scholarly journals Rapid detection of abrin in foods with an up-converting phosphor technology-based lateral flow assay

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiao Liu ◽  
Yong Zhao ◽  
Chongyun Sun ◽  
Xiaochen Wang ◽  
Xinrui Wang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals Evaluation of an Immunochromatographic Lateral Flow Assay (OXA-48K-SeT) for Rapid Detection of OXA-48-Like Carbapenemases in Enterobacteriaceae

2015 ◽  
Vol 54 (2) ◽  
pp. 471-473 ◽  
Author(s):  
David W. Wareham ◽  
Rishita Shah ◽  
Jonathan W. Betts ◽  
Lynette M. Phee ◽  
Muhd Haziq F. Abdul Momin
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Solid Medium ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Content Type

We evaluated an immunochromatographic lateral flow assay to detect OXA-48-like carbapenemases (OXA-48K-SeT) inEnterobacteriaceae(n= 82). One hundred percent sensitivity and specificity were observed using bacteria recovered from both solid medium and spiked blood culture bottles, and the results were obtained in <10 min.

Highly sensitive and rapid detection of porcine circovirus 2 by avidin-biotin complex based lateral flow assay coupled to isothermal amplification

2021 ◽  
Author(s):  
Minju Jang ◽  
SeJin Kim ◽  
Junkyu Song ◽  
Sanghyo Kim
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Lateral Flow Assay ◽  
Detection Sensitivity ◽  
Porcine Circovirus ◽  
Highly Sensitive ◽  
Porcine Circovirus 2 ◽  
Avidin Biotin Complex

In this study, a new platform for the detection of porcine circovirus 2 was developed by avidin-biotin complex based lateral flow assay (LAMP-LFA). Improved detection sensitivity was attained by using...

scholarly journals Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis

2001 ◽  
Vol 8 (1) ◽  
pp. 166-169 ◽  
Author(s):  
Henk L. Smits ◽  
C. K. Eapen ◽  
Sheela Sugathan ◽  
Mariamma Kuriakose ◽  
M. Hussein Gasem ◽  
...  
Keyword(s):  
Positive Result ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Test Line ◽  
Lateral Flow ◽  
Immunoglobulin M ◽  
Lateral Flow Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Immunoglobulin ◽  
Human Sera

ABSTRACT An assay device for the rapid detection ofLeptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.

A Time-Resolved-Fluorescence Lateral Flow Assay for Rapid Detection of Cholyglycine Acid for the Diagnosis of Liver Diseases

2021 ◽  
Vol 11 (10) ◽  
pp. 2023-2029
Author(s):  
Wang Zhang ◽  
Yujun Zhou ◽  
Xiuli Xu ◽  
Yaping Tian ◽  
Chunyan Zhang
Keyword(s):  
Rapid Detection ◽  
Liver Diseases ◽  
Point Of Care ◽  
Quantitative Information ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Homogeneous Enzyme Immunoassay

Liver disease is a great danger to human health. The determination of blood level of Cholyglycine acid (CG) is a vital biomarker for the assessment of liver function in clinic, which is contribute to the diagnosis of liver diseases. Thus, establishing accuracy, rapid and convenient method for the detection of glycolic acid is of great significance. In this study, a time-resolved-fluorescence (TRF) lateral flow assay for rapid detection of CG was development. The analytical detection limit (mean of zero-2 SD) was 0.06 μg/mL The method showed good linearity in the range of 0.2–40 g/mL and was not affected by biomolecules with similar structure to CG. The analytical mean recovery of control was between 90–110% and the imprecision of intra- and inter-assay of CVs was less than 10%. No significant matrix effect was observed in saline, serum, plasma or whole blood. A good correlation was found with the homogeneous enzyme immunoassay (HEIA) assay (slopes 1.0463, y-intercepts 0.2721 μg/mL, R = 0.989, n = 50, P < 0.001). The CG TRF analysis could provide reproducible and quantitative information about the state of liver in a few minutes, which is suitable for the detection of liver diseases in point-of-care-testing (POCT) conditions.

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