scholarly journals Seven wood-inhabiting new species of the genus Trichoderma (Fungi, Ascomycota) in Viride clade

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Wen-Tao Qin ◽  
Wen-Ying Zhuang

Abstract More than 200 recent collections of Trichoderma from China were examined and 16 species belonging to the Viride clade were identified based on integrated studies of phenotypic and molecular data. Among them, seven wood-inhabiting new species, T. albofulvopsis, T. densum, T. laevisporum, T. sinokoningii, T. sparsum, T. sphaerosporum and T. subviride, are found. They form trichoderma- to verticillium-like conidiophores, lageniform to subulate phialides and globose to ellipsoidal conidia, but vary greatly in colony features, growth rates, and sizes of phialides and conidia. To explore their taxonomic positions, the phylogenetic tree including all the known species of the Viride clade is constructed based on sequence analyses of the combined RNA polymerase II subunit b and translation elongation factor 1 alpha exon genes. Our results indicated that the seven new species were well-located in the Koningii, Rogersonii and Neorufum subclades as well as a few independent terminal branches. They are clearly distinguishable from any existing species. Morphological distinctions and sequence divergences between the new species and their close relatives were discussed.

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.


Phytotaxa ◽  
2020 ◽  
Vol 470 (2) ◽  
pp. 133-144
Author(s):  
SI-PENG JIAN ◽  
RONG DAI ◽  
JUN GAO ◽  
BANG FENG

A new species, Cantharellus albus, was collected from southwestern China and described with both morphological and molecular characters. It differs from other species of Cantharellus by its smooth white pileus, white hymenophore, and white stipe, the latter turning yellow after bruising. The phylogenetic tree inferred from the combined dataset of three DNA fragments, namely large subunit ribosomal RNA gene (LSU), RNA polymerase II second largest subunit (rpb2), and translation elongation factor 1-alpha gene (tef1), indicated that the new species is distinct from other species of Cantharellus and is a member of sect. Flavobrunnei within the subgenus Parvocantharellus.


Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 28 ◽  
Author(s):  
KAI CHEN ◽  
WEN-YING ZHUANG

Seven new species of the genus Trichoderma belonging to the Viride clade are described from soil samples of different regions in China, and named as T. adaptatum, T. beijingense, T. bifurcatum, T. mangshanicum, T. shaoguanicum, T. tardum and T. vulgatum. Their phylogenetic positions are determined by analyses of the combined partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit encoding genes. Morphology and culture characteristics are observed, described and illustrated in detail. All the new species form green conidia, fit nicely the Viride clade, but differ obviously from any known species of the genus in sequence data, colony features, growth rates, and size of phialides and conidia. Distinctions between the new species and their close relatives are discussed.


Phytotaxa ◽  
2017 ◽  
Vol 305 (3) ◽  
pp. 121 ◽  
Author(s):  
WEN-TAO QIN ◽  
WEN-YING ZHUANG

Among 240 specimens of Trichoderma producing sexual states collected in different regions of China, 80 specimens representing 21 species possess green ascospores. Species were identified based on integrated studies of phenotypic features, culture characteristics and molecular analyses of RNA polymerase II subunit b and translation elongation factor 1-α genes. Of these 21 species, seven are recognized here as new: T. angustum, T. crystalligenum, T. globoides, T. perviride, T. purpureum, T. tenue and T. viridulum. Trichoderma angustum and T. crystalligenum belong to the Strictipile clade, and the remaining five species are in the Harzianum clade. Trichoderma angustum inhabits herbaceous stems and possesses trichoderma- to verticillium-like conidiophores. Trichoderma crystalligenum forms brownish yellow pulvinate stromata, pachybasium-like conidiophores, and abundant yellow crystals in culture. Trichoderma globoides and T. perviride share a common ancestor with but are different from T. alni, T. christiani, T. corneum and T. italicum. Trichoderma purpureum is a sister of T. epimyces, and is distinguished by its purplish red stromata. Trichoderma tenue is closely related to T. priciliae and T. rufobrunneum, and is characterized by verticillium-like conidiophores and slender phialides. Trichoderma viridulum is associated with T. cerinum, T. tomentosum and T. ceraceum; it is distinguishable by trichoderma-like rather than pachybasium-like conidiophores. Morphological distinctions between the new species and their allies are discussed. This study updates our knowledge of species diversity of the group.


