scholarly journals Reduced expression of cytochrome oxidases largely explains cAMP inhibition of aerobic growth in Shewanella oneidensis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jianhua Yin ◽  
Qiu Meng ◽  
Huihui Fu ◽  
Haichun Gao
Keyword(s):  
Shewanella Oneidensis ◽  
Aerobic Growth ◽  
Cytochrome Oxidases

scholarly journals Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain

2008 ◽  
Vol 74 (24) ◽  
pp. 7561-7569 ◽  
Author(s):  
Vasiliy A. Portnoy ◽  
Markus J. Herrgård ◽  
Bernhard Ø. Palsson
Keyword(s):  
Escherichia Coli ◽  
Oxygen Uptake ◽  
Growth Conditions ◽  
Aerobic Growth ◽  
Acid Fermentation ◽  
Mixed Acid Fermentation ◽  
E Coli ◽  
Knockout Strain ◽  
Mixed Acid ◽  
Cytochrome Oxidases

ABSTRACT Fermentation of glucose to d-lactic acid under aerobic growth conditions by an evolved Escherichia coli mutant deficient in three terminal oxidases is reported in this work. Cytochrome oxidases (cydAB, cyoABCD, and cbdAB) were removed from the E. coli K12 MG1655 genome, resulting in the ECOM3 (E. coli cytochrome oxidase mutant) strain. Removal of cytochrome oxidases reduced the oxygen uptake rate of the knockout strain by nearly 85%. Moreover, the knockout strain was initially incapable of growing on M9 minimal medium. After the ECOM3 strain was subjected to adaptive evolution on glucose M9 medium for 60 days, a growth rate equivalent to that of anaerobic wild-type E. coli was achieved. Our findings demonstrate that three independently adaptively evolved ECOM3 populations acquired different phenotypes: one produced lactate as a sole fermentation product, while the other two strains exhibited a mixed-acid fermentation under oxic growth conditions with lactate remaining as the major product. The homofermenting strain showed a d-lactate yield of 0.8 g/g from glucose. Gene expression and in silico model-based analyses were employed to identify perturbed pathways and explain phenotypic behavior. Significant upregulation of ygiN and sodAB explains the remaining oxygen uptake that was observed in evolved ECOM3 strains. E. coli strains produced in this study showed the ability to produce lactate as a fermentation product from glucose and to undergo mixed-acid fermentation during aerobic growth.

Dynamic modeling of aerobic growth of Shewanella oneidensis. Predicting triauxic growth, flux distributions, and energy requirement for growth

2013 ◽  
Vol 15 ◽  
pp. 25-33 ◽  
Author(s):  
Hyun-Seob Song ◽  
Doraiswami Ramkrishna ◽  
Grigoriy E. Pinchuk ◽  
Alexander S. Beliaev ◽  
Allan E. Konopka ◽  
...  
Keyword(s):  
Dynamic Modeling ◽  
Energy Requirement ◽  
Shewanella Oneidensis ◽  
Aerobic Growth

scholarly journals Aerobic Respiration and Its Regulation in the Metal Reducer Shewanella oneidensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Kristen Bertling ◽  
Areen Banerjee ◽  
Daad Saffarini
Keyword(s):  
Carbon Sources ◽  
Shewanella Oneidensis ◽  
Anaerobic Respiration ◽  
Aerobic Respiration ◽  
Wild Type ◽  
Aerobic Growth ◽  
Camp Phosphodiesterase ◽  
Oxidase Gene ◽  
Oxygen Reductase ◽  
Mutant Phenotypes

