scholarly journals Characterization of aspartyl aminopeptidase from Toxoplasma gondii

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jun Zheng ◽  
Ziying Cheng ◽  
Honglin Jia ◽  
Yonghui Zheng
Keyword(s):  
Toxoplasma Gondii ◽  
Aspartyl Aminopeptidase

Characterization of the New Fecal Form of Toxoplasma gondii

1970 ◽  
Vol 56 (3) ◽  
pp. 447 ◽  
Author(s):  
J. P. Dubey ◽  
Nancy L. Miller ◽  
J. K. Frenkel
Keyword(s):  
Toxoplasma Gondii

Detection and characterization of DNA polymerase activity in Toxoplasma gondii

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 135-139 ◽  
Author(s):  
A. Makioka ◽  
B. Stavros ◽  
J. T. Ellis ◽  
A. M. Johnson
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Sedimentation Coefficient ◽  
Polymerase Activity ◽  
Magnesium Ions ◽  
Dna Polymerase Β ◽  
Human Dna ◽  
Approximate Molecular Weight

SUMMARYA DNA polymerase activity has been detected and characterized in crude extracts from tachzoites of Toxoplasma gondii. The enzyme has a sedimentation coefficient of 6·4 S, corresponding to an approximate molecular weight of 150000 assuming a globular shape. Like mammalian DNA polymerase α, the DNA polymerase of T. gondii was sensitive to N-ethylmaleimide and inhibited by high ionic strength. However, the enzyme activity was not inhibited by aphidicolin which is an inhibitor of mammalian DNA polymerases α, δ and ε and also cytosine-β-D-arabinofuranoside-5′-triphosphate which is an inhibitor of α polymerase. The activity was inhibited by 2′,3′-dideoxythymidine-5′-triphosphate which is an inhibitor of mammalian DNA polymerase β and γ. Magnesium ions (Mg2+) were absolutely required for activity and its optimal concentration was 6 mM. The optimum potassium (K+) concentration was 50 mM and a higher concentration of K+ markedly inhibited the activity. Activity was optimal at pH 8. Monoclonal antibodies against human DNA polymerase did not bind to DNA polymerase of T. gondii. Thus the T. gondii enzyme differs from the human enzymes and may be a useful target for the design of toxoplasmacidal drugs.

Genetic characterization of Toxoplasma gondii in wildlife from Alabama, USA

2012 ◽  
Vol 112 (3) ◽  
pp. 1333-1336 ◽  
Author(s):  
Li Yu ◽  
Jilong Shen ◽  
Chunlei Su ◽  
Christine A. Sundermann
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization

Prevalence and genetic characterization of Toxoplasma gondii in donkeys in northeastern China

2017 ◽  
Vol 54 ◽  
pp. 455-457 ◽  
Author(s):  
Xiao-Xuan Zhang ◽  
Wei Shi ◽  
Nian-Zhang Zhang ◽  
Kun Shi ◽  
Jian-Ming Li ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Northeastern China

scholarly journals Genetic Characterization of Toxoplasma gondii Isolates and Toxoplasmosis Seroprevalence in Stray Cats of İzmir, Turkey

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104930 ◽  
Author(s):  
Hüseyin Can ◽  
Mert Döşkaya ◽  
Daniel Ajzenberg ◽  
H. Gökhan Özdemir ◽  
Ayşe Caner ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Stray Cats ◽  
Toxoplasma Gondii Isolates

First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina

2014 ◽  
Vol 63 (2) ◽  
pp. 470-472 ◽  
Author(s):  
Lais Pardini ◽  
Liliana A. Carral ◽  
Mariana Bernstein ◽  
María L. Gos ◽  
Patricia Olejnik ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Human Placenta

Genetic Characterization of Toxoplasma gondii DNA Samples Isolated From Humans Living in North America: An Unexpected High Prevalence of Atypical Genotypes

2018 ◽  
Vol 218 (11) ◽  
pp. 1783-1791 ◽  
Author(s):  
Christelle Pomares ◽  
Sébastien Devillard ◽  
Tyson H Holmes ◽  
Tudor Rares Olariu ◽  
Cynthia J Press ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
North America ◽  
Genetic Characterization ◽  
High Prevalence
Sign in / Sign up

Export Citation Format

Share Document