scholarly journals Caudal migration and proliferation of renal progenitors regulates early nephron segment size in zebrafish

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Richard W. Naylor ◽  
Rachel C. Dodd ◽  
Alan J. Davidson
Keyword(s):  
Segment Size ◽  
Nephron Segment ◽  
Renal Progenitors

scholarly journals Author response: Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development

2016 ◽  
Author(s):  
Shahram Jevin Poureetezadi ◽  
Christina N Cheng ◽  
Joseph M Chambers ◽  
Bridgette E Drummond ◽  
Rebecca A Wingert
Keyword(s):  
Kidney Development ◽  
Author Response ◽  
Nephron Segment ◽  
Renal Progenitors

scholarly journals Prostaglandin signaling regulates nephron segment patterning of renal progenitors during zebrafish kidney development

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Shahram Jevin Poureetezadi ◽  
Christina N Cheng ◽  
Joseph M Chambers ◽  
Bridgette E Drummond ◽  
Rebecca A Wingert
Keyword(s):  
Birth Defects ◽  
Kidney Development ◽  
Distal Segment ◽  
Genetic Screen ◽  
Prostaglandin Synthesis ◽  
Lipid Mediators ◽  
Chemical Genetic ◽  
Nephron Segment ◽  
Renal Progenitors ◽  
First Time

Kidney formation involves patterning events that induce renal progenitors to form nephrons with an intricate composition of multiple segments. Here, we performed a chemical genetic screen using zebrafish and discovered that prostaglandins, lipid mediators involved in many physiological functions, influenced pronephros segmentation. Modulating levels of prostaglandin E2 (PGE2) or PGB2 restricted distal segment formation and expanded a proximal segment lineage. Perturbation of prostaglandin synthesis by manipulating Cox1 or Cox2 activity altered distal segment formation and was rescued by exogenous PGE2. Disruption of the PGE2 receptors Ptger2a and Ptger4a similarly affected the distal segments. Further, changes in Cox activity or PGE2 levels affected expression of the transcription factors irx3b and sim1a that mitigate pronephros segment patterning. These findings show for the first time that PGE2 is a regulator of nephron formation in the zebrafish embryonic kidney, thus revealing that prostaglandin signaling may have implications for renal birth defects and other diseases.

scholarly journals Metanephric mesenchyme contains multipotent stem cells whose fate is restricted after induction

Development ◽  
1992 ◽  
Vol 114 (3) ◽  
pp. 565-572 ◽  
Author(s):  
D. Herzlinger ◽  
C. Koseki ◽  
T. Mikawa ◽  
Q. al-Awqati
Keyword(s):  
Stem Cells ◽  
Cell Types ◽  
Metanephric Mesenchyme ◽  
Multipotent Stem Cells ◽  
Nephron Segment ◽  
Single Nephron ◽  
Single Nephron Segment ◽  
Heterogenous Population ◽  
Renal Progenitors ◽  
Lac Z

At least fourteen epithelial cell types of the mammalian nephron develop from the metanephric mesenchyme. To distinguish whether this single embryological primordium contains a heterogenous population of committed renal cell lines or a multipotent stem cell, the lac-Z gene was introduced into individual renal progenitors by retroviral mediated gene transfer. The differentiated fate of lac-Z-tagged daughters derived from single metanephric mesenchymal cells was characterized after cytodifferentiation. We found that the metanephric mesenchyme contains multipotent stem cells that can generate at least three distinct cell types; glomerular, proximal and distal epithelia. After induction the fate of this multipotent cell becomes restricted to populate a single nephron segment.

Multihormonal regulation of nephron epithelia: achieved through combinational mode?

