scholarly journals Mesenchymal Stem/Stromal Cells seeded on cartilaginous endplates promote Intervertebral Disc Regeneration through Extracellular Matrix Remodeling

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Catarina Leite Pereira ◽  
Graciosa Q. Teixeira ◽  
Cláudia Ribeiro-Machado ◽  
Joana Caldeira ◽  
Madalena Costa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Stromal Cells ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Disc Regeneration ◽  
Intervertebral Disc Regeneration

scholarly journals Exogenous Delivery of Link N mRNA into Chondrocytes and MSCs—The Potential Role in Increasing Anabolic Response

2019 ◽  
Vol 20 (7) ◽  
pp. 1716 ◽  
Author(s):  
Gauri Tendulkar ◽  
Sabrina Ehnert ◽  
Vrinda Sreekumar ◽  
Tao Chen ◽  
Hans-Peter Kaps ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cell Types ◽  
Bmp Signaling ◽  
Anabolic Response ◽  
Health And Social Care ◽  
Intervertebral Disc Regeneration ◽  
Exogenous Delivery

Musculoskeletal disorders, such as osteoarthritis and intervertebral disc degeneration are causes of morbidity, which concomitantly burdens the health and social care systems worldwide, with massive costs. Link N peptide has recently been described as a novel anabolic stimulator for intervertebral disc repair. In this study, we analyzed the influence on anabolic response, by delivering synthetic Link N encoding mRNA into primary human chondrocytes and mesenchymal stromal cells (SCP1 cells), Furthermore, both cell types were seeded on knitted titanium scaffolds, and the influence of Link N peptide mRNA for possible tissue engineering applications was investigated. Synthetic modified Link N mRNA was efficiently delivered into both cell types and cell transfection resulted in an enhanced expression of aggrecan, Sox 9, and type II collagen with a decreased expression of type X collagen. Interestingly, despite increased expression of BMP2 and BMP7, BMP signaling was repressed and TGFβ signaling was boosted by Link N transfection in mesenchymal stromal cells, suggesting possible regulatory mechanisms. Thus, the exogenous delivery of Link N peptide mRNA into cells augmented an anabolic response and thereby increased extracellular matrix synthesis. Considering these findings, we suppose that the cultivation of cells on knitted titanium scaffolds and the exogenous delivery of Link N peptide mRNA into cells could mechanically support the stability of tissue-engineered constructs and improve the synthesis of extracellular matrix by seeded cells. This method can provide a potent strategy for articular cartilage and intervertebral disc regeneration.

Theranostic radiopharmaceuticals targeting cancer-associated fibroblasts

2020 ◽  
Vol 13 ◽  
Author(s):  
Fedor Zhuravlev
Keyword(s):  
Extracellular Matrix ◽  
Immune Evasion ◽  
Stromal Cells ◽  
Tumor Stroma ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Cancer Associated Fibroblasts ◽  
Malignant Cells ◽  
Anti Cancer ◽  
Tumor Growth And Metastasis

The tumor microenvironment is a dynamic ecosystem where malignant cells interact with the stromal cells sustaining and promoting tumor growth and metastasis. Cancer-associated fibroblasts (CAFs) are the major component of tumor stroma. CAFs control key tumorigenic activities by participating in immune evasion and suppression, extracellular matrix remodeling, neo-angiogenesis, and drug resistance. Therefore, targeting CAFs emerges as an attractive anti-cancer strategy. This review summarized recent advancements in targeting CAFs with diagnostic and therapeutic radiopharmaceuticals using clinically-promising biomarkers. The efforts to improve clinical outcomes via application of new radiotheranostic compounds are discussed in the context of radionuclide, the pharmacophore, and, more generally, in terms of biomarker specificity and expression across different cancers and CAF phenotypes.

BMP 7 enhances the effect of BMSC s on extracellular matrix remodeling in a rabbit model of intervertebral disc degeneration

FEBS Journal ◽  
2016 ◽  
Vol 283 (9) ◽  
pp. 1689-1700 ◽  
Author(s):  
Jun Xu ◽  
Xiao‐Qiang E ◽  
Nan‐Xiang Wang ◽  
Mo‐Nan Wang ◽  
Huan‐Xin Xie ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Rabbit Model ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling

scholarly journals Heparanase isoform expression and extracellular matrix remodeling in intervertebral disc degenerative disease

Clinics ◽  
2011 ◽  
Vol 66 (5) ◽  
pp. 903-909 ◽  
Author(s):  
Luciano Miller Reis Rodrigues ◽  
Thérèse Rachell Theodoro ◽  
Leandro Luongo Matos ◽  
Ana Maria Mader ◽  
Carlo Milani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intervertebral Disc ◽  
Degenerative Disease ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Isoform Expression

scholarly journals Cross-Talk Between Human Tenocytes and Bone Marrow Stromal Cells Potentiates Extracellular Matrix Remodeling In Vitro

2015 ◽  
Vol 117 (3) ◽  
pp. 684-693 ◽  
Author(s):  
Emmanuel C. Ekwueme ◽  
Jay V. Shah ◽  
Mahir Mohiuddin ◽  
Corina A. Ghebes ◽  
João F. Crispim ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Cross Talk ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling
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