Shewanella oneidensis MR-1 is a facultative anaerobe known for its ability to reduce metal oxides. Anaerobic respiration, especially metal reduction, has been the subject of extensive research. In contrast, S. oneidensis aerobic respiration has received less attention. S. oneidensis expresses cbb3- and aa3-type cytochrome c oxidases and a bd-type quinol oxidase. The aa3-type oxidase, which in other bacteria is the major oxygen reductase under oxygen replete conditions, does not appear to contribute to aerobic respiration and growth in S. oneidensis. Our results indicated that although the aa3-type oxidase does not play a role in aerobic growth on lactate, the preferred carbon source for S. oneidensis, it is involved in growth on pyruvate or acetate. These results highlight the importance of testing multiple carbon and energy sources when attempting to identify enzyme activities and mutant phenotypes. Several regulatory proteins contribute to the regulation of aerobic growth in S. oneidensis including CRP and ArcA. The 3',5'-cAMP phosphodiesterase (CpdA) appears to play a more significant role in aerobic growth than either CRP or ArcA, yet the deficiency does not appear to be the result of reduced oxidase genes expression. Interestingly, the ∆cpdA mutant was more deficient in aerobic respiration with several carbon sources tested compared to ∆crp, which was moderately deficient only in the presence of lactate. To identify the reason for ∆cpdA aerobic growth deficiency, we isolated a suppressor mutant with transposon insertion in SO_3550. Inactivation of this gene, which encodes an anti-sigma factor, restored aerobic growth in the cpdA mutant to wild-type levels. Inactivation of SO_3550 in wild-type cells, however, did not affect aerobic growth. The S. oneidensis genome encodes two additional CRP-like proteins that we designated CrpB and CrpC. Mutants that lack crpB and crpC were deficient in aerobic growth, but this deficiency was not due to the loss of oxidase gene expression.

Toxic Effects of Chromium(VI) on Anaerobic and Aerobic Growth of Shewanella oneidensis MR-1

2008 ◽  
Vol 20 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Sridhar Viamajala ◽  
Brent M. Peyton ◽  
Rajesh K. Sani ◽  
William A. Apel ◽  
James N. Petersen
Keyword(s):  
Shewanella Oneidensis ◽  
Toxic Effects ◽  
Aerobic Growth ◽  
Chromium Vi

scholarly journals Shewanella oneidensisMR-1 Utilizes both Sodium- and Proton-Pumping NADH Dehydrogenases during Aerobic Growth

2018 ◽  
Vol 84 (12) ◽  
Author(s):  
Kody L. Duhl ◽  
Nicholas M. Tefft ◽  
Michaela A. TerAvest
Keyword(s):  
Shewanella Oneidensis ◽  
Growth Yield ◽  
Growth Conditions ◽  
Proton Pumping ◽  
Sodium Ion ◽  
Aerobic Growth ◽  
Content Type ◽  
Growth Defects ◽  
Double Mutant Strain ◽  
Nadh Dehydrogenases

ABSTRACTShewanella oneidensisMR-1 is a metal-reducing bacterium with the ability to utilize many different terminal electron acceptors, including oxygen and solid-metal oxides. Both metal oxide reduction and aerobic respiration have been studied extensively in this organism. However, electron transport chain processes upstream of the terminal oxidoreductases have been relatively understudied in this organism, especially electron transfer from NADH to respiratory quinones. Genome annotation indicates thatS. oneidensisMR-1 encodes four NADH dehydrogenases, a proton-translocating dehydrogenase (Nuo), two sodium ion-translocating dehydrogenases (Nqr1 and Nqr2), and an “uncoupling” dehydrogenase (Ndh), but none of these complexes have been studied. Therefore, we conducted a study specifically focused on the effects of individual NADH dehydrogenase knockouts inS. oneidensisMR-1. We observed that two of the single-mutant strains, the ΔnuoNand ΔnqrF1mutants, exhibited significant growth defects compared with the wild type. However, the defects were minor and only apparent under certain growth conditions. Further testing of the ΔnuoNΔnqrF1double-mutant strain yielded no growth in minimal medium under oxic conditions, indicating that Nuo and Nqr1 have overlapping functions, but at least one is necessary for aerobic growth. Coutilization of proton- and sodium ion-dependent energetics has important implications for the growth of this organism in environments with varied pH and salinity, including microbial electrochemical systems.IMPORTANCEBacteria utilize a wide variety of metabolic pathways that allow them to take advantage of different energy sources, and to do so with varied efficiency. The efficiency of a metabolic process determines the growth yield of an organism, or the amount of biomass it produces per amount of substrate consumed. This parameter has important implications in biotechnology and wastewater treatment, where low growth yields are often preferred to minimize the production of microbial biomass. In this study, we investigated respiratory pathways containing NADH dehydrogenases with varied efficiency (i.e., the number of ions translocated per NADH oxidized) in the metal-reducing bacteriumShewanella oneidensisMR-1. We observed that two different respiratory pathways are used concurrently, and at least one pathway must be functional for growth under oxic conditions.

