scholarly journals Erratum: Corrigendum: Lipoteichoic acid of Streptococcus oralis Uo5: a novel biochemical structure comprising an unusual phosphorylcholine substitution pattern compared to Streptococcus pneumoniae

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Nicolas Gisch ◽  
Dominik Schwudke ◽  
Simone Thomsen ◽  
Nathalie Heß ◽  
Regine Hakenbeck ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Lipoteichoic Acid ◽  
Substitution Pattern ◽  
Streptococcus Oralis

scholarly journals Lipoteichoic acid of Streptococcus oralis Uo5: a novel biochemical structure comprising an unusual phosphorylcholine substitution pattern compared to Streptococcus pneumoniae

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Nicolas Gisch ◽  
Dominik Schwudke ◽  
Simone Thomsen ◽  
Nathalie Heß ◽  
Regine Hakenbeck ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Teichoic Acid ◽  
Structural Complexity ◽  
Lipoteichoic Acid ◽  
Close Relative ◽  
Structural Variations ◽  
Wall Teichoic Acid ◽  
Substitution Pattern ◽  
Type Iv ◽  
Streptococcus Oralis

Abstract Members of the Mitis group of streptococci possess teichoic acids (TAs) as integral components of their cell wall that are unique among Gram-positive bacteria. Both, lipoteichoic (LTA) and wall teichoic acid, are formed by the same biosynthetic pathway, are of high complexity and contain phosphorylcholine (P-Cho) residues. These residues serve as anchors for choline-binding proteins (CBPs), some of which have been identified as virulence factors of the human pathogen Streptococcus pneumoniae. We investigated the LTA structure of its close relative Streptococcus oralis. Our analysis revealed that S. oralis Uo5 LTA has an overall architecture similar to pneumococcal LTA (pnLTA) and can be considered as a subtype of type IV LTA. Its structural complexity is even higher than that of pnLTA and its composition differs in number and type of carbohydrate moieties, inter-residue connectivities and especially the P-Cho substitution pattern. Here, we report the occurrence of a saccharide moiety substituted with two P-Cho residues, which is unique as yet in bacterial derived surface carbohydrates. Finally, we could link the observed important structural variations between S. oralis and S. pneumoniae LTA to the divergent enzymatic repertoire for their TA biosynthesis.

scholarly journals R-roscovitine Reduces Lung Inflammation Induced by Lipoteichoic Acid and Streptococcus pneumoniae

2012 ◽  
Vol 18 (7) ◽  
pp. 1086-1095 ◽  
Author(s):  
Arie J. Hoogendijk ◽  
Joris J. T. H. Roelofs ◽  
JanWillem Duitman ◽  
Miriam H. P. van Lieshout ◽  
Dana C. Blok ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Lung Inflammation ◽  
Lipoteichoic Acid

scholarly journals Distribution and Genetic Diversity of the ABC Transporter Lipoproteins PiuA and PiaA within Streptococcus pneumoniae and Related Streptococci

2006 ◽  
Vol 188 (3) ◽  
pp. 1031-1038 ◽  
Author(s):  
Rachael H. Whalan ◽  
Simon G. P. Funnell ◽  
Lucas D. Bowler ◽  
Michael J. Hudson ◽  
Andrew Robinson ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Streptococcus Pneumoniae ◽  
Oral Streptococci ◽  
Pneumococcal Infections ◽  
Closely Related Species ◽  
Streptococcus Oralis ◽  
Amino Acid Divergence ◽  
Genes Encoding ◽  
Associated Proteins ◽  
Nucleotide Divergence

ABSTRACT Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. The existence of approximately 90 antigenically distinct capsular serotypes has greatly complicated the development of an effective pneumococcal vaccine. Virulence-associated proteins common and conserved among all capsular types now represent the best strategy to combat pneumococcal infections. PiuA and PiaA are the lipoprotein components of two pneumococcal iron ABC transporters and are required for full virulence in mouse models of infection. Here we describe a study of the distribution and genetic diversity of PiuA and PiaA within typical and atypical S. pneumoniae, Streptococcus oralis, and Streptococcus mitis strains. The genes encoding both PiuA and PiaA were present in all typical pneumococci tested, (covering 20 and 27 serotypes, respectively). The piuA gene was highly conserved within the typical pneumococci (0.3% nucleotide divergence), but was also present in “atypical” pneumococci and the closely related species S. mitis and S. oralis, showing up to 10.4% nucleotide divergence and 7.5% amino acid divergence from the typical pneumococcal alleles. Conversely, the piaA gene was found to be specific to typical pneumococci, 100% conserved, and absent from the oral streptococci, including isolates of S. mitis known to possess pneumolysin and autolysin. These are desirable qualities for a vaccine candidate and as a diagnostic tool for S. pneumoniae.

