scholarly journals High throughput cytotoxicity screening of anti-HER2 immunotoxins conjugated with antibody fragments from phage-displayed synthetic antibody libraries

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shin-Chen Hou ◽  
Hong-Sen Chen ◽  
Hung-Wei Lin ◽  
Wei-Ting Chao ◽  
Yao-Sheng Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Antibody Fragments ◽  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals High throughput discovery of influenza virus neutralizing antibodies from phage-displayed synthetic antibody libraries

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ing-Chien Chen ◽  
Yi-Kai Chiu ◽  
Chung-Ming Yu ◽  
Cheng-Chung Lee ◽  
Chao-Ping Tung ◽  
...  
Keyword(s):  
Influenza Virus ◽  
High Throughput ◽  
Neutralizing Antibodies ◽  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly

2020 ◽  
Author(s):  
Christian Hentrich ◽  
Sarah-Jane Kellmann ◽  
Mateusz Putyrski ◽  
Manuel Cavada ◽  
Hanh Hanuschka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Prototyping ◽  
High Throughput ◽  
High Throughput Screening ◽  
Antibody Fragments ◽  
Surface Immobilization ◽  
E Coli ◽  
Protein Ligation ◽  
Periplasmic Expression ◽  
Antibody Libraries

AbstractAntibodies are essential tools in research and diagnostics. While antibody fragments can be rapidly produced in Escherichia coli, full-length antibodies with an Fc region or antibodies modified with probes are time and labor intensive in production.SpyTag/SpyCatcher protein ligation technology could covalently attach such functionalities to antibody fragments equipped with a SpyTag. However, we found that the necessarily periplasmic expression of such antibody fragments in E. coli led to rapid cleavage of the SpyTag by proteases.Here we show how this cleavage can be prevented, making the SpyTag technology accessible for E. coli produced antibodies. We demonstrate a modular toolbox for rapid creation of synthetic IgGs, oligomerized antibodies, and antibodies with different tags or enzymatic functionalities and measure their performance in a variety of immunoassays. Furthermore, we demonstrate surface immobilization, high-throughput screening of antibody libraries, and rapid prototyping of antibodies based on modular antibody assembly.

scholarly journals Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

10.3791/51492 ◽  
2015 ◽  
Author(s):  
Shane Miersch ◽  
Zhijian Li ◽  
Rachel Hanna ◽  
Megan E. McLaughlin ◽  
Michael Hornsby ◽  
...  
Keyword(s):  
High Throughput ◽  
Synthetic Antibody ◽  
Antibody Libraries
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