scholarly journals Opportunistic fungal pathogen Candida glabrata circulates between humans and yellow-legged gulls

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mohammed Hashim Al-Yasiri ◽  
Anne-Cécile Normand ◽  
Coralie L’Ollivier ◽  
Laurence Lachaud ◽  
Nathalie Bourgeois ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Opportunistic Fungal Pathogen

scholarly journals Multifactorial Role of Mitochondria in Echinocandin Tolerance Revealed by Transcriptome Analysis of Drug-Tolerant Cells

mBio ◽  
2021 ◽  
Author(s):  
Rocio Garcia-Rubio ◽  
Cristina Jimenez-Ortigosa ◽  
Lucius DeGregorio ◽  
Christopher Quinteros ◽  
Erika Shor ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Clinical Failure ◽  
First Line ◽  
Content Type ◽  
First Line Therapy ◽  
Line Therapy ◽  
Resistant Strains ◽  
Opportunistic Fungal Pathogen

Echinocandin drugs are a first-line therapy to treat invasive candidiasis, which is a major source of morbidity and mortality worldwide. The opportunistic fungal pathogen Candida glabrata is a prominent bloodstream fungal pathogen, and it is notable for rapidly developing echinocandin-resistant strains associated with clinical failure.

Candida glabrata: Multidrug Resistance and Increased Virulence in a Major Opportunistic Fungal Pathogen

2012 ◽  
Vol 6 (3) ◽  
pp. 154-164 ◽  
Author(s):  
Michael A. Pfaller ◽  
Mariana Castanheira ◽  
Shawn R. Lockhart ◽  
Ronald N. Jones
Keyword(s):  
Multidrug Resistance ◽  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Opportunistic Fungal Pathogen

scholarly journals yKu70/yKu80 and Rif1 Regulate Silencing Differentially at Telomeres in Candida glabrata

2008 ◽  
Vol 7 (12) ◽  
pp. 2168-2178 ◽  
Author(s):  
Lluvia L. Rosas-Hernández ◽  
Alejandro Juárez-Reyes ◽  
Omar E. Arroyo-Helguera ◽  
Alejandro De Las Peñas ◽  
Shih-Jung Pan ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Large Family ◽  
Reporter Genes ◽  
Cell Wall Proteins ◽  
Intergenic Regions ◽  
Reporter Strains ◽  
Opportunistic Fungal Pathogen ◽  
Subtelomeric Regions

ABSTRACT Candida glabrata, a common opportunistic fungal pathogen, adheres efficiently to mammalian epithelial cells in culture. This interaction in vitro depends mainly on the adhesin Epa1, one of a large family of cell wall proteins. Most of the EPA genes are located in subtelomeric regions, where they are transcriptionally repressed by silencing. In order to better characterize the transcriptional regulation of the EPA family, we have assessed the importance of C. glabrata orthologues of known regulators of subtelomeric silencing in Saccharomyces cerevisiae. To this end, we used a series of strains containing insertions of the reporter URA3 gene within different intergenic regions throughout four telomeres of C. glabrata. Using these reporter strains, we have assessed the roles of SIR2, SIR3, SIR4, HDF1 (yKu70), HDF2 (yKu80), and RIF1 in mediating silencing at four C. glabrata telomeres. We found that, whereas the SIR proteins are absolutely required for silencing of the reporter genes and the native subtelomeric EPA genes, the Rif1 and the Ku proteins regulate silencing at only a subset of the analyzed telomeres. We also mapped a cis element adjacent to the EPA3 locus that can silence a reporter gene when placed at a distance of 31 kb from the telomere. Our data show that silencing of the C. glabrata telomeres varies from telomere to telomere. In addition, recruitment of silencing proteins to the subtelomeres is likely, for certain telomeres, to depend both on the telomeric repeats and on particular discrete silencing elements.

The oxidative stress response of the opportunistic fungal pathogen Candida glabrata

2014 ◽  
Vol 31 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Marcela Briones-Martin-Del-Campo ◽  
Emmanuel Orta-Zavalza ◽  
Jacqueline Juarez-Cepeda ◽  
Guadalupe Gutierrez-Escobedo ◽  
Israel Cañas-Villamar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Oxidative Stress Response ◽  
Opportunistic Fungal Pathogen

scholarly journals The High-Osmolarity Glycerol Response Pathway in the Human Fungal Pathogen Candida glabrata Strain ATCC 2001 Lacks a Signaling Branch That Operates in Baker's Yeast