scholarly journals Unique poly(glycerophosphate) lipoteichoic acid and the glycolipids of a Streptococcus sp. closely related to Streptococcus pneumoniae

2000 ◽  
Vol 267 (17) ◽  
pp. 5520-5530 ◽  
Author(s):  
Peter Roethlisberger ◽  
Naoko Iida-Tanaka ◽  
Klaus Hollemeyer ◽  
Elmar Heinzle ◽  
Ineo Ishizuka ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Lipoteichoic Acid

scholarly journals Mutations in the tacF Gene of Clinical Strains and Laboratory Transformants of Streptococcus pneumoniae: Impact on Choline Auxotrophy and Growth Rate

2008 ◽  
Vol 190 (12) ◽  
pp. 4129-4138 ◽  
Author(s):  
Ana González ◽  
Daniel Llull ◽  
María Morales ◽  
Pedro García ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Repeat Unit ◽  
Teichoic Acid ◽  
Integral Membrane Protein ◽  
Nutritional Requirement ◽  
Spontaneous Mutant ◽  
Free Medium ◽  
Streptococcus Oralis ◽  
Complex Relationships ◽  
Lipoteichoic Acids

ABSTRACT The nutritional requirement that Streptococcus pneumoniae has for the aminoalcohol choline as a component of teichoic and lipoteichoic acids appears to be exclusive to this prokaryote. A mutation in the tacF gene, which putatively encodes an integral membrane protein (possibly, a teichoic acid repeat unit transporter), has been recently identified as responsible for generating a choline-independent phenotype of S. pneumoniae (M. Damjanovic, A. S. Kharat, A. Eberhardt, A. Tomasz, and W. Vollmer, J. Bacteriol. 189:7105-7111, 2007). We now report that Streptococcus mitis can grow in choline-free medium, as previously illustrated for Streptococcus oralis. While we confirmed the finding by Damjanovic et al. of the involvement of TacF in the choline dependence of the pneumococcus, the genetic transformation of S. pneumoniae R6 by using S. mitis SK598 DNA and several PCR-amplified tacF fragments suggested that a minimum of two mutations were required to confer improved fitness to choline-independent S. pneumoniae mutants. This conclusion is supported by sequencing results also reported here that indicate that a spontaneous mutant of S. pneumoniae (strain JY2190) able to proliferate in the absence of choline (or analogs) is also a double mutant for the tacF gene. Microscopic observations and competition experiments during the cocultivation of choline-independent strains confirmed that a minimum of two amino acid changes were required to confer improved fitness to choline-independent pneumococcal strains when growing in medium lacking any aminoalcohol. Our results suggest complex relationships among the different regions of the TacF teichoic acid repeat unit transporter.

scholarly journals Viridans Group Streptococci Are Donors in Horizontal Transfer of Topoisomerase IV Genes to Streptococcus pneumoniae

2003 ◽  
Vol 47 (7) ◽  
pp. 2072-2081 ◽  
Author(s):  
Luz Balsalobre ◽  
María José Ferrándiz ◽  
Josefina Liñares ◽  
Fe Tubau ◽  
Adela G. de la Campa
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleotide Sequence ◽  
Horizontal Transfer ◽  
Southern Blot Analysis ◽  
Fluoroquinolone Resistance ◽  
Topoisomerase Iv ◽  
Streptococcus Mitis ◽  
Streptococcus Oralis ◽  
Viridans Group Streptococci ◽  
Interspecific Recombination

ABSTRACT A total of 46 ciprofloxacin-resistant (Cipr) Streptococcus pneumoniae strains were isolated from 1991 to 2001 at the Hospital of Bellvitge. Five of these strains showed unexpectedly high rates of nucleotide variations in the quinolone resistance-determining regions (QRDRs) of their parC, parE, and gyrA genes. The nucleotide sequence of the full-length parC, parE, and gyrA genes of one of these isolates revealed a mosaic structure compatible with an interspecific recombination origin. Southern blot analysis and nucleotide sequence determinations showed the presence of an ant-like gene in the intergenic parE-parC regions of the S. pneumoniae Cipr isolates with high rates of variations in their parE and parC QRDRs. The ant-like gene was absent from typical S. pneumoniae strains, whereas it was present in the intergenic parE-parC regions of the viridans group streptococci (Streptococcus mitis and Streptococcus oralis). These results suggest that the viridans group streptococci are acting as donors in the horizontal transfer of fluoroquinolone resistance genes to S. pneumoniae.

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