scholarly journals Multilocus sequence analysis reveals high genetic diversity in clinical isolates of Burkholderia cepacia complex from India

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Vikas Gautam ◽  
Prashant P. Patil ◽  
Sunil Kumar ◽  
Samriti Midha ◽  
Mandeep Kaur ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sequence Analysis ◽  
Burkholderia Cepacia ◽  
Clinical Isolates ◽  
Burkholderia Cepacia Complex ◽  
Multilocus Sequence Analysis ◽  
High Genetic Diversity

scholarly journals Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections

2020 ◽  
Vol 9 (1) ◽  
pp. 51
Author(s):  
Min Yi Wong ◽  
Yuan-Hsi Tseng ◽  
Tsung-Yu Huang ◽  
Bor-Shyh Lin ◽  
Chun-Wu Tung ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vascular Access ◽  
Burkholderia Cepacia ◽  
Bacterial Species ◽  
Diversity Indices ◽  
Burkholderia Cepacia Complex ◽  
Reca Gene ◽  
Clinical Effects ◽  
Environmental Distribution ◽  
Microbiological Characteristics

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.

scholarly journals Swimming Motility in a Longitudinal Collection of Clinical Isolates of Burkholderia cepacia Complex Bacteria from People with Cystic Fibrosis

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e106428 ◽  
Author(s):  
James E. A. Zlosnik ◽  
Paul Y. Mori ◽  
Derek To ◽  
James Leung ◽  
Trevor J. Hird ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Burkholderia Cepacia ◽  
Clinical Isolates ◽  
Burkholderia Cepacia Complex ◽  
Swimming Motility

scholarly journals Diversity of cwp loci in clinical isolates of Clostridium difficile

2013 ◽  
Vol 62 (9) ◽  
pp. 1444-1452 ◽  
Author(s):  
Manuele Biazzo ◽  
Rossella Cioncada ◽  
Luigi Fiaschi ◽  
Vittorio Tedde ◽  
Patrizia Spigaglia ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Clostridium Difficile ◽  
Total Cell ◽  
The Other ◽  
Clinical Isolates ◽  
Cell Wall Proteins ◽  
High Genetic Diversity ◽  
Cell Extracts ◽  
Number Of Genes ◽  
Genes Encoding

An increased incidence of Clostridium difficile infection (CDI) is associated with the emergence of epidemic strains characterized by high genetic diversity. Among the factors that may have a role in CDI is a family of 29 paralogues, the cell-wall proteins (CWPs), which compose the outer layer of the bacterial cell and are likely to be involved in colonization. Previous studies have shown that 12 of the 29 cwp genes are clustered in the same region, named after slpA (cwp1), the slpA locus, whereas the remaining 17 paralogues are distributed throughout the genome. The variability of 14 of these 17 cwp paralogues was determined in 40 C. difficile clinical isolates belonging to six of the currently prevailing PCR ribotypes. Based on sequence conservation, these cwp genes were divided into two groups, one comprising nine cwp loci having highly conserved sequences in all isolates, and the other five loci showing low genetic conservation among isolates of the same PCR ribotype, as well as between different PCR ribotypes. Three conserved CWPs, Cwp16, Cwp18 and Cwp25, and two variable ones, Cwp26 and Cwp27, were characterized further by Western blot analysis of total cell extracts or surface-layer preparations of the C. difficile clinical isolates. Expression of genetically invariable CWPs was well conserved in all isolates, whilst genetically variable CWPs were not always expressed at comparable levels, even in strains containing identical sequences but belonging to different PCR ribotypes. This is the first report on the distribution and variability of a number of genes encoding CWPs in C. difficile.

scholarly journals Multilocus Sequence Analysis of Xanthomonads Causing Bacterial Spot of Tomato and Pepper Plants Reveals Strains Generated by Recombination among Species and Recent Global Spread of Xanthomonas gardneri

2014 ◽  
Vol 81 (4) ◽  
pp. 1520-1529 ◽  
Author(s):  
Sujan Timilsina ◽  
Mustafa O. Jibrin ◽  
Neha Potnis ◽  
Gerald V. Minsavage ◽  
Misrak Kebede ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sequence Analysis ◽  
Housekeeping Genes ◽  
Global Distribution ◽  
Multilocus Sequence Analysis ◽  
Regional Differentiation ◽  
Bacterial Spot ◽  
Content Type ◽  
Global Spread ◽  
Pathogen Populations

ABSTRACTFourXanthomonasspecies are known to cause bacterial spot of tomato and pepper, but the global distribution and genetic diversity of these species are not well understood. A collection of bacterial spot-causing strains from the Americas, Africa, Southeast Asia, and New Zealand were characterized for genetic diversity and phylogenetic relationships using multilocus sequence analysis of six housekeeping genes. By examining strains from different continents, we found unexpected phylogeographic patterns, including the global distribution of a single multilocus haplotype ofX. gardneri, possible regional differentiation inX. vesicatoria, and high species diversity on tomato in Africa. In addition, we found evidence of multiple recombination events betweenX. euvesicatoriaandX. perforans.Our results indicate that there have been shifts in the species composition of bacterial spot pathogen populations due to the global spread of dominant genotypes and that recombination between species has generated genetic diversity in these populations.

scholarly journals Genetic Diversity and Population Structure of Vibrio parahaemolyticus Isolated From Clinical and Food Sources

2021 ◽  
Vol 12 ◽  
Author(s):  
Min He ◽  
Tao Lei ◽  
Fufeng Jiang ◽  
Jumei Zhang ◽  
Haiyan Zeng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vibrio Parahaemolyticus ◽  
Large Scale ◽  
Foodborne Pathogen ◽  
Guangdong Province ◽  
Housekeeping Genes ◽  
Clinical Isolates ◽  
Food Sources ◽  
Clinical Samples ◽  
High Genetic Diversity

Vibrio parahaemolyticus is a common foodborne pathogen that causes gastroenteritis worldwide. Determining its prevalence and genetic diversity will minimize the risk of infection and the associated economic burden. Multilocus sequence typing (MLST) is an important tool for molecular epidemiology and population genetic studies of bacteria. Here, we analyzed the genetic and evolutionary relationships of 162 V. parahaemolyticus strains isolated in the Guangdong Province, China, using MLST. In the study, 120 strains were isolated from food samples, and 42 strains were isolated from clinical samples. All strains were categorized into 100 sequence types (STs), of which 58 were novel (48 from the food isolates and 10 from the clinical isolates). ST415 was the most prevalent ST among the food isolates, while ST3 was the most prevalent ST among the clinical isolates. Further, 12 clonal complexes, 14 doublets, and 73 singletons were identified in all ST clusters, indicating high genetic diversity of the analyzed strains. At the concatenated sequence level, non-synonymous sites in both, food and clinical isolates, were associated with purifying selection. Of note, the dN/dS ration was greater than 1 for some housekeeping genes in all isolates. This is the first time that some loci under positive selection were identified. These observations confirm frequent recombination events in V. parahaemolyticus. Recombination was much more important than mutation for genetic heterogeneity of the food isolates, but the probabilities of recombination and mutations were almost equal for the clinical isolates. Based on the phylogenetic analysis, the clinical isolates were concentrated in the maximum-likelihood tree, while the food isolates were heterogeneously distributed. In conclusion, the food and clinical isolates of V. parahaemolyticus from the Guangdong Province are similar, but show different evolutionary trends. This may help prevent large-scale spread of highly virulent strains and provides a genetic basis for the discovery of microevolutionary relationships in V. parahaemolyticus populations.

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