scholarly journals Generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Honghui Li ◽  
Gui Wang ◽  
Zhiqiang Hao ◽  
Guozhong Zhang ◽  
Yubo Qing ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Gene Editing ◽  
Knock Out

scholarly journals Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Yajun Yin ◽  
Haiyang Hao ◽  
Xingbin Xu ◽  
Liangcai Shen ◽  
Wenjing Wu ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Knock Out

scholarly journals Cloning of Homozygous a1,3-Galactosyltransferase Gene Knock-Out Pigs by Somatic Cell Nuclear Transfer

10.5772/29267 ◽  
2012 ◽  
Author(s):  
Hitomi Matsunari ◽  
Masahito Watanabe ◽  
Kazuhiro Umeyama ◽  
Kazuaki Nakano ◽  
Yuka Ikezawa ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Knock Out

110 Development competence of β-lactoglobulin gene editing bovine embryos producing by CRISPR/Cas9 and somatic cell nuclear transfer

2022 ◽  
Vol 34 (2) ◽  
pp. 292
Author(s):  
E. N. Shedova ◽  
G. N. Singina ◽  
V. P. Sergiev ◽  
M. P. Rubtsova ◽  
N. V. Ravin ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Gene Editing ◽  
Bovine Embryos ◽  
Β Lactoglobulin

scholarly journals Production of a Cloned Offspring and CRISPR/Cas9 Genome Editing of Embryonic Fibroblasts in Cattle

2021 ◽  
Vol 496 (1) ◽  
pp. 48-51
Author(s):  
G. N. Singina ◽  
P. V. Sergiev ◽  
A. V. Lopukhov ◽  
M. P. Rubtsova ◽  
N. P. Taradajnic ◽  
...  
Keyword(s):  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Gene Editing ◽  
Protein Gene ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
Fibroblast Cell Line ◽  
Embryonic Fibroblasts ◽  
Knock Out ◽  
Beta Lactoglobulin

Abstract Somatic Cell Nuclear Transfer (SCNT) technique was used to produce the first viable cloned cattle offspring in Russia. Whole-genome SNP genotyping confirmed that the cloned calf was identical to the fibroblast cell line that was used for SCNT. CRISPR/Cas9 approach was subsequently used to knock out genes for beta-lactoglobulin gene (PAEP) and the beta-lactoglobulin-like protein gene (LOC100848610) in the fibroblast cells. Gene editing (GE) efficiency was 4.4% for each of these genes. We successfully obtained single-cell-derived fibroblast colonies containing PAEP and LOC100848610 knockouts, which will be used to produce beta-lactoglobulin-deficient cattle.

scholarly journals Author Correction: Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Effrosyni Fatira ◽  
Miloš Havelka ◽  
Catherine Labbé ◽  
Alexandra Depincé ◽  
Viktoriia Iegorova ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer
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