2021 ◽  
Author(s):  
Debora Guterres ◽  
Roberto Ramos-Sobrinho ◽  
Danilo B. Pinho ◽  
Iraildes P. Assunção ◽  
Gaus S.A. Lima

Abstract Fungal species belonging to the genus Balansia (Clavicipitaceae) are well known as endophytic and epibiotic species commonly found on grasses or sedges. Among the 36 species of Balansia described worldwide, ten have been reported in Brazil. While most species of balansoid fungi were described on graminaceous plants, only four were characterized on cyperaceous hosts. To correctly identify the species of balansoid fungi associated with Scleria bracteata (Cyperaceae), specimens were collected in the state of Alagoas, Brazil, in 2014 and 2016. Nucleotide partial sequences of the second-largest subunit of RNA polymerase II (RPB2), translation elongation factor 1-α (TEF1), 18S subunit ribosomal DNA (SSU), 28S subunit ribosomal DNA (LSU), and internal transcribed spacers (ITS) were obtained from each balansoid specimen. Based on morphology and molecular data, the specimens were identified as a putative new species of Balansia, herein referred to as Balansia scleriae sp. nov.


Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 261 ◽  
Author(s):  
Liu Gao ◽  
Zhuoya Wei ◽  
Bo Wang ◽  
Mark L. Gleason ◽  
Rong Zhang ◽  
...  

Scleroramularia is a genus that includes species of the sooty blotch and flyspeck (SBFS) fungal complex that blemishes the surface of apple, pawpaw, and other fruit. In a survey of SBFS on banana (Musa basjoo), an isolate associated with the flyspeck mycelial type of SBFS was obtained from Zhanjiang, Guangdong, China. Based on analysis of morphology and phylogeny (the nuclear ribosomal internal transcribed space region and translation elongation factor 1-alpha), it was delimited as a new species of Scleroramularia, described here as S. musae. Conidia of S. musae have more septa than other species presently known in the genus.


Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).


Phytotaxa ◽  
2019 ◽  
Vol 415 (4) ◽  
pp. 179-188
Author(s):  
XIANG-NYU CHEN ◽  
MING ZHANG ◽  
TAI-HUI LI ◽  
NIAN-KAI ZENG

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.


MycoKeys ◽  
2019 ◽  
Vol 52 ◽  
pp. 71-87
Author(s):  
Sheng-Hua Wu ◽  
Chia-Ling Wei ◽  
Yu-Ting Lin ◽  
Chiung-Chih Chang ◽  
Shuang-Hui He

Four new species of Aleurodiscus sensu lato with echinulate basidiospores are described from East Asia: A.alpinus, A.pinicola, A.senticosus, and A.sichuanensis. Aleurodiscusalpinus is from northwest Yunnan of China where it occurs on Rhododendron in montane habitats. Aleurodiscuspinicola occurs on Pinus in montane settings in Taiwan and northwest Yunnan. Aleurodiscussenticosus is from subtropical Taiwan, where it occurs on angiosperms. Aleurodiscussichuanensis is reported from southwest China on angiosperms in montane environments. Phylogenetic relationships of these four new species were inferred from analyses of a combined dataset consisting of three genetic markers, viz. 28S, nuc rDNA ITS1-5.8S-ITS2 (ITS), and a portion of the translation elongation factor 1-alpha gene, TEF1.


Phytotaxa ◽  
2021 ◽  
Vol 512 (1) ◽  
Author(s):  
XIANGFU LIU ◽  
SAOWALUCK TIBPROMMA ◽  
FAN ZHANG ◽  
JIANCHU XU ◽  
K.W.T. CHETHANA ◽  
...  

In this paper we describe a new species of cave fungus belonging to Sporocadaceae (Amphisphaeriales), collected from Gem Cave, Fumin County, Yunnan Province, China. Initial morphological observations confirmed that our fungal collection is a pestalotioid species. Phylogenetic analyses of combined internal transcribed spacer (ITS), β-tubulin (TUB) and translation elongation factor 1-alpha (TEF1α) gene sequence dataset confirmed that our fungus forms an independent branch within Neopestalotiopsis. Thus, we describe our fungus as a new species of Neopestalotiopsis based on both morphology and multigene phylogeny. This is the first-ever report of Neopestalotiopsis from a cave habitat. A full description, micrographs and a phylogenetic tree showing the placement of the new species are provided.


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