1995 ◽  
Vol 269 (4) ◽  
pp. R739-R748 ◽  
Author(s):  
C. De Rouffignac
Keyword(s):  
Sodium Chloride ◽  
Hormonal Regulation ◽  
Biological Effects ◽  
Basolateral Membrane ◽  
Positive Interactions ◽  
Thick Ascending Limb ◽  
Transport Pathways ◽  
Receptor Complexes ◽  
Nephron Segment ◽  
Urinary Concentrating Ability

The kidney is the main organ regulating composition of body fluids. A considerable number of hormones control the activity of renal cells to maintain hydromineral equilibrium. It becomes more and more difficult to interpret this multihormonal control in terms of regulatory processes. To illustrate this complexity, the hormonal regulation of electrolyte transport in the nephron thick ascending limb is taken as an example. This nephron segment is largely responsible for two kidney functions: the urinary-concentrating ability (by its capacity to deliver hypertonic sodium chloride into the medullary interstitium) and regulation of magnesium excretion into final urine. Six hormones are presently identified as acting on the transport of both sodium chloride and magnesium ions in this nephron segment. Therefore, the pertinent question is how the thick ascending limb and, hence, the kidney, is capable of regulating water balance independently from magnesium balance. It is proposed that the hormones act in combination: circulating levels of the individual hormones acting on these cells may determine the configuration of the paracellular and transcellular transport pathways of the epithelium either in the “sodium” or “magnesium” mode. The configuration would depend on the distribution and activity of the receptor at the surface of the basolateral membrane in contact with the circulating hormones. This distribution along with stimulation of respective signal transduction pathways would lead to the final biological effects. It is already known that the distribution of cell receptors may vary according to factors such as age, nutritional variability, hormonal status, degree of desensitization of the receptors, etc. The modulation of hormonal responses would depend therefore on the degree of coupling of hormone-receptor complexes to different intracellular transduction pathways and on the resulting negative and/or positive interactions between these pathways.

INDUCTION OF NEPHRONS FROM RENAL PROGENITORS BY MULTIPLE SIGNALS

ASAIO Journal ◽  
2003 ◽  
Vol 49 (2) ◽  
pp. 198
Author(s):  
J Barasch ◽  
J Yang ◽  
K Mori
Keyword(s):  
Multiple Signals ◽  
Renal Progenitors

Immunolocalization of NHE8 in rat kidney

2005 ◽  
Vol 288 (3) ◽  
pp. F530-F538 ◽  
Author(s):  
Sunita Goyal ◽  
SueAnn Mentone ◽  
Peter S. Aronson
Keyword(s):  
Proximal Tubule ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Rat Kidney ◽  
Surface Membrane ◽  
Proximal Tubules ◽  
Intense Staining ◽  
Proximal Tubule Cells ◽  
Nephron Segment ◽  
Tubule Cells

In situ hybridization studies demonstrated that Na+/H+ exchanger NHE8 is expressed in kidney proximal tubules. Although membrane fractionation studies suggested apical brush-border localization, precise membrane localization could not be definitively established. The goal of the present study was to develop isoform-specific NHE8 antibodies as a tool to directly establish the localization of NHE8 protein in the kidney by immunocytochemistry. Toward this goal, two sets of antibodies that label different NHE8 epitopes were developed. Monoclonal antibody 7A11 and polyclonal antibody Rab65 both specifically labeled NHE8 by Western blotting as well as by immunofluorescence microscopy. The immunolocalization pattern in the kidney seen with both antibodies was the same, thereby validating NHE8 specificity. In particular, NHE8 expression was observed on the apical brush-border membrane of all proximal tubules from S1 to S3. The most intense staining was evident in proximal tubules in the deeper cortex and medulla with a significant but somewhat weaker staining in superficial proximal tubules. Colocalization studies with γ-glutamyltranspeptidase and megalin indicated expression of NHE8 on both the microvillar surface membrane and the coated-pit region of proximal tubule cells, suggesting that NHE8 may be subject to endocytic retrieval and recycling. Although colocalizing in the proximal tubule with NHE3, no significant alteration in NHE8 protein expression was evident in NHE3-null mice. We conclude that NHE8 is expressed on the apical brush-border membrane of proximal tubule cells, where it may play a role in mediating or regulating ion transport in this nephron segment.

Nephron segment identification in the normal canine kidney by using lectin histochemistry

2012 ◽  
Vol 93 (2) ◽  
pp. 560-564 ◽  
Author(s):  
Akira Yabuki ◽  
Sawane Mitani ◽  
Keijiro Mizukami ◽  
Osamu Yamato
Keyword(s):  
Lectin Histochemistry ◽  
Nephron Segment ◽  
Canine Kidney
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