scholarly journals Genetic Variants in the Transcriptional Regulatory Region of the ALOX5AP gene and Susceptibility to Ischemic Stroke in Chinese Populations

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dongzhi Yang ◽  
Xiangnan Huang ◽  
Chuanju Cui ◽  
Yuchao Zhang ◽  
Ya Li ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Genetic Variants ◽  
Regulatory Region ◽  
Chinese Populations ◽  
Transcriptional Regulatory ◽  
Transcriptional Regulatory Region ◽  
Alox5ap Gene

scholarly journals Transcriptional Regulation of gga-miR-451 by AhR:Arnt in Mycoplasma gallisepticum (HS Strain) Infection

2019 ◽  
Vol 20 (12) ◽  
pp. 3087 ◽  
Author(s):  
Yabo Zhao ◽  
Yali Fu ◽  
Yingfei Sun ◽  
Mengyun Zou ◽  
Xiuli Peng
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Chromatin Immunoprecipitation ◽  
Regulatory Region ◽  
Mycoplasma Gallisepticum ◽  
Luciferase Reporter ◽  
Binding Motif ◽  
Aryl Hydrocarbon ◽  
Transcriptional Regulatory ◽  
Reporter Assays ◽  
Transcriptional Regulatory Region

MicroRNAs (miRNAs) have been determined to be important regulators for pathogenic microorganism infection. However, it is largely unclear how miRNAs are triggered during pathogen infection. We previously reported that the up-regulation of gga-miR-451 negatively regulates the Mycoplasma gallisepticum (MG)-induced production of inflammatory cytokines via targeting tyrosine3-monooxygenase/tryptophan5-monooxygenase activation protein zeta (YWHAZ). The aim of this study was to investigate the mechanism regulating gga-miR-451 in MG infection in chickens. Analysis of gga-miR-451 precursor, pri-miR-451, and pre-miR-451 indicated that the regulation occurred transcriptionally. We also identified the transcriptional regulatory region of gga-miR-451 that contained consensus-binding motif for aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (Arnt) complex, which is known as the transcription factor that regulates gene expression. Luciferase reporter assays combined with chromatin immunoprecipitation (ChIP) demonstrated that AhR:Arnt bound directly to the promoter elements of gga-miR-451, which were responsible for gga-miR-451 transcription in the context of MG infection. Furthermore, upregulation of AhR:Arnt significantly induced gga-miR-451 and inhibited YWHAZ expression, suggesting that AhR:Arnt may play an anti-inflammatory role in MG infection. This discovery suggests that induced gga-miR-451 expression is modulated by AhR:Arnt in response to MG infection.

scholarly journals A Promoter Polymorphism (rs17222919, −1316T/G) of ALOX5AP Gene Is Associated with Decreased Risk of Ischemic Stroke in Two Independent Chinese Populations

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122393 ◽  
Author(s):  
Yujia Fan ◽  
Hui Chen ◽  
Aifan Li ◽  
Yunshu Shi ◽  
Yuchao Zhang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Promoter Polymorphism ◽  
Chinese Populations ◽  
Alox5ap Gene
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