scholarly journals Ablation of Y1 receptor impairs osteoclast bone-resorbing activity

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Daniela M. Sousa ◽  
Francisco Conceição ◽  
Diana I. Silva ◽  
Luís Leitão ◽  
Estrela Neto ◽  
...  
Keyword(s):  
Y1 Receptor ◽  
Bone Resorbing Activity

Co-purification of bone resorbing activity and adenylate cyclase stimulating activity from human tumours associated with the humoral hypercalcaemia of malignancy

1987 ◽  
Vol 114 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Chohei Shigeno ◽  
Itsuo Yamamoto ◽  
Shegiharu Dokoh ◽  
Megumu Hino ◽  
Jun Aoki ◽  
...  
Keyword(s):  
Bone Resorption ◽  
High Performance ◽  
Basic Protein ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Protein Factor ◽  
Human Tumours ◽  
Acid Stable ◽  
Bone Resorbing Activity

Abstract. We have partially purified a tumour factor capable of stimulating both bone resorption in vitro and cAMP accumulation in osteoblastic ROS 17/2 cells from three human tumours associated with humoral hypercalcaemia of malignancy. Purification of tumour factor by sequential acid urea extraction, gel filtration and cation-exchange chromatography, reverse-phase high performance liquid chromatography followed by analytical isoelectric focussing provided a basic protein (pI > 9.3) with a molecular weight of approximately 13 000 as a major component of the final preparation which retained both the two bioactivities. Bone resorbing activity and cAMP-increasing activity in purified factor correlated with each other. cAMP-increasing activity of the factor was heat- and acid-stable, but sensitive to alkaline ambient pH. Treatment with trypsin destroyed cAMP-increasing activity of the factor. Synthetic parathyroid hormone (PTH) antagonist, human PTH-(3– 34) completely inhibited the cAMP-increasing activity of the factor. The results suggest that this protein factor, having its effects on both osteoclastic and osteoblastic functions, may be involved in development of enhanced bone resorption in some patients with humoral hypercalcaemia of malignancy.

Neuropetide Y Y1 receptor deletion impairs matrix demineralization and resorption

2016 ◽  
Author(s):  
Daniela M Sousa ◽  
Francisco Conceicao ◽  
Luis Leitao ◽  
Estrela Neto ◽  
Cecilia J Alves ◽  
...  
Keyword(s):  
Y1 Receptor

Mechanical Strain Effect on Bone-Resorbing Activity and Messenger RNA Expressions of Marker Enzymes in Isolated Osteoclast Culture

2001 ◽  
Vol 16 (4) ◽  
pp. 722-730 ◽  
Author(s):  
Kosaku Kurata ◽  
Toshimasa Uemura ◽  
Atsuko Nemoto ◽  
Tetsuya Tateishi ◽  
Teruo Murakami ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Mechanical Strain ◽  
Strain Effect ◽  
Marker Enzymes ◽  
Bone Resorbing Activity

Regulation of food intake by neuropeptide Y in goldfish

2000 ◽  
Vol 279 (3) ◽  
pp. R1025-R1034 ◽  
Author(s):  
Yuwaraj K. Narnaware ◽  
Pierre P. Peyon ◽  
Xinwei Lin ◽  
Richard E. Peter
Keyword(s):  
Food Intake ◽  
Neuropeptide Y ◽  
Mrna Levels ◽  
Food Ration ◽  
Y1 Receptor ◽  
Daily Food ◽  
Daily Food Ration ◽  
Brain Ventricle ◽  
Dose Dependent Increase ◽  
Dose Dependent

In mammals, neuropeptide Y (NPY) is a potent orexigenic factor. In the present study, third brain ventricle (intracerebroventricular) injection of goldfish NPY (gNPY) caused a dose-dependent increase in food intake in goldfish, and intracerebroventricular administration of NPY Y1-receptor antagonist BIBP-3226 decreased food intake; the actions of gNPY were blocked by simultaneous injection of BIBP-3226. Goldfish maintained on a daily scheduled feeding regimen display an increase in NPY mRNA levels in the telencephalon-preoptic area and hypothalamus shortly before feeding; however, a decrease occured in optic tectum-thalamus. In both fed and unfed fish, brain NPY mRNA levels decreased after scheduled feeding. Restriction in daily food ration intake for 1 wk or food deprivation for 72 h resulted in increased brain NPY mRNA levels. Results from these studies demonstrate that NPY is a physiological brain signal involved in feeding behavior in goldfish, mediating its effects, at least in part, through Y1-like receptors in the brain.

Concanavalin A Directly Stimulates Bone-Resorbing Activity of Osteoclasts and Their Gene Expression of Cathepsin K/OC-2

1997 ◽  
Vol 234 (3) ◽  
pp. 600-604 ◽  
Author(s):  
Shinji Kakudo ◽  
Hiroshi Mano ◽  
Miho Shiokawa ◽  
Yoshihisa Mori ◽  
Masayoshi Kumegawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Concanavalin A ◽  
Cathepsin K ◽  
Bone Resorbing Activity
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