scholarly journals Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kengo Kanazaki ◽  
Kohei Sano ◽  
Akira Makino ◽  
Tsutomu Homma ◽  
Masahiro Ono ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Photoacoustic Imaging

scholarly journals Multifunctional Nanobubbles Carrying Indocyanine Green and Paclitaxel for Molecular Imaging and Treatment of Prostate Cancer

2020 ◽  
Author(s):  
Minmin Lan ◽  
Lianhua Zhu ◽  
Yixuan Wang ◽  
Dajia Shen ◽  
Kejing Fang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Indocyanine Green ◽  
Ultrasound Imaging ◽  
Multimodal Imaging ◽  
Photoacoustic Imaging ◽  
Drug Loading ◽  
Tissue Imaging ◽  
Tumor Xenografts

Abstract Background: Combining ultrasound imaging with photoacoustic imaging provides tissue imaging with high contrast and high resolution, thereby enabling rapid and direct measurement and tracking of tumor growth and metastasis. At the same time, ultrasound targeted nanobubble destruction (UTND) provides an effective way to drug accumulation; effectively increasing the content of the drug in the tumor area and reducing potential side effects, thereby contributing effectively to the treatment of the tumors.Result: In this study, we prepared multifunctional nanobubbles (NBs) carrying indocyanine green (ICG) and paclitaxel (PTX) (ICG-PTX NBs), and study their application in ultrasound imaging of prostate cancer as well as their therapeutic effect on prostate cancer when combined with ultrasound targeted nanobubble destruction (UTND). ICG-PTX NBs were prepared by mechanical oscillation method. Their particle size and Zeta potential of ICG-PTX NBs were 469.5±32.87 nm and -21.70±1.222 mV, respectively. The encapsulation efficiency and drug loading efficiency of ICG were 68% and 6.2%, respectively. In vitro imaging experiments showed that ICG-PTX NBs were highly amenable to multimodal imaging including ultrasound, photoacoustic and fluorescence imaging, and the imaging effect is positively correlated with their concentration. The imaging effect of tumor xenografts also indicated that ICG-PTX NBs were of good use for multimodal imaging. In experiments testing the growth of PC-3 cells in vitro and tumor xenografts in vivo, the ICG-PTX NBs+US group showed more significant inhibition of cell proliferation and promotion of cell apoptosis compared to the other groups (P < 0.05). Blood biochemical analysis of the six groups showed that the levels of aspartate aminotransferase (AST), phenylalanine aminotransferase (ALT), serum creatinine (CRE) and blood urea nitrogen (BUN) in the ICG-PTX NBs group and the ICG-PTX NBs+US group were significantly lower than those in the PTX group (P < 0.05). Moreover, H&E staining of tissue sections from vital organ showed no obvious abnormalities in the ICG-PTX NBs group and the ICG-PTX NBs+US group.Conclusions: ICG-PTX NBs can be used as a non-invasive, pro-apoptotic contrast agent for both diagnosis and treatment that can achieve enhanced imaging, effective inhibition and killing of prostate cancer under the guidance of multimodal imaging including ultrasound, fluorescence and photoacoustic imaging.

scholarly journals Indocyanine green labeling for optical and photoacoustic imaging of mesenchymal stem cells after in vivo transplantation

2019 ◽  
Vol 12 (5) ◽  
Author(s):  
Miriam Filippi ◽  
Francesca Garello ◽  
Chiara Pasquino ◽  
Francesca Arena ◽  
Pierangela Giustetto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Indocyanine Green ◽  
Photoacoustic Imaging

scholarly journals A human photoacoustic imaging reporter gene using the clinical dye indocyanine green

2019 ◽  
Author(s):  
Nivin N. Nyström ◽  
Lawrence C.M. Yip ◽  
Jeffrey J.L. Carson ◽  
Timothy J. Scholl ◽  
John A. Ronald
Keyword(s):  
Indocyanine Green ◽  
Cell Tracking ◽  
Photoacoustic Imaging ◽  
Organic Anion ◽  
Reporter System ◽  
Optical Contrast ◽  
Organic Anion Transporting Polypeptide ◽  
Gene Therapies

ABSTRACTPhotoacoustic imaging (PAI) combines optical contrast with the resolution and depth-detection of ultrasound and is increasingly being utilized for medical imaging in patients. PAI reporter genes would allow for monitoring of cell and gene therapies, but current reporters have immunogenicity and/or toxicity concerns that may limit clinical translation. Here we report a PAI reporter system employing the ability of human organic anion transporting polypeptide 1b3 (Oatp1b3) to take up the clinical dye indocyanine green (ICG) into cells. Following ICG administration, cells synthetically expressing Oatp1b3 exhibited significantly increased PAI signals compared to control cells both in vitro and in mice. Several benefits of this technology are the human derivation of Oatp1b3, and the high extinction coefficient, low quantum yield and pre-existing clinical approval of ICG. We posit that the Oatp1b3-ICG reporter system could be useful for in vivo gene and cell tracking in preclinical and clinical applications.