scholarly journals Shewanella oneidensis arcA Mutation Impairs Aerobic Growth Mainly by Compromising Translation

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 926
Author(s):  
Peilu Xie ◽  
Jiahao Wang ◽  
Huihui Liang ◽  
Haichun Gao
Keyword(s):  
Response Regulator ◽  
Shewanella Oneidensis ◽  
Growth Defect ◽  
Primary System ◽  
Translation Efficiency ◽  
Aerobic Growth ◽  
Regulatory Systems ◽  
Metabolic Transition ◽  
Ribosome Biosynthesis ◽  
Major Defect

Arc (anoxic redox control), one of the most intensely investigated two-component regulatory systems in γ-proteobacteria, plays a major role in mediating the metabolic transition from aerobiosis to anaerobiosis. In Shewanella oneidensis, a research model for respiratory versatility, Arc is crucial for aerobic growth. However, how this occurs remains largely unknown. In this study, we demonstrated that the loss of the response regulator ArcA distorts the correlation between transcription and translation by inhibiting the ribosome biosynthesis. This effect largely underlies the growth defect because it concurs with the effect of chloramphenicol, which impairs translation. Reduced transcription of ArcA-dependent ribosomal protein S1 appears to have a significant impact on ribosome assembly. We further show that the lowered translation efficiency is not accountable for the envelope defect, another major defect resulting from the ArcA loss. Overall, our results suggest that although the arcA mutation impairs growth through multi-fold complex impacts in physiology, the reduced translation efficacy appears to be a major cause for the phenotype, demonstrating that Arc is a primary system that coordinates proteomic resources with metabolism in S. oneidensis.

scholarly journals Transcriptional regulator ArcA mediates expression of oligopeptide transport systems both directly and indirectly in Shewanella oneidensis

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Huihui Liang ◽  
Yinting Mao ◽  
Yijuan Sun ◽  
Haichun Gao
Keyword(s):  
Response Regulator ◽  
Shewanella Oneidensis ◽  
Anaerobic Growth ◽  
Transport Systems ◽  
Peptide Transporter ◽  
Growth Defect ◽  
Aerobic Growth ◽  
Two Component System ◽  
Metabolic Transition ◽  
Peptide Uptake

Abstract In γ-proteobacterial species, such as Escherichia coli, the Arc (anoxic redox control) two-component system plays a major role in mediating the metabolic transition from aerobiosis to anaerobiosis, and thus is crucial for anaerobic growth but dispensable for aerobic growth. In Shewanella oneidensis, a bacterium renowned for respiratory versatility, Arc (SoArc) primarily affects aerobic growth. To date, how this occurs has remained largely unknown although the growth defect resulting from the loss of DNA-binding response regulator SoArcA is tryptone-dependent. In this study, we demonstrated that the growth defect is in part linked to utilization of oligopeptides and di-tripeptides, and peptide uptake but not peptide degradation is significantly affected by the SoArcA loss. A systematic characterization of major small peptide uptake systems manifests that ABC peptide transporter Sap and four proton-dependent oligopeptide transporters (POTs) are responsible for transport of oligopeptides and di-tripeptides respectively. Among them, Sap and DtpA (one of POTs) are responsive to the SoarcA mutation but only dtpA is under the direct control of SoArcA. We further showed that both Sap and DtpA, when overproduced, improve growth of the SoarcA mutant. While the data firmly establish a link between transport of oligopeptides and di-tripeptides and the SoarcA mutation, other yet-unidentified factors are implicated in the growth defect resulting from the SoArcA loss.

Exemplar Abstract for Shewanella oneidensis Venkateswaran et al. 1999.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Shewanella Oneidensis
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