scholarly journals Antibody validation and scoring guidelines for ABCG2 immunohistochemical staining in formalin-fixed paraffin-embedded colon cancer tissue

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Camilla Natasha Cederbye ◽  
Jesper Andreas Palshof ◽  
Tine Plato Hansen ◽  
Anne Katrine Duun-Henriksen ◽  
Dorte Linnemann ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Immunohistochemical Staining ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Antibody Validation ◽  
Formalin Fixed

scholarly journals Performance of Automated Dissection on Formalin-Fixed Paraffin-Embedded Tissue Sections for the 21-Gene Recurrence Score Assay

2020 ◽  
Vol 19 ◽  
pp. 153303382096076
Author(s):  
Peng Qi ◽  
Qian-ming Bai ◽  
Qian-lan Yao ◽  
Wen-tao Yang ◽  
Xiao-yan Zhou
Keyword(s):  
Ductal Carcinoma ◽  
Recurrence Score ◽  
Cancer Tissue ◽  
Tissue Block ◽  
Tissue Sections ◽  
Epithelial Tissues ◽  
Formalin Fixed Paraffin ◽  
Gene Assay ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

This study aimed to compare the performance of MilliSect dissection and manual dissection. Twenty-five formalin-fixed paraffin-embedded (FFPE) breast cancer tissue blocks were selected for comparison. Specific areas of interest (AOIs) in invasive carcinoma on tissue sections were transferred to dissection slides by manual macrodissection or the MilliSect instrument. The comparison criteria were 1) the time required for dissection; 2) RNA concentration and purity; 3) RNA quantity of 5 housekeeping genes (by RT-qPCR); and 4) ER, PR, HER2, Ki-67 and recurrence score (RS) values (by the 21-gene assay). Then, tumor-adjacent tissues, including fibrocollagenous and epithelial tissues, from the same selected tissue blocks of 8 of 25 patients were scraped using the mesodissection method, and their RS values were assessed to evaluate the influence of tumor-adjacent tissues on the target AOIs. Ultimately, 4 AOIs of invasive ductal carcinoma (IDC) from 1 tissue block of another 4 patients with lymph node (LN) metastases each, LN tissue and a mixture of IDC and LN tissue from the other tissue block of the same 4 patients were mesodissected to evaluate the influence of infiltrating lymphocyte levels on the RS values of AOIs. In our experience, the MilliSect instrument, which provides process management documentation, required more time than manual macrodissection (on average, approximately 9.1 min per sample versus 5.8 min per sample, respectively). The RNA yield and quality of the dissected tissues were comparable for the 2 methods. However, the tumor-adjacent tissues of the AOIs may influence the RS to some extent. Tumor-infiltrating lymphocytes (TILs) can dramatically increase RSs, far exceeding the influence of tumor-adjacent fibrocollagenous and epithelial tissues. In conclusion, MilliSect mesodissection is comparable to manual dissection. This mesodissection tool may facilitate AOI alignment and the dissection process for the 21-gene RS assay. Samples whose adjacent tissues are intermixed with TILs warrant special attention.

scholarly journals RNAscope in situ hybridization confirms mRNA integrity in formalin-fixed, paraffin-embedded cancer tissue samples

Oncotarget ◽  
2017 ◽  
Vol 8 (55) ◽  
pp. 93392-93403 ◽  
Author(s):  
Victoria Bingham ◽  
Leanne McIlreavey ◽  
Christine Greene ◽  
Edwina O’Doherty ◽  
Rebecca Clarke ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cancer Tissue ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Immunohistochemical Staining Patterns of Canine Meningiomas and Correlation with Published Immunophenotypes

2002 ◽  
Vol 39 (3) ◽  
pp. 311-321 ◽  
Author(s):  
K. F. Barnhart ◽  
J. Wojcieszyn ◽  
R. W. Storts
Keyword(s):  
Immunohistochemical Staining ◽  
Neuron Specific Enolase ◽  
Positive Staining ◽  
Neoplastic Cells ◽  
Vimentin Expression ◽  
Immunohistochemical Markers ◽  
Formalin Fixed Paraffin ◽  
Tumor Group ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

This study examined immunohistochemical staining patterns for several meningioma variants involving either the brain or spinal cord of dogs. Formalin-fixed, paraffin-embedded tissue from 15 tumors was obtained. The selected tumor group included seven meningothelial, three transitional, two malignant (anaplastic), one myxoid, one papillary, and one osteomatous meningiomas. Tumors were evaluated for reactivity to the following six immunohistochemical markers: vimentin, pancytokeratin, glial fibrillary acidic protein (GFAP), S100, neuron-specific enolase (NSE), and synaptophysin. Vimentin expression was detected in all meningiomas, and 14 of 15 tumors demonstrated intense vimentin staining in more than 50% of the neoplastic cells. Pancytokeratin expression was present in 11 of 15 neoplasms; however, positive staining frequently was focal and often involved a small percentage of the neoplastic cells. GFAP expression was detected in a single, anaplastic meningioma. Although expression of NSE and S100 was detected in 12 of 25 meningiomas, the intensity of the staining and the percentage of positive neoplastic cells was highly variable. Synaptophysin was uniformly negative. These results will help to establish immunohistochemical profiles for meningiomas that will improve our ability to correctly differentiate these neoplasms of meningeal origin from central nervous system tumors originating from other sites.

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