scholarly journals Production of hepatitis E virus-like particles presenting multiple foreign epitopes by co-infection of recombinant baculoviruses

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ryoichi Shima ◽  
Tian Cheng Li ◽  
Yutaka Sendai ◽  
Chikako Kataoka ◽  
Yoshio Mori ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Baculoviruses ◽  
Virus Like Particles

scholarly journals Characterization of self-assembled virus-like particles of dromedary camel hepatitis e virus generated by recombinant baculoviruses

2015 ◽  
Vol 210 ◽  
pp. 8-17 ◽  
Author(s):  
Xianfeng Zhou ◽  
Michiyo Kataoka ◽  
Zheng Liu ◽  
Naokazu Takeda ◽  
Takaji Wakita ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Baculoviruses ◽  
Dromedary Camel ◽  
Virus Like Particles ◽  
Self Assembled

scholarly journals Immunogenicity and Antigenicity of Rabbit Hepatitis E Virus-Like Particles Produced by Recombinant Baculoviruses

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1573
Author(s):  
Huimin Bai ◽  
Michiyo Kataoka ◽  
Yasushi Ami ◽  
Yuriko Suzaki ◽  
Naokazu Takeda ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Vaccine Development ◽  
Ex Vivo ◽  
High Titer ◽  
Hepatitis E ◽  
Cross Reactivity ◽  
Recombinant Baculoviruses ◽  
Genotype 3 ◽  
Human Carcinoma ◽  
Virus Like Particles

Rabbit hepatitis E virus (HEV) is a novel HEV belonging to genotype 3 (HEV-3) in the Orthohepevirus A species of the genus Hepevirus, family Hepeviridae. Rabbit HEV was originally isolated from rabbits and found to cause zoonotic infection. Although rabbit HEV can be successfully grown in culture with several cell lines, including the human carcinoma cell line PLC/PRF/5, it is difficult to obtain the large amounts of viral antigen required for diagnosis and vaccine development. In this study, we expressed N-terminal 13 and 111 aa-truncated rabbit HEV ORF2 proteins using recombinant baculoviruses and obtained two types of virus-like particles (VLPs), RnVLPs and RsVLPs with ~35 and 24 nm diameter, respectively. Anti-rabbit HEV IgG antibodies were induced in high titer by immunizing rabbits with RnVLPs or RsVLPs. The antibody secretion in the serum persisted more than three years. RsVLPs showed stronger antigenic cross-reactivity against HEV-1, HEV-3 and HEV-4 than rat HEV. Moreover, anti-RsVLPs antibodies neutralized not only the cognate virus but also HEV-1, HEV-3 and HEV-4 ex vivo, indicating that rabbit HEV had the same serotype as human HEVs. In contrast, the antibody did not block rat HEV infection, demonstrating that rat HEV belonged to a different serotype. Animal experiments indicated that immunization with either RnVLPs or RsVLPs completely protected the rabbits from challenge by rabbit HEV, suggesting that the VLPs are candidates for rabbit HEV vaccine development.

scholarly journals Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Gergana Zahmanova ◽  
Milena Mazalovska ◽  
Katerina Takova ◽  
Valentina Toneva ◽  
Ivan Minkov ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nicotiana Benthamiana ◽  
Hepatitis E Virus ◽  
Chimeric Protein ◽  
Hepatitis E ◽  
Core Antigen ◽  
Efficient Production ◽  
Virus Like Particles ◽  
B Virus

The core antigen of hepatitis B virus (HBcAg) is capable of self-assembly into virus-like particles (VLPs) when expressed in a number of heterologous systems. Such VLPs are potential carriers of foreign antigenic sequences for vaccine design. In this study, we evaluated the production of chimeric HBcAg VLPs presenting a foreign epitope on their surface, the 551–607 amino acids (aa) immunological epitope of the ORF2 capsid protein of hepatitis E virus. A chimeric construct was made by the insertion of 56 aa into the immunodominant loop of the HBcAg. The sequences encoding the chimera were inserted into the pEAQ-HT vector and infiltrated into Nicotiana benthamiana leaves. The plant-expressed chimeric HBcHEV ORF2 551–607 protein was recognized by an anti-HBcAg mAb and anti-HEV IgG positive swine serum. Electron microscopy showed that plant-produced chimeric protein spontaneously assembled into “knobbly” ~34 nm diameter VLPs. This study shows that HBcAg is a promising carrier platform for the neutralizing epitopes of hepatitis E virus (HEV) and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.

scholarly journals Induction of antibody and interferon-γ production in mice immunized with virus-like particles of swine hepatitis E virus

2014 ◽  
Vol 15 (4) ◽  
pp. 575 ◽  
Author(s):  
Young-Jo Song ◽  
Woo-Jung Park ◽  
Seul-Kee Lee ◽  
Joong-Bok Lee ◽  
Seung-Yong Park ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Interferon Γ ◽  
Virus Like Particles ◽  
Swine Hepatitis

scholarly journals Chimeric Recombinant Hepatitis E Virus-like Particles as an Oral Vaccine Vehicle Presenting Foreign Epitopes

Virology ◽  
2002 ◽  
Vol 293 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Masahiro Niikura ◽  
Shiki Takamura ◽  
Gisen Kim ◽  
Satoru Kawai ◽  
Masayuki Saijo ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Oral Vaccine ◽  
Hepatitis E ◽  
Recombinant Hepatitis ◽  
Virus Like Particles

scholarly journals Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure

2009 ◽  
Vol 106 (31) ◽  
pp. 12986-12991 ◽  
Author(s):  
T. Yamashita ◽  
Y. Mori ◽  
N. Miyazaki ◽  
R. H. Cheng ◽  
M. Yoshimura ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles

Generation of hepatitis E virus-like particles of two new genotypes G5 and G6 and comparison of antigenic properties with those of known genotypes

2015 ◽  
Vol 178 (1-2) ◽  
pp. 150-157 ◽  
Author(s):  
Tian-Cheng Li ◽  
Michiyo Kataoka ◽  
Kazuaki Takahashi ◽  
Sayaka Yoshizaki ◽  
Takanobu Kato ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles ◽  
Antigenic Properties

scholarly journals Liver-specific Gene Delivery Using Engineered Virus-Like Particles of Hepatitis E Virus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Eun Byul Lee ◽  
Jung-Hee Kim ◽  
Wonhee Hur ◽  
Jung Eun Choi ◽  
Sung Min Kim ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Specific Gene ◽  
Virus Like Particles

The use of Bayesian methods for evaluating the performance of a virus-like particles-based ELISA for serology of hepatitis E virus infection in swine

2010 ◽  
Vol 163 (2) ◽  
pp. 329-335 ◽  
Author(s):  
Nicolas Rose ◽  
Annie Boutrouille ◽  
Christelle Fablet ◽  
François Madec ◽  
Marc Eloit ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bayesian Methods ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Like Particles
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