scholarly journals Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shohei Hayashi ◽  
Yasuharu Satoh ◽  
Tetsuro Ujihara ◽  
Yusuke Takata ◽  
Tohru Dairi
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Enzyme Engineering ◽  
Carrier Proteins ◽  
Acyl Carrier Proteins ◽  
Enhanced Production

scholarly journals Products of fatty acid synthesis by a particulate fraction from germinating pea (Pisum sativum L.)

1981 ◽  
Vol 199 (1) ◽  
pp. 221-226 ◽  
Author(s):  
J Sanchez ◽  
J L Harwood
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Pisum Sativum ◽  
Acyl Carrier Protein ◽  
Acid Synthesis ◽  
Simultaneous Increase ◽  
Carrier Protein ◽  
Carrier Proteins ◽  
Acyl Carrier Proteins ◽  
Malonyl Coa

The synthesis of lipids and acyl thioesters was studied in microsomal preparations from germinating pea (Pisum sativum cv. Feltham First) seeds. Under conditions of maximal synthesis (in the presence of exogenous acyl-carrier protein) acyl-acyl-carrier proteins accounted for about half the total incorporation from [14C]malonyl-CoA. Decreasing the concentrations of exogenous acyl-carrier protein lowered the overall synthesis of fatty acids by decreasing, almost exclusively, the radioactivity associated with acyl-acyl-carrier proteins. A time-course experiment showed that acyl-acyl-carrier proteins accumulated most of the radioactive label at the beginning of the incubation but, eventually, the amount of radioactivity in that fraction decreased, while a simultaneous increase in the acyl-CoA and lipid fractions was noticed. Addition of exogenous CoA (1 mM) produced a decrease of total incorporation, but an increase in the radioactivity incorporated into acyl-CoA. The microsomal preparations synthesized saturated fatty acids up to C20, including significant proportions of pentadecanoic acid and heptadecanoic acid. Synthesis of these ‘odd-chain’ fatty acids only took place in the microsomal fraction. In contrast, when the 18,000g supernatant (containing the microsomal and soluble fractions) was incubated with [14C]malonyl-CoA, the radioactive fatty acid and acyl classes closely resembled the patterns produced by germinating in the presence of [14C]acetate in vivo. The results are discussed in relation to the role of acyl thioesters in the biosynthesis of plant lipids.

scholarly journals Artificial covalent linkage of bacterial acyl carrier proteins for fatty acid production

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Carlos Rullán-Lind ◽  
Melissa Ortiz-Rosario ◽  
Andrea García-González ◽  
Vivian Stojanoff ◽  
Nataliya E. Chorna ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Tandem Repeats ◽  
High Fatty Acid ◽  
Size Exclusion ◽  
Carrier Proteins ◽  
Acyl Carrier Proteins ◽  
Fatty Acid Production ◽  
Acid Yield ◽  
Exclusion Chromatography

Abstract Acyl carrier proteins (ACPs) are essential to the production of fatty acids. In some species of marine bacteria, ACPs are arranged into tandem repeats joined by peptide linkers, an arrangement that results in high fatty acid yields. By contrast, Escherichia coli, a relatively low producer of fatty acids, uses a single-domain ACP. In this work, we have engineered the native E. coli ACP into tandem di- and tri-domain constructs joined by a naturally occurring peptide linker from the PUFA synthase of Photobacterium profundum. The size of these tandem fused ACPs was determined by size exclusion chromatography to be higher (21 kDa, 36 kDa and 141 kDa) than expected based on the amino acid sequence (12 kDa, 24 kDa and 37 kDa, respectively) suggesting the formation of a flexible extended conformation. Structural studies using small-angle X-ray scattering (SAXS), confirmed this conformational flexibility. The thermal stability for the di- and tri-domain constructs was similar to that of the unfused ACP, indicating a lack of interaction between domains. Lastly, E. coli cultures harboring tandem ACPs produced up to 1.6 times more fatty acids than wild-type ACP, demonstrating the viability of ACP fusion as a method to enhance fatty acid yield in bacteria.

Enhanced production of triacylglycerols and polyunsaturated fatty acids in novel acid-tolerant mutants of the green microalga Chlorella saccharophila

2019 ◽  
Vol 42 (10) ◽  
pp. 1561-1571
Author(s):  
Jalsen Iván Teco-Bravo ◽  
Luis Felipe Barahona-Pérez ◽  
Carlos Francisco Reyes-Sosa ◽  
Ángela Francisca Ku-González ◽  
Virginia Aurora Herrera-Valencia ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Enhanced Production ◽  
Chlorella Saccharophila ◽  
Green Microalga ◽  
Acid Tolerant

The possible involvement of a fatty acyl thioesterase in the termination of the synthesis of fatty acids in a psychrophilic bacterium, Vibrio sp. strain ABE-1

