scholarly journals The feasibility of in vivo imaging of infiltrating blood cells for predicting the functional prognosis after spinal cord injury

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kazuya Yokota ◽  
Takeyuki Saito ◽  
Kazu Kobayakawa ◽  
Kensuke Kubota ◽  
Masamitsu Hara ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
In Vivo Imaging ◽  
Blood Cells ◽  
Cord Injury

scholarly journals Evaluation of Traumatic Spinal Cord Injury in a Rat Model Using 99mTc-GA-5 as a Potential In Vivo Tracer

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7138
Author(s):  
Vanessa Izquierdo-Sánchez ◽  
Pablo C. Zambrano-Rodríguez ◽  
Nadia Peña-Merino ◽  
Sirio Bolaños-Puchet ◽  
Horacio J. Reyes-Alva ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Monoclonal Antibody ◽  
Spinal Cord Injury ◽  
Molecular Imaging ◽  
In Vivo Imaging ◽  
Practical Method ◽  
Traumatic Spinal Cord Injury ◽  
Recognition Specificity ◽  
Cord Injury

Spinal cord injury (SCI) refers to the damage suffered in the spinal cord by any trauma or pathology. The purpose of this work was to determine whether 99mTc-GA-5, a radiotracer targeting Glial Fibrillary Acidic Protein (GFAP), can reveal in vivo the reactivation of astrocytes in a murine model with SCI. A method for the 99mTc radiolabeling of the mouse anti-GFAP monoclonal antibody GA-5 was implemented. Radiochemical characterization was performed, and radioimmunohistochemistry assays were used to evaluate the integrity of 99mTc-GA-5. MicroSPECT/CT was used for in vivo imaging to trace SCI in the rats. No alterations in the GA-5’s recognition/specificity ability were observed after the radiolabeling. The GA-5’s radiolabeling procedure implemented in this work offers a practical method to allow the in vivo following of this monoclonal antibody to evaluate its biodistribution and specificity for GFAP receptors using SPECT/CT molecular imaging.

In vivo imaging of engrafted neural stem cells: its application in evaluating the optimal timing of transplantation for spinal cord injury

2005 ◽  
Vol 19 (13) ◽  
pp. 1839-1841 ◽  
Author(s):  
Seiji Okada ◽  
Ken Ishii ◽  
Junichi Yamane ◽  
Akio Iwanami ◽  
Takeshi Ikegami ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Imaging ◽  
Optimal Timing ◽  
Cord Injury

scholarly journals In Vivo Imaging of Spinal Cord Injury

Neurosurgery ◽  
2005 ◽  
Vol 57 (1) ◽  
pp. N8-N8
Author(s):  
BRYAN E. FIGUEROA ◽  
ROBERT M. FRIEDLANDER
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
In Vivo Imaging ◽  
Cord Injury

scholarly journals In vivo imaging in experimental spinal cord injury – Techniques and trends

2021 ◽  
pp. 100859
Author(s):  
Vanessa Hubertus ◽  
Lea Meyer ◽  
Laurens Roolfs ◽  
Lilly Waldmann ◽  
Melina Nieminen-Kelhä ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
In Vivo Imaging ◽  
Experimental Spinal Cord Injury ◽  
Cord Injury

scholarly journals A Collagen-Based Scaffold for Promoting Neural Plasticity in a Rat Model of Spinal Cord Injury

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2245
Author(s):  
Jue-Zong Yeh ◽  
Ding-Han Wang ◽  
Juin-Hong Cherng ◽  
Yi-Wen Wang ◽  
Gang-Yi Fan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Rat Model ◽  
Neural Plasticity ◽  
Collagen Scaffold ◽  
Glial Scar ◽  
Beneficial Effects ◽  
Cord Injury

In spinal cord injury (SCI) therapy, glial scarring formed by activated astrocytes is a primary problem that needs to be solved to enhance axonal regeneration. In this study, we developed and used a collagen scaffold for glial scar replacement to create an appropriate environment in an SCI rat model and determined whether neural plasticity can be manipulated using this approach. We used four experimental groups, as follows: SCI-collagen scaffold, SCI control, normal spinal cord-collagen scaffold, and normal control. The collagen scaffold showed excellent in vitro and in vivo biocompatibility. Immunofluorescence staining revealed increased expression of neurofilament and fibronectin and reduced expression of glial fibrillary acidic protein and anti-chondroitin sulfate in the collagen scaffold-treated SCI rats at 1 and 4 weeks post-implantation compared with that in untreated SCI control. This indicates that the collagen scaffold implantation promoted neuronal survival and axonal growth within the injured site and prevented glial scar formation by controlling astrocyte production for their normal functioning. Our study highlights the feasibility of using the collagen scaffold in SCI repair. The collagen scaffold was found to exert beneficial effects on neuronal activity and may help in manipulating synaptic plasticity, implying its great potential for clinical application in SCI.

scholarly journals Immunosuppressants Affect Human Neural Stem Cells In Vitro but Not in an In Vivo Model of Spinal Cord Injury

2013 ◽  
Vol 2 (10) ◽  
pp. 731-744 ◽  
Author(s):  
Christopher J. Sontag ◽  
Hal X. Nguyen ◽  
Noriko Kamei ◽  
Nobuko Uchida ◽  
Aileen J. Anderson ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neural Stem Cells ◽  
In Vivo Model ◽  
Human Neural Stem Cells ◽  
Cord Injury

scholarly journals Depolarization and electrical stimulation enhance in vitro and in vivo sensory axon growth after spinal cord injury

2018 ◽  
Vol 300 ◽  
pp. 247-258 ◽  
Author(s):  
Ioana Goganau ◽  
Beatrice Sandner ◽  
Norbert Weidner ◽  
Karim Fouad ◽  
Armin Blesch
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Electrical Stimulation ◽  
Axon Growth ◽  
Sensory Axon ◽  
Cord Injury
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