scholarly journals Pre-B-cell colony-enhancing factor protects against apoptotic neuronal death and mitochondrial damage in ischemia

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xiaowan Wang ◽  
Hailong Li ◽  
Shinghua Ding
Keyword(s):  
B Cell ◽  
Neuronal Death ◽  
Mitochondrial Damage ◽  
Enhancing Factor ◽  
Apoptotic Neuronal Death ◽  
Cell Colony

The role of Pre-B-cell colony-enhancing factor in neuronal death after ischemia and motor dysfunction

2016 ◽  
Author(s):  
◽  
Xiaowan Wang
Keyword(s):  
Ischemic Stroke ◽  
Cerebral Ischemia ◽  
Motor Neuron ◽  
B Cell ◽  
Neuronal Death ◽  
Molecular Mechanisms ◽  
Projection Neuron ◽  
Nad Biosynthesis ◽  
Enhancing Factor ◽  
Cell Colony

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Pre-B-cell colony-enhancing factor (PBEF) is the rate-limiting enzyme in the salvage pathway of mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis via converting nicotinamide (NAM) to nicotinamide mononucleotide (NMN). NAD+ is a molecule playing a pivotal role in the cellular metabolisms and energy production. Moreover, increasingly important roles of PBEF-NAD+ cascade have been reported in diverse neurodegenerative diseases including ischemic stroke, axon degeneration and motor neuron disorders. However, currently, the mechanisms downstream of PBEF are not completely understood. In Chapter one, I reviewed the biological functions of PBEF, the pathology of cerebral ischemia, the potential molecular mechanisms of PBEF mediated neuronal protection during ischemic stroke and motor dysfunctions. Then, in Chapter two and Chapter three, I investigated the role and molecular mechanisms by which PBEF-NAD+ cascade exerts brain protection following ischemia in primary cortical neuronal cultures. Using different approaches including confocal imaging, immunocytochemistry, molecular biology and Western blotting, I found that enhancing PBEF mediated NAD+ biosynthesis effectively reduced glutamate excitotoxicity and oxygen glucose deprivation (OGD) induced neuronal death, AIF translation, caspase-3 activation, mitochondrial fragmentation and impairments of mitochondrial function and biogenesis. Finally, in Chapter four, I investigated the effect of neuronal PBEF on the regulation of the motor system using a novel projection neuron-specific and inducible conditional PBEF knockout mice, i.e., Thy1-YFPCreERT2: Pbeff/f mice, in which deletion of PBEF can be achieved by tamoxifen administration. I observed that ablation of PBEF in the projection neuron resulted in muscle atrophy, progressive motor dysfunctions, impairments of synaptic connection at neuromuscular junctions, motor neuron loss and widespread astrogliosis and microgliosis. These results provide novel insights into the development of potential therapeutic strategies for cerebral ischemia and motor disorders based on PBEF-NAD+ cascade.

scholarly journals Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

1994 ◽  
Vol 14 (2) ◽  
pp. 1431-1437 ◽  
Author(s):  
B Samal ◽  
Y Sun ◽  
G Stearns ◽  
C Xie ◽  
S Suggs ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Stem Cell Factor ◽  
Human Peripheral Blood ◽  
Biological Activities ◽  
In Vitro Assays ◽  
Pokeweed Mitogen ◽  
Interleukin 7 ◽  
Enhancing Factor ◽  
Cell Colony

A novel gene coding for the pre-B-cell colony-enhancing factor (PBEF) has been isolated from a human peripheral blood lymphocyte cDNA library. The expression of this gene is induced by pokeweed mitogen and superinduced by cycloheximide. It is also induced in the T-lymphoblastoid cell line HUT 78 after phorbol ester (phorbol myristate acetate) treatment. The predominant mRNA for PBEF is approximately 2.4 kb long and codes for a 52-kDa secreted protein. The 3' untranslated region of the mRNA has multiple TATT motifs, usually found in cytokine and oncogene messages. The PBEF gene is mainly transcribed in human bone marrow, liver tissue, and muscle. We have expressed PBEF in COS 7 and PA317 cells and have tested the biological activities of the conditioned medium as well as the antibody-purified protein in different in vitro assays. PBEF itself had no activity but synergized the pre-B-cell colony formation activity of stem cell factor and interleukin 7. In the presence of PBEF, the number of pre-B-cell colonies was increased by at least 70% above the amount stimulated by stem cell factor plus interleukin 7. No effect of PBEF was found with cells of myeloid or erythroid lineages. These data define PBEF as a novel cytokine which acts on early B-lineage precursor cells.

scholarly journals Elevated Plasma Level of Visfatin/Pre-B Cell Colony-Enhancing Factor in Patients with Type 2 Diabetes Mellitus

2006 ◽  
Vol 91 (1) ◽  
pp. 295-299 ◽  
Author(s):  
Miao-Pei Chen ◽  
Fu-Mei Chung ◽  
Dao-Ming Chang ◽  
Jack C.-R. Tsai ◽  
Han-Fen Huang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Plasma Level ◽  
B Cell ◽  
Elevated Plasma ◽  
Enhancing Factor ◽  
Cell Colony

Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine

2008 ◽  
Vol 115 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Grit Sommer ◽  
Antje Garten ◽  
Stefanie Petzold ◽  
Annette G. Beck-Sickinger ◽  
Matthias Blüher ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
B Cell ◽  
Hormonal Regulation ◽  
Physiological Role ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Insulin Mimetic ◽  
Enzymatic Function ◽  
Enhancing Factor ◽  
Structure Regulation ◽  
Cell Colony

Over the last few years, it has become obvious that obesity and insulin resistance are linked by a variety of proteins secreted by adipocytes. Visfatin/PBEF (pre-B-cell colony-enhancing factor) has recently been identified as a novel adipokine with insulin-mimetic effects. Furthermore, an enzymatic function has been reported that reveals visfatin/PBEF as Nampt (nicotinamide phosphoribosyltransferase; EC 2.4.2.12.). Moreover, reports on the structure and hormonal regulation of visfatin/PBEF/Nampt have given further insights into its potential physiological role. The present review summarizes studies on visfatin/PBEF/Nampt as a novel adipokine.

Pre-B-cell-colony-enhancing factor is critically involved in thrombin-induced lung endothelial cell barrier dysregulation

2005 ◽  
Vol 70 (3) ◽  
pp. 142-151 ◽  
Author(s):  
Shui Q. Ye ◽  
Li Q. Zhang ◽  
Djanybek Adyshev ◽  
Peter V. Usatyuk ◽  
Alexander N. Garcia ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
B Cell ◽  
Enhancing Factor ◽  
Cell Colony

Pre-B-cell colony-enhancing factor, a novel cytokine of human fetal membranes

2002 ◽  
Vol 187 (4) ◽  
pp. 1051-1058 ◽  
Author(s):  
Simona Ognjanovic ◽  
Gillian D. Bryant-Greenwood
Keyword(s):  
B Cell ◽  
Fetal Membranes ◽  
Enhancing Factor ◽  
Cell Colony

Pre-B cell colony enhancing factor/NAMPT/visfatin and its role in inflammation-related bone disease

2010 ◽  
Vol 690 (1-2) ◽  
pp. 95-101 ◽  
Author(s):  
Alexander R. Moschen ◽  
Sabine Geiger ◽  
Romana Gerner ◽  
Herbert Tilg
Keyword(s):  
B Cell ◽  
Bone Disease ◽  
Enhancing Factor ◽  
Cell Colony
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