Activable Nanoparticle for Tumor Aggressiveness and Drug Resistance Prediction by Glutathione Heterogeneous Imaging

2021 ◽  
Vol 17 (7) ◽  
pp. 1339-1348
Author(s):  
Yaqin Wang ◽  
Wenting Shang ◽  
Jianping Xiong ◽  
Yu Liu ◽  
Ting Luo ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Indocyanine Green ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Drug Resistant ◽  
Tumor Aggressiveness ◽  
Tumor Invasiveness ◽  
Drug Resistance Prediction ◽  
Resistance Prediction

Studies have shown that a higher GSH level is related to more drug-resistant and invasiveness of a tumor. However, it is a great challenge to accurately imaging the GSH level in vivo, for its imaging intensity will interfered by different accumulation of probes in the tumor. Thus, we hypothesized ratiometric photoacoustic imaging that can be used to predict the drug-resistant and invasiveness of tumors by accurate GSH level imaging. In this study, we synthesized MnO2/Indocyanine Green (MnO2/ICG). It can be used as ratiometric photoacoustic (PA) imaging probe, for its absorption at 780 nm (Ab780) can be decreased and 680 nm (Ab680) remain unchanged upon GSH reduction. And our results confirmed that a lower PA absorption ratio (Ab780/Ab680) corresponded to a higher GSH level of the tumor. Besides, the near-infrared fluorescence (FI) imaging was used as an assistant, for it can be quenched upon GSH. Therefore, MnO2/ICG can predict the tumor invasiveness and drug resistance by imaging the GSH level. This study provides reference to predict the prognosis of tumors by imaging the metabolic biomarker of tumors.

Development of mesoporous silica-based nanoprobes for optical bioimaging applications

2021 ◽  
Author(s):  
Bo Sun ◽  
Xu Zhen ◽  
Xiqun Jiang
Keyword(s):  
Mesoporous Silica ◽  
Fluorescence Imaging ◽  
Photoacoustic Imaging

This review mainly introduced the MSNs-based nanoprobes for in vivo bioimaging applications including fluorescence imaging and photoacoustic imaging.

scholarly journals In Vitro and In Vivo Multispectral Photoacoustic Imaging for the Evaluation of Chromophore Concentration

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3366
Author(s):  
Aneline Dolet ◽  
Rita Ammanouil ◽  
Virginie Petrilli ◽  
Cédric Richard ◽  
Piero Tortoli ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Photoacoustic Imaging ◽  
Hyperspectral Remote Sensing ◽  
Reference Spectrum ◽  
In Vivo Experiments ◽  
Mouse Tumors ◽  
Chromophore Concentration ◽  
Saturation Rate

Multispectral photoacoustic imaging is a powerful noninvasive medical imaging technique that provides access to functional information. In this study, a set of methods is proposed and validated, with experimental multispectral photoacoustic images used to estimate the concentration of chromophores. The unmixing techniques used in this paper consist of two steps: (1) automatic extraction of the reference spectrum of each pure chromophore; and (2) abundance calculation of each pure chromophore from the estimated reference spectra. The compared strategies bring positivity and sum-to-one constraints, from the hyperspectral remote sensing field to multispectral photoacoustic, to evaluate chromophore concentration. Particularly, the study extracts the endmembers and compares the algorithms from the hyperspectral remote sensing domain and a dedicated algorithm for segmentation of multispectral photoacoustic data to this end. First, these strategies are tested with dilution and mixing of chromophores on colored 4% agar phantom data. Then, some preliminary in vivo experiments are performed. These consist of estimations of the oxygen saturation rate (sO2) in mouse tumors. This article proposes then a proof-of-concept of the interest to bring hyperspectral remote sensing algorithms to multispectral photoacoustic imaging for the estimation of chromophore concentration.

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