scholarly journals Composition analysis of fractions of extracellular polymeric substances from an activated sludge culture and identification of dominant forces affecting microbial aggregation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xuan Guo ◽  
Xu Wang ◽  
Junxin Liu
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances ◽  
Composition Analysis

Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system

2013 ◽  
Vol 47 (14) ◽  
pp. 5298-5306 ◽  
Author(s):  
Juan Xu ◽  
Guo-Ping Sheng ◽  
Ying Ma ◽  
Long-Fei Wang ◽  
Han-Qing Yu
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances ◽  
Activated Sludge System

scholarly journals Isolation, Separation and Identification of the Extracellular Polymeric Substance (EPS) Protein Fraction from the Activated Sludge Floc

2021 ◽  
Author(s):  
Elena Brei
Keyword(s):  
Mass Spectrum ◽  
Activated Sludge ◽  
Extracellular Polymeric Substances ◽  
Current Knowledge ◽  
Noncovalent Interactions ◽  
Detection Methods ◽  
Structure Stability ◽  
Major Protein ◽  
Substantial Portion ◽  
Microbial Flocs

The purpose of this study was to expand the current knowledge of the composition of extracellular polymeric substances (EPS), principally EPS proteins, and their influence on structure, stability and surface chemistry of microbial flocs in activated sludge. It was proposed that a substantial portion of EPS proteins contains glycoproteins or proteins that are strongly bound noncovalently to carbohydrates. Various buffer additives, purification and precipitation methods were employed for protein purification and several glycoprotein detection methods were utilized for glycoprotein detection in the EPS. The proteins were separated with success, with a substantial portion suggesting either a possible glycosylation or strong noncovalent interactions with carbohydrate moiety. An enzyme, oligoendopeptidase F from M3B family was tentatively identified as a major protein present. These results suggest that proteins in the activated sludge EPS may exist in a very intricate arrangement. Furthermore, the EPS peptides may get degraded by naturally present enzymes in the EPS after the protein is digested prior to mass spectrum (MS), making the identification challenging.

A new method for extraction of extracellular polymeric substances from biofilms and activated sludge suitable for direct quantification of sorbed metals

2001 ◽  
Vol 43 (6) ◽  
pp. 25-31 ◽  
Author(s):  
S. Wuertz ◽  
R. Spaeth ◽  
A. Hinderberger ◽  
T. Griebe ◽  
H.-C. Flemming ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances ◽  
Microbial Population ◽  
Zinc Concentration ◽  
Extraction Procedure ◽  
Cation Exchange Resin ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Sorption Behavior ◽  
Gradual Change

A method for extraction of extracellular polymeric substances (EPSs) with a dicyclohexyl-18-crown-6 ether was developed to determine levels of organic and inorganic contaminants sorbed to EPS. The crown ether selectively binds alkaline and alkaline earth metals but not heavy metals. The effectiveness of the extraction procedure was higher than that of 2 other methods tested and comparable with that of a method based on a cation exchange resin. On average it was possible to extract 20% of the TOC, 12% of the total protein content, and 4% of the total carbohydrate content of sludge or biofilm biomass. Metal sorption studies in activated sludge showed no influence of exposure time on the fractionation of metals within the biomass. Metals sorbed mostly to cellular material. In biofilms 12.2% of the cadmium and 9.1% of the zinc added was found in the EPS. In activated sludge EPS contained only 2.9% zinc. The distribution of metals within the biomass was dose dependent. The percentage of metals found in EPS decreased with increasing metal concentration. This indicates a higher affinity of metals for cellular binding sites. Time course experiments in a rotating biofilm annular reactor, which consisted of an external cylinder with removable slides and an internal solid drum, revealed a gradual change in zinc concentration associated with EPS, although the total zinc concentration in the biomass remained constant. Concurrently, the amount of extractable EPS decreased. This was a consequence of a microbial population shift, with bacterial counts decreasing and algal and fungal biomass increasing. Using confocal laser scanning microscopy and the fluorescent metal complexing agent Newport Green for in situ detection of zinc it was shown that metals were bound to algae and fungi in the latter part of the experiment. The biofilm became more and more heterogeneous coinciding with a decrease in EPS. To summarize, the observed sorption behavior of metals cannot be explained with the conventional paradigm of EPS ashydrophilic gel. Obviously, different binding mechanisms must be invoked to explain the role of EPS in the sorption and removal of toxic substances in activated sludge and biofilm systems. It is important to consider the microbial population to understand differences in sorption in different matrices.

Characterization of interactions between a metabolic uncoupler O-chlorophenol and extracellular polymeric substances of activated sludge

2019 ◽  
Vol 247 ◽  
pp. 1020-1027 ◽  
Author(s):  
Fang Fang ◽  
Run-Ze Xu ◽  
Su-Na Wang ◽  
Lu-Lu Zhang ◽  
Yan-Qiu Huang ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances
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