scholarly journals Solution structure and binding specificity of the p63 DNA binding domain

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Andreas Enthart ◽  
Christian Klein ◽  
Alexander Dehner ◽  
Murray Coles ◽  
Gerd Gemmecker ◽  
...  
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Binding Specificity ◽  
Binding Domain ◽  
Dna Binding Domain

Letter to Editor: Solution structure of the HPV-16 E2 DNA binding domain, a transcriptional regulator with a dimeric β-barrel fold

2004 ◽  
Vol 30 (2) ◽  
pp. 211-214 ◽  
Author(s):  
Alejandro D. Nadra ◽  
Tommaso Eliseo ◽  
Yu-Keung Mok ◽  
Fabio C.L. Almeida ◽  
Mark Bycroft ◽  
...  
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Transcriptional Regulator ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Hpv 16

scholarly journals Solution Structure of the DNA-Binding Domain of the Tomato Heat-Stress Transcription Factor HSF24

1996 ◽  
Vol 236 (3) ◽  
pp. 911-921 ◽  
Author(s):  
Jurgen Schultheiss ◽  
Olaf Kunert ◽  
Uwe Gase ◽  
Klaus-Dieter Scharf ◽  
Lutz Nover ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Heat Stress ◽  
Dna Binding ◽  
Solution Structure ◽  
Binding Domain ◽  
Dna Binding Domain

scholarly journals The C6 zinc finger and adjacent amino acids determine DNA-binding specificity and affinity in the yeast activator proteins LAC9 and PPR1.

1990 ◽  
Vol 10 (10) ◽  
pp. 5128-5137 ◽  
Author(s):  
M M Witte ◽  
R C Dickson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dna Binding ◽  
Zinc Finger ◽  
Binding Specificity ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Transcription Activator ◽  
Activator Proteins ◽  
Dna Binding Specificity

LAC9 is a DNA-binding protein that regulates transcription of the lactose-galactose regulon in Kluyveromyces lactis. The DNA-binding domain is composed of a zinc finger and nearby amino acids (M. M. Witte and R. C. Dickson, Mol. Cell. Biol. 8:3726-3733, 1988). The single zinc finger appears to be structurally related to the zinc finger of many other fungal transcription activator proteins that contain positively charged residues and six conserved cysteines with the general form Cys-Xaa2-Cys-Xaa6-Cys-Xaa6-9-Cys-Xaa2-Cys-Xaa 6-Cys, where Xaan indicates a stretch of the indicated number of any amino acids (R. M. Evans and S. M. Hollenberg, Cell 52:1-3, 1988). The function(s) of the zinc finger and other amino acids in DNA-binding remains unclear. To determine which portion of the LAC9 DNA-binding domain mediates sequence recognition, we replaced the C6 zinc finger, amino acids adjacent to the carboxyl side of the zinc finger, or both with the analogous region from the Saccharomyces cerevisiae PPR1 or LEU3 protein. A chimeric LAC9 protein, LAC9(PPR1 34-61), carrying only the PPR1 zinc finger, retained the DNA-binding specificity of LAC9. However, LAC9(PPR1 34-75), carrying the PPR1 zinc finger and 14 amino acids on the carboxyl side of the zinc finger, gained the DNA-binding specificity of PPR1, indicating that these 14 amino acids are necessary for specific DNA binding. Our data show that C6 fingers can substitute for each other and allow DNA binding, but binding affinity is reduced. Thus, in a qualitative sense C6 fingers perform a similar function(s). However, the high-affinity binding required by natural C6 finger proteins demands a unique C6 finger with a specific amino acid sequence. This requirement may reflect conformational constraints, including interactions between the C6 finger and the carboxyl-adjacent amino acids; alternatively or in addition, it may indicate that unique, nonconserved amino acid residues in zinc fingers make sequence-specifying or stabilizing contacts with DNA.

Solution structure of the Kluyveromyces lactis LAC9 Cd2Cys6 DNA-binding domain

1995 ◽  
Vol 2 (10) ◽  
pp. 898-905 ◽  
Author(s):  
Kevin H. Gardner ◽  
Stephen F. Anderson ◽  
Joseph E. Coleman
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Kluyveromyces Lactis ◽  
Binding Domain ◽  
Dna Binding Domain

Solution Structure of the DNA Binding Domain of HIV-1 Integrase

Biochemistry ◽  
1995 ◽  
Vol 34 (31) ◽  
pp. 9826-9833 ◽  
Author(s):  
Patricia J. Lodi ◽  
James A. Ernst ◽  
John Kuszewski ◽  
Alison B. Hickman ◽  
Alan Engelman ◽  
...  
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Hiv 1

Solution structure of the POU-specific DNA-binding domain of Oct-1

Nature ◽  
1993 ◽  
Vol 362 (6423) ◽  
pp. 852-855 ◽  
Author(s):  
Niek Dekker ◽  
Michel Cox ◽  
Rolf Boelens ◽  
C. Peter Verrijzer ◽  
Peter C. van der Vliet ◽  
...  
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Binding Domain ◽  
Dna Binding Domain

Refined solution structure of the c-terminal DNA-binding domain of human immunovirus-1 integrase

1999 ◽  
Vol 36 (4) ◽  
pp. 556-564 ◽  
Author(s):  
Astrid P.A.M. Eijkelenboom ◽  
Remco Sprangers ◽  
Karl H�rd ◽  
Ramon A. Puras Lutzke ◽  
Ronald H.A. Plasterk ◽  
...  
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
Binding Domain ◽  
Dna Binding Domain

Solution structure of the origin DNA-binding domain of SV40 T-antigen

1996 ◽  
Vol 3 (12) ◽  
pp. 1034-1039 ◽  
Author(s):  
Xuelian Luo ◽  
David G. Sanford ◽  
Peter A. Bullock ◽  
William W. Bachovchin
Keyword(s):  
Dna Binding ◽  
Solution Structure ◽  
T Antigen ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Sv40 T Antigen
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