2007 ◽  
Vol 6 (9) ◽  
pp. 1635-1645 ◽  
Author(s):  
Christa Gregori ◽  
Christoph Schüller ◽  
Andreas Roetzer ◽  
Tobias Schwarzmüller ◽  
Gustav Ammerer ◽  
...  
Keyword(s):  
Map Kinase ◽  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Acid Tolerance ◽  
Weak Acid ◽  
Hog Pathway ◽  
High Osmolarity ◽  
High Osmolarity Glycerol ◽  
Mitogen Activated Protein ◽  
Opportunistic Fungal Pathogen

ABSTRACT The high-osmolarity glycerol (HOG) mitogen-activated protein (MAP) kinase pathway mediates adaptation to high-osmolarity stress in the yeast Saccharomyces cerevisiae. Here we investigate the function of HOG in the human opportunistic fungal pathogen Candida glabrata. C. glabrata sho1Δ (Cgsho1Δ) deletion strains from the sequenced ATCC 2001 strain display severe growth defects under hyperosmotic conditions, a phenotype not observed for yeast sho1Δ mutants. However, deletion of CgSHO1 in other genetic backgrounds fails to cause osmostress hypersensitivity, whereas cells lacking the downstream MAP kinase Pbs2 remain osmosensitive. Notably, ATCC 2001 Cgsho1Δ cells also display methylglyoxal hypersensitivity, implying the inactivity of the Sln1 branch in ATCC 2001. Genomic sequencing of CgSSK2 in different C. glabrata backgrounds demonstrates that ATCC 2001 harbors a truncated and mutated Cgssk2-1 allele, the only orthologue of yeast SSK2/SSK22 genes. Thus, the osmophenotype of ATCC 2001 is caused by a point mutation in Cgssk2-1, which debilitates the second HOG pathway branch. Functional complementation experiments unequivocally demonstrate that HOG signaling in yeast and C. glabrata share similar functions in osmostress adaptation. In contrast to yeast, however, Cgsho1Δ mutants display hypersensitivity to weak organic acids such as sorbate and benzoate. Hence, CgSho1 is also implicated in modulating weak acid tolerance, suggesting that HOG signaling in C. glabrata mediates the response to multiple stress conditions.

The EPA2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen Candida glabrata

2015 ◽  
Vol 61 (4) ◽  
pp. 529-544 ◽  
Author(s):  
Jacqueline Juárez-Cepeda ◽  
Emmanuel Orta-Zavalza ◽  
Israel Cañas-Villamar ◽  
Jorge Arreola-Gómez ◽  
Gloria Patricia Pérez-Cornejo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Encoding Gene ◽  
Opportunistic Fungal Pathogen

scholarly journals Subtelomeric Silencing of the MTL3 Locus of Candida glabrata Requires yKu70, yKu80, and Rif1 Proteins

2010 ◽  
Vol 9 (10) ◽  
pp. 1602-1611 ◽  
Author(s):  
Candy Y. Ramírez-Zavaleta ◽  
Griselda E. Salas-Delgado ◽  
Alejandro De Las Peñas ◽  
Irene Castaño
Keyword(s):  
Mating Type ◽  
Fungal Pathogen ◽  
Candida Glabrata ◽  
Sexual Cycle ◽  
Content Type ◽  
Internal Position ◽  
Cell Type Specific ◽  
Opportunistic Fungal Pathogen ◽  
Reported Data ◽  
Type Information

ABSTRACT Candida glabrata is a haploid opportunistic fungal pathogen that is phylogenetically related to Saccharomyces cerevisiae. Even though C. glabrata has no known sexual cycle, it contains, like S. cerevisiae, three mating type-like loci (MTL) called MTL1, MTL2, and MTL3, as well as most of the genes required for mating, meiosis, and sporulation. MTL1 is localized at an internal position on chromosome B and is thought to be the locus corresponding to the MAT locus in S. cerevisiae. MTL2 and MTL3 are localized close to two telomeres on different chromosomes (29.4 kb from Chr E-L and 10.5 kb from Chr B-L, respectively). By using URA3 reporter gene insertions at the three MTL loci, we found that in contrast to the case for S. cerevisiae, only MTL3 is subject to transcriptional silencing while MTL2 is transcriptionally active, and this is in agreement with previously reported data. We found that the silencing of MTL3 is nucleated primarily at the left telomere of chromosome B and spreads over 12 kb to MTL3, rather than nucleating at flanking, closely positioned cis-acting silencers, like those flanking HMR and HML of S. cerevisiae. Interestingly, the silencing of MTL3 absolutely requires the yKu70, yKu80, and Rif1 proteins, in sharp contrast to the silencing of the HM loci of S. cerevisiae. In addition, we found that several cell type-specific genes are expressed in C. glabrata regardless of the presence, or even absence, of mating type information at any of the MTL loci.

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