scholarly journals Erratum: Corrigendum: Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jeroen Vangindertael ◽  
Isabel Beets ◽  
Susana Rocha ◽  
Peter Dedecker ◽  
Liliane Schoofs ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Super Resolution ◽  
C Elegans ◽  
Resolution Mapping ◽  
Super Resolution Mapping

scholarly journals Super-resolution mapping of glutamate receptors in C. elegans by confocal correlated PALM

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Jeroen Vangindertael ◽  
Isabel Beets ◽  
Susana Rocha ◽  
Peter Dedecker ◽  
Liliane Schoofs ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Single Molecule ◽  
Genetic Regulation ◽  
Super Resolution ◽  
Receptor Expression ◽  
C Elegans ◽  
Resolution Mapping ◽  
Super Resolution Mapping ◽  
Receptor Clusters

Abstract Photoactivated localization microscopy (PALM) is a super-resolution imaging technique based on the detection and subsequent localization of single fluorescent molecules. PALM is therefore a powerful tool in resolving structures and putative interactions of biomolecules at the ultimate analytical detection limit. However, its limited imaging depth restricts PALM mostly to in vitro applications. Considering the additional need for anatomical context when imaging a multicellular organism, these limitations render the use of PALM in whole animals difficult. Here we integrated PALM with confocal microscopy for correlated imaging of the C. elegans nervous system, a technique we termed confocal correlated PALM (ccPALM). The neurons, lying below several tissue layers, could be visualized up to 10 μm deep inside the animal. By ccPALM, we visualized ionotropic glutamate receptor distributions in C. elegans with an accuracy of 20 nm, revealing super-resolution structure of receptor clusters that we mapped onto annotated neurons in the animal. Pivotal to our results was the TIRF-independent detection of single molecules, achieved by genetic regulation of labeled receptor expression and localization to effectively reduce the background fluorescence. By correlating PALM with confocal microscopy, this platform enables dissecting biological structures with single molecule resolution in the physiologically relevant context of whole animals.

Super resolution mapping of the near optical field and the gradient optical force

2013 ◽  
Author(s):  
Ryan M. Gelfand ◽  
Alireza Bonakdar ◽  
O. Gokalp Memis ◽  
Hooman Mohseni
Keyword(s):  
Super Resolution ◽  
Optical Field ◽  
Optical Force ◽  
Resolution Mapping ◽  
Super Resolution Mapping

scholarly journals Super-resolution Mapping of Enhanced Emission by Collective Plasmonic Resonances

ACS Nano ◽  
2019 ◽  
Vol 13 (4) ◽  
pp. 4514-4521 ◽  
Author(s):  
Ruben F. Hamans ◽  
Matteo Parente ◽  
Gabriel W. Castellanos ◽  
Mohammad Ramezani ◽  
Jaime Gómez Rivas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Resolution Mapping ◽  
Super Resolution Mapping

Super-Resolution Mapping of Light-Driven Reactions on Metal Nanostructures

2021 ◽  
Author(s):  
Simone Ezendam ◽  
Julian Gargiulo ◽  
Ana Sousa-Castillo ◽  
Lin Nan ◽  
Maximilian Maier ◽  
...  
Keyword(s):  
Super Resolution ◽  
Metal Nanostructures ◽  
Resolution Mapping ◽  
Super Resolution Mapping

Super-Resolution Mapping of Thin Gas Pockets

2003 ◽  
Author(s):  
H.G. Borgos ◽  
T. Randen ◽  
L. Sonneland
Keyword(s):  
Super Resolution ◽  
Resolution Mapping ◽  
Super Resolution Mapping
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