scholarly journals Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
A. Odawara ◽  
H. Katoh ◽  
N. Matsuda ◽  
I. Suzuki
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Neuronal Networks ◽  
Induced Pluripotent Stem Cell ◽  
Drug Responses ◽  
Long Term Culture ◽  
Induced Pluripotent

Biophysical study of human induced Pluripotent Stem Cell-Derived cardiomyocyte structural maturation during long-term culture

2018 ◽  
Vol 499 (3) ◽  
pp. 611-617 ◽  
Author(s):  
Tiago P. Dias ◽  
Sandra N. Pinto ◽  
Juliana I. Santos ◽  
Tiago G. Fernandes ◽  
Fábio Fernandes ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Long Term Culture ◽  
Biophysical Study ◽  
Induced Pluripotent

scholarly journals Ultrastructural Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Long-Term Culture

2013 ◽  
Vol 77 (5) ◽  
pp. 1307-1314 ◽  
Author(s):  
Tsukasa Kamakura ◽  
Takeru Makiyama ◽  
Kenichi Sasaki ◽  
Yoshinori Yoshida ◽  
Yimin Wuriyanghai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Long Term Culture ◽  
Induced Pluripotent

scholarly journals Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats

2021 ◽  
Author(s):  
Jee Myung Yang ◽  
Sunho Chung ◽  
KyungA Yun ◽  
Bora Kim ◽  
Seongjun So ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Transplantation ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Histological Evaluation ◽  
Long Term Effects ◽  
Retinal Cells ◽  
Rpe Cells ◽  
Induced Pluripotent

AbstractRetinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE and photoreceptor for retinal regeneration has not yet been investigated. In this study, we evaluated the long-term effects of hiPSC-derived RPE and photoreceptor cell transplantation in Pde6b knockout rats to study RP; cells were injected into the subretinal space of the right eyes of rats before the appearance of signs of retinal degeneration at 2–3 weeks of age. Ten months after transplantation, we evaluated the cells using fundus photography, optical coherence tomography, and histological evaluation, and no abnormal cell proliferation was observed. A relatively large number of transplanted cells persisted during the first 4 months; subsequently, the number of these cells decreased gradually. Notably, immunohistochemical analysis revealed that the hiPSC-derived retinal cells showed characteristics of both RPE cells and photoreceptors of human origin after transplantation. Functional analysis of vision by scotopic electroretinogram revealed significant preservation of vision after transplantation. Our study suggests that the transplantation of hiPSC-derived retinal cells, including RPE cells and photoreceptors, has a potential therapeutic effect against irreversible retinal degenerative diseases.

Sign in / Sign up

Export Citation Format

Share Document