1993 ◽  
Vol 39 (10) ◽  
pp. 994-998
Author(s):  
Naoki Morita ◽  
Hidetoshi Okuyama
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acyl Chain ◽  
Fatty Acid Synthetase ◽  
Fatty Acyl ◽  
Carrier Proteins ◽  
Acyl Carrier Proteins ◽  
Cytosol Fraction ◽  
Acyl Chain Length

In Vibrio sp. strain ABE-1, very long chain fatty acids with up to 30 carbon atoms were synthesized in vitro in the form of acyl-acyl carrier protein by a fatty acid synthetase that had been prepared from the cytosol fraction precipitated between 55 and 75% saturation with ammonium sulfate. In contrast, fatty acids with 10–18 carbon atoms, which are the usual acyl components in this bacterium, were synthesized in vitro when the unfractionated cytosol fraction was used as the source of catalytic activity. When partially purified fatty acid synthetase was used together with a subfraction that had been prepared from the cytosol fraction precipitated between 0 and 55% saturation with ammonium sulfate, 16-carbon fatty acids were recovered as the dominant free fatty acids, and fatty acids with more than 20 carbon atoms were not synthesized in vitro. Acyl-acyl carrier proteins and acyl-CoAs with 16-carbon fatty acids were preferentially hydrolyzed when this subfraction was used as the source of catalytic activity. These results suggest that (a) fatty acyl thioesterase(s) with high specificity for acyl-acyl carrier proteins with 16-carbon fatty acids regulate(s) acyl chain length. This activity could explain the high levels of 16-carbon fatty acids in this bacterium.Key words: acyl chain length regulation, fatty acyl thioesterase, acyl-ACP, fatty acid synthetase, psychrophilic bacteria, Vibrio sp.

A randomized, double-blind, placebo-controlled study of polyunsaturated fatty acids efficacy in adolescents with anorexia nervosa

2020 ◽  
Vol 36 (2) ◽  
pp. 95-106
Author(s):  
Agnieszka M. Piróg-Balcerzak ◽  
Anna K. Bażyńska ◽  
Katarzyna Biernacka ◽  
Joanna Brągoszewska ◽  
Lidia Popek ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Anorexia Nervosa ◽  
Polyunsaturated Fatty Acids ◽  
Small Sample Size ◽  
Small Sample ◽  
Female Adolescent ◽  
Eating Attitude ◽  
Controlled Study ◽  
Omega 3 ◽  
Eat 26

Objective. Omega–3 polyunsaturated fatty acids (PUFAs) were tested in adolescent depression and in several neurodevelopmental disorders with partial success. Anorexia nervosa (AN) is characterised by deficiencies in fatty food intake and frequent comorbidity, including depressive and cognitive symptoms. Thus supplementation with PUFAs may be beneficial in this group of patients. The aim of the study was to assess whether PUFAs as an add-on treatment is associated with better improvement of body mass index (BMI) and psychopathological symptoms than placebo in patients with AN. Method. 61 female adolescent inpatients with AN were randomly allocated to omega–3 PUFAs supplementation or placebo for 10 weeks. Patients also participated in the behavioural programme and eclectic psychotherapy (treatment as usual, TAU). At baseline and follow-up visits, patients’ BMI and psychopathology were assessed with Clinical Global Impression Scale (CGI), Patient Global Impression Scale (PGI), and Eating Attitude Test (EAT-26). Results. After 10 weeks, both groups showed improvement in all parameters. Improvement in CGI scores was observed greater in placebo vs. PUFA-s group (p = 0.015) while other differences were not statistically significant. Omega–3 PUFAs supplementation appears not to be effective as an add-on treatment in inpatient adolescent girls with anorexia nervosa. Conclusions. The results should be analysed with caution due to small sample size and heterogeneity in TAU. As the TAU turned out to be highly effective, additional therapeutic effect of PUFA might not be visible. Nevertheless, that does not explain the tendency for better improvement in the placebo group.

Polyunsaturated fatty acids and phytoestrogens modulate osteoclastogenesis and bone resorption in raw 264.7 macrophages

2013 ◽  
Author(s):  
Natalie Shepherd ◽  
Jager Cassandre De ◽  
Abe Kasonga ◽  
Sumari Marais ◽  
Yuko Tousen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Bone Resorption ◽  
Polyunsaturated Fatty Acids ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages

Metabolic immunomodulation: polyunsaturated fatty acids for the prevention of acute respiratory pathology in children

2017 ◽  
Vol 81 (1) ◽  
pp. 64-73
Author(s):  
L.S. Ovcharenko ◽  
◽  
T.V. Slutskaya ◽  
A.A. Vertegel ◽  
T.G. Andrienko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Respiratory Pathology
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