scholarly journals Adenovirus-mediated expression of orphan nuclear receptor NR4A2 targeting hepatic stellate cell attenuates liver fibrosis in rats

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pengguo Chen ◽  
Jie Li ◽  
Yan Huo ◽  
Jin Lu ◽  
Lili Wan ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Nuclear Receptor ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Orphan Nuclear Receptor

scholarly journals Orphan nuclear receptor NR4A2 inhibits hepatic stellate cell proliferation through MAPK pathway in liver fibrosis

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1518 ◽  
Author(s):  
Pengguo Chen ◽  
Jie Li ◽  
Yan Huo ◽  
Jin Lu ◽  
Lili Wan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Proliferation ◽  
Liver Fibrosis ◽  
Nuclear Receptor ◽  
Mapk Pathway ◽  
Stellate Cell ◽  
Smooth Muscle Actin ◽  
Control Group ◽  
Orphan Nuclear Receptor ◽  
Fibrotic Liver

Hepatic stellate cells (HSCs) play a crucial role in liver fibrosis, which is a pathological process characterized by extracellular matrix accumulation. NR4A2 is a nuclear receptor belonging to the NR4A subfamily and vital in regulating cell growth, metabolism, inflammation and other biological functions. However, its role in HSCs is unclear. We analyzed NR4A2 expression in fibrotic liver and stimulated HSCs compared with control group and studied the influence on cell proliferation, cell cycle, cell apoptosis and MAPK pathway after NR4A2 knockdown. NR4A2 expression was examined by real-time polymerase chain reaction, Western blotting, immunohistochemistry and immunofluorescence analyses. NR4A2 expression was significantly lower in fibrotic liver tissues and PDGF BB or TGF-βstimulated HSCs compared with control group. After NR4A2 knockdownα-smooth muscle actin and Col1 expression increased. In addition, NR4A2 silencing led to the promotion of cell proliferation, increase of cell percentage in S phase and reduced phosphorylation of ERK1/2, P38 and JNK in HSCs. These results indicate that NR4A2 can inhibit HSC proliferation through MAPK pathway and decrease extracellular matrix in liver fibrogenesis. NR4A2 may be a promising therapeutic target for liver fibrosis.

scholarly journals Induction of tropomyosin during hepatic stellate cell activation and the progression of liver fibrosis

2008 ◽  
Vol 3 (2) ◽  
pp. 378-383 ◽  
Author(s):  
Kohji Otogawa ◽  
Tomohiro Ogawa ◽  
Ryoko Shiga ◽  
Kazuo Ikeda ◽  
Norifumi Kawada
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Stellate Cell ◽  
Cell Activation ◽  
Stellate Cell ◽  
Hepatic Stellate Cell Activation

Hepatic Stellate Cell as a Mac‐2‐binding Protein‐producing Cell in Patients with Liver Fibrosis

2021 ◽  
Author(s):  
Dolgormaa Gantumur ◽  
Norifumi Harimoto ◽  
Ryo Muranushi ◽  
Kouki Hoshino ◽  
Chingun Batbayar ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Binding Protein
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