton is intimately involved with both the maintenance of existing CPs and the generation of new structures.

scholarly journals High Precision FRET Analysis of the G-Protein Coupled Receptor TGR5 in Live Cells

2014 ◽  
Vol 106 (2) ◽  
pp. 267a
Author(s):  
Annemarie Koch ◽  
Manuel Frohnapfel ◽  
Christoph Gertzen ◽  
Holger Gohlke ◽  
Verena Keitel ◽  
...  
Keyword(s):  
G Protein ◽  
High Precision ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells

2013 ◽  
Vol 288 (24) ◽  
pp. 17167-17178 ◽  
Author(s):  
Rabia U. Malik ◽  
Michael Ritt ◽  
Brian T. DeVree ◽  
Richard R. Neubig ◽  
Roger K. Sunahara ◽  
...  
Keyword(s):  
G Protein ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals High-precision FRET analysis of the G-protein coupled receptor TGR5 in live cells

2014 ◽  
Vol 19 (Suppl 1) ◽  
pp. S12
Author(s):  
Annemarie Koch ◽  
Qijun Ma ◽  
Manuel Frohnapfel ◽  
Lina Spomer ◽  
Verena Keitel-Anselmino ◽  
...  
Keyword(s):  
G Protein ◽  
High Precision ◽  
Live Cells ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Deconvolution of complex G protein–coupled receptor signaling in live cells using dynamic mass redistribution measurements

2010 ◽  
Vol 28 (9) ◽  
pp. 943-949 ◽  
Author(s):  
Ralf Schröder ◽  
Nicole Janssen ◽  
Johannes Schmidt ◽  
Anna Kebig ◽  
Nicole Merten ◽  
...  
Keyword(s):  
G Protein ◽  
Live Cells ◽  
Receptor Signaling ◽  
G Protein Coupled Receptor ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled
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