scholarly journals Erratum: Global transcriptomic responses of Escherichia coli K-12 to volatile organic compounds

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Pui Yi Yung ◽  
Letizia Lo Grasso ◽  
Abeed Fatima Mohidin ◽  
Enzo Acerbi ◽  
Jamie Hinks ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic ◽  
Transcriptomic Responses ◽  
K 12

scholarly journals Global transcriptomic responses of Escherichia coli K-12 to volatile organic compounds

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pui Yi Yung ◽  
Letizia Lo Grasso ◽  
Abeed Fatima Mohidin ◽  
Enzo Acerbi ◽  
Jamie Hinks ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Stress Responses ◽  
Regulatory Elements ◽  
Promoter Fusion ◽  
Volatile Organic ◽  
Transcriptomic Responses ◽  
K 12 ◽  
Genetic Responses

Abstract Volatile organic compounds (VOCs) are commonly used as solvents in various industrial settings. Many of them present a challenge to receiving environments, due to their toxicity and low bioavailability for degradation. Microorganisms are capable of sensing and responding to their surroundings and this makes them ideal detectors for toxic compounds. This study investigates the global transcriptomic responses of Escherichia coli K-12 to selected VOCs at sub-toxic levels. Cells grown in the presence of VOCs were harvested during exponential growth, followed by whole transcriptome shotgun sequencing (RNAseq). The analysis of the data revealed both shared and unique genetic responses compared to cells without exposure to VOCs. Results suggest that various functional gene categories, for example, those relating to Fe/S cluster biogenesis, oxidative stress responses and transport proteins, are responsive to selected VOCs in E. coli. The differential expression (DE) of genes was validated using GFP-promoter fusion assays. A variety of genes were differentially expressed even at non-inhibitory concentrations and when the cells are at their balanced-growth. Some of these genes belong to generic stress response and others could be specific to VOCs. Such candidate genes and their regulatory elements could be used as the basis for designing biosensors for selected VOCs.

Effect of volatile organic compounds from Pinus sylvestris and Picea abies on Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae and Salmonella enterica serovar Typhimurium

Holzforschung ◽  
2017 ◽  
Vol 71 (11) ◽  
pp. 905-912 ◽  
Author(s):  
Tiina Vainio-Kaila ◽  
Tuomas Hänninen ◽  
Aino Kyyhkynen ◽  
Martin Ohlmeyer ◽  
Anja Siitonen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Antibacterial Effect ◽  
Bacterial Strains ◽  
Volatile Organic ◽  
Wood Particles ◽  
Serovar Typhimurium

AbstractPine and spruce heartwood and sapwood were milled to wood particles and the volatile organic compounds (VOCs) of the wood particles were tested against four bacterial strains. To study the influence of relative humidity on the antibacterial effect, both dry and wet wood particles were tested. Twenty microliters of the bacterial dilution with a concentration of 1.5×107CFU ml−1was cultured on glass surfaces in the presence of VOCs and the amount of viable bacteria was studied after 2, 4, and 24 h. The volatile emissions were evaluated by GC/MS and the results were compared with the results from the bacterial trial. VOCs had an antibacterial effect onEscherichia coli, Streptococcus pneumoniaeand slightly onSalmonella entericaserovar Typhimurium. But the effect onStaphylococcus aureuswas minute even after 3 days’ incubation. The dry wood particles generally had a stronger antibacterial effect, though the amount of VOCs from the wet wood was higher. Pine heartwood had the strongest antibacterial effect and also the highest emissions of VOCs. However, the interaction between different bacterial strains and wood species shows some variations.

scholarly journals Application of a Micro-Machined Electronic Nose to Detect Escherichia Coli in Human Urine Samples

Proceedings ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 3
Author(s):  
Soprani ◽  
Zambotti ◽  
Gobbi ◽  
Ponzoni
Keyword(s):  
Escherichia Coli ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Electronic Nose ◽  
Human Urine ◽  
Urine Samples ◽  
Disease Biomarkers ◽  
Human Urine Samples ◽  
Volatile Organic

The analysis of volatile organic compounds (VOCs) as disease biomarkers released by the urine [...]

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