scholarly journals Biochemical and structural characterization of the interface mediating interaction between the influenza A virus non-structural protein-1 and a monoclonal antibody

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jianping Wu ◽  
Chee-Keng Mok ◽  
Vincent Tak Kwong Chow ◽  
Y. Adam Yuan ◽  
Yee-Joo Tan
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Structural Characterization ◽  
Influenza A ◽  
Structural Protein

scholarly journals A NS1-binding monoclonal antibody interacts with two residues that are highly conserved in seasonal as well as newly emerged influenza A virus

2019 ◽  
Vol 77 (1) ◽  
Author(s):  
Su Hui Catherine Teo ◽  
Jian-Ping Wu ◽  
Chee-Keng Mok ◽  
Yee-Joo Tan
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Influenza A ◽  
Rna Binding ◽  
Intracellular Localization ◽  
Cross Reactivity ◽  
Structural Protein ◽  
Multifunctional Protein ◽  
Good Target ◽  
Conformational Plasticity

Abstract The non-structural protein 1 (NS1) of influenza A virus (IAV) is a multifunctional protein that antagonizes host antiviral responses, modulating virus pathogenesis. As such, it serves as a good target for research and diagnostic assay development. In this study, we have generated a novel monoclonal antibody (mAb) 19H9 and epitope mapping revealed that two residues, P85 and Y89, of NS1 are essential for interacting with this mAb. Furthermore, residues P85 and Y89 are found to be highly conserved across different IAV subtypes, namely seasonal H1N1 and H3N2, as well as the highly pathogenic H5N1 and H5N6 avian strains. Indeed, mAb 19H9 exhibits broad cross-reactivity with IAV strains of different subtypes. The binding of mAb 19H9 to residue Y89 was further confirmed by the abrogation of interaction between NS1 and p85β. Additionally, mAb 19H9 also detected NS1 proteins expressed in IAV-infected cells, showing NS1 intracellular localization in the cytoplasm and nucleolus. To our knowledge, mAb 19H9 is the first murine mAb to bind at the juxtaposition between the N-terminal RNA-binding domain and C-terminal effector domain of NS1. It could serve as a useful research tool for studying the conformational plasticity and dynamic changes in NS1.

scholarly journals Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets the Fusion Domain of Group 2 Influenza A Virus Hemagglutinin

2014 ◽  
Vol 88 (23) ◽  
pp. 13580-13592 ◽  
Author(s):  
G. S. Tan ◽  
P. S. Lee ◽  
R. M. B. Hoffman ◽  
B. Mazel-Sanchez ◽  
F. Krammer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Influenza A ◽  
Neutralizing Monoclonal Antibody ◽  
Fusion Domain ◽  
Group 2

Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus

2011 ◽  
Vol 91 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Tatsuhiko Ozawa ◽  
Aishun Jin ◽  
Kazuto Tajiri ◽  
Masaya Takemoto ◽  
Tomoko Okuda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Influenza A ◽  
Matrix Protein ◽  
Human Monoclonal Antibody ◽  
Extracellular Domain

The In Situ Structural Characterization of the Influenza A Virus Matrix M1 Protein within a Virion

2009 ◽  
Vol 16 (11) ◽  
pp. 1407-1413 ◽  
Author(s):  
Alexander Shishkov ◽  
Elena Bogacheva ◽  
Alexey Dolgov ◽  
Alexey Chulichkov ◽  
Denis Knyazev ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Structural Characterization ◽  
Influenza A ◽  
M1 Protein

scholarly journals Generation and Characterization of the Monoclonal Antibody Panel Specific to the NS1 Protein of the Influenza A Virus

2018 ◽  
Vol 54 (7) ◽  
pp. 756-765
Author(s):  
V. Z. Krivitskaya ◽  
E. V. Sorokin ◽  
T. R. Tsareva ◽  
M. V. Sergeeva ◽  
R. A. Kadyrova ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Influenza A Virus ◽  
Influenza A ◽  
Ns1 Protein ◽  
Antibody Panel

scholarly journals Generation and characterization of a new panel of broadly reactive anti-NS1 mAbs for detection of influenza A virus

2013 ◽  
Vol 94 (3) ◽  
pp. 593-605 ◽  
Author(s):  
Md Niaz Rahim ◽  
Mohammed Selman ◽  
Patricia J. Sauder ◽  
Nicole E. Forbes ◽  
William Stecho ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
A549 Cells ◽  
Structural Protein ◽  
Good Target ◽  
Ns3 Protein ◽  
And Function ◽  
Selective Reactivity ◽  
Darby Canine Kidney

Influenza A virus (IAV) non-structural protein 1 (NS1) has multiple functions, is essential for virus replication and may be a good target for IAV diagnosis. To generate broadly cross-reactive NS1-specific mAbs, mice were immunized with A/Hong Kong/1/1968 (H3N2) 6×His-tagged NS1 and hybridomas were screened with glutathione S-transferase-conjugated NS1 of A/Puerto Rico/8/1934 (H1N1). mAbs were isotyped and numerous IgG-type clones were characterized further. Most clones specifically recognized NS1 from various H1N1 and H3N2 IAV types by both immunoblot and immunofluorescence microscopy in mouse M1, canine Madin–Darby canine kidney and human A549 cells. mAb epitopes were mapped by overlapping peptides and selective reactivity to the newly described viral NS3 protein. These mAbs detected NS1 in both the cytoplasm and nucleus by immunostaining, and some detected NS1 as early as 5 h post-infection, suggesting their potential diagnostic use for tracking productive IAV replication and characterizing NS1 structure and function. It was also demonstrated that the newly identified NS3 protein is localized in the cytoplasm to high levels.

scholarly journals Domain-Domain Interactions in the Self-Assembly of Non-Structural Protein 1 from Influenza a Virus

2021 ◽  
Vol 120 (3) ◽  
pp. 23a
Author(s):  
James E. Gonzales ◽  
Jie Shi ◽  
Jae-Hyun Cho ◽  
Wonmuk Hwang
Keyword(s):  
Influenza A Virus ◽  
Self Assembly ◽  
Influenza A ◽  
The Self ◽  
Structural Protein ◽  
Domain Interactions
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