scholarly journals Tuning Liposome Membrane Permeability by Competitive Peptide Dimerization and Partitioning-Folding Interactions Regulated by Proteolytic Activity

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Seng Koon Lim ◽  
Camilla Sandén ◽  
Robert Selegård ◽  
Bo Liedberg ◽  
Daniel Aili
Keyword(s):  
Membrane Permeability ◽  
Proteolytic Activity ◽  
Liposome Membrane ◽  
Peptide Dimerization

scholarly journals STATE SYSTEM OF HEMOGLOBIN AND THE ACTIVITY OF ENZYMES OF HUMAN ERYTHROCYTES UNDER CONDITIONS OF HYPERGLYCEMIA IN VITRO

2017 ◽  
Vol 21 (10) ◽  
pp. 174-180
Author(s):  
A.A. Panarina ◽  
A.A. Pestryakova ◽  
A.V. Sadychova ◽  
N.A. Klenova ◽  
E.A. Lebedeva
Keyword(s):  
Red Blood Cells ◽  
Membrane Permeability ◽  
Proteolytic Activity ◽  
Blood Cells ◽  
Glycosylated Hemoglobin ◽  
Hemoglobin Level ◽  
Human Erythrocytes ◽  
Glycosylated Hemoglobin Level ◽  
Activity Of Enzymes

The study of condition of the system of hemoglobin, release of peptide com- pounds, and proteolytic activity of human erythrocytes under conditions of hy- perglycemia of varying degrees is carried out. The increase in the level of hy- perglycemia is accompanied by a decrease of affinity of hemoglobin to oxygen and the growth of level of metgemoglobin, the glycosylated hemoglobin level is increased in conditions of severe hyperglycemia. The level of formation of pep- tides in erythrocytes and the level of output of their cells is determined by the increase in trypsin-like activity in cytosol and increased membrane permeability of red blood cells.

Amino acid and peptide hemin derivatives as new promising virucidal agents

2012 ◽  
Vol 16 (03) ◽  
pp. 297-309 ◽  
Author(s):  
Sergei A. Okorochenkov ◽  
Galina A. Zheltukhina ◽  
Vitaly A. Roginsky ◽  
Nikolai N. Nossik ◽  
Sergei L. Zheltukhin ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Membrane Permeability ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Model System ◽  
Virucidal Activity ◽  
Liposome Membrane ◽  
Simplex Virus ◽  
Membrane Components ◽  
Modes Of Interaction

We synthesized a series of hemin derivatives (HDs) substituted by residues of amino acids and peptides at either one or two propionic-acid residues, and studied the virucidal activity of the compounds obtained against herpes simplex virus. Compounds 6,7-bis-(methyl ester N0 L-seryl)-protohemin (IX) (2) and 6,7-bis-[methyl ester N0-L-arginyl)-protohemin (IX) (6) shown the highest virucidal activity. We also investigated the interaction between HDs and lipid-membrane components as a possible mechanism of virucidal action. A model system including Clark's electrode and a micellar solution of methyl linoleate was used to quantitatively assess the capability of HDs to catalyze the oxidation of polyunsaturated fatty acids as components of lipid membranes. Another model system including liposomes that consisted of dioleoylphosphatydylcholine and was loaded with the fluorescent dye carboxyfluorescein was employed to examine the effect of HDs on lipid-membrane permeability. The kinetics and efficacy of increasing liposome-membrane permeability on exposure to HDs appeared to depend on the nature of the substituents in the HDs. The findings are strongly suggestive of the presence of two different modes of interaction between an HD and the lipid membrane, i.e. oxidative and non-oxidative mechanisms possibly underlie the virucidal action of HDs.

scholarly journals Study of the derivatives of 1-adamantane carboxylic acid to induced plateletaggregation human in vitro

2017 ◽  
Vol 21 (10) ◽  
pp. 143-150
Author(s):  
A.S. Gil’mutdinova ◽  
V.A. Ermokhin ◽  
N.A. Klenova ◽  
P.P. Purygin
Keyword(s):  
Carboxylic Acid ◽  
Red Blood Cells ◽  
Membrane Permeability ◽  
Proteolytic Activity ◽  
Blood Cells ◽  
Glycosylated Hemoglobin ◽  
Hemoglobin Level ◽  
Glycosylated Hemoglobin Level ◽  
Derivatives Of

The study of condition of the system of hemoglobin, release of peptide com- pounds, and proteolytic activity of human erythrocytes under conditions of hy- perglycemia of varying degrees is carried out. The increase in the level of hy- perglycemia is accompanied by a decrease of affinity of hemoglobin to oxygen and the growth of level of metgemoglobin, the glycosylated hemoglobin level is increased in conditions of severe hyperglycemia. The level of formation of pep- tides in erythrocytes and the level of output of their cells is determined by the increase in trypsin-like activity in cytosol and increased membrane permeability of red blood cells.

scholarly journals Tuning Liposome Membrane Permeability by Competitive Coiled Coil Heterodimerization and Heterodimer Exchange

Langmuir ◽  
2018 ◽  
Vol 34 (22) ◽  
pp. 6529-6537 ◽  
Author(s):  
Camilla Skyttner ◽  
Karin Enander ◽  
Christopher Aronsson ◽  
Daniel Aili
Keyword(s):  
Membrane Permeability ◽  
Coiled Coil ◽  
Liposome Membrane

Liposome Membrane Permeability Assay Using a Glucose Biosensor Flow Injection System

1995 ◽  
Vol 28 (9) ◽  
pp. 1571-1578 ◽  
Author(s):  
Takashi Katsu ◽  
Hongping Wei ◽  
Weiping Hu ◽  
Xiaomei Zhang ◽  
Xianen Zhang
Keyword(s):  
Membrane Permeability ◽  
Flow Injection ◽  
Glucose Biosensor ◽  
Injection System ◽  
Liposome Membrane ◽  
Flow Injection System ◽  
Permeability Assay

Initial Polymerization Sites Indicated During Mitochondrial Oxidation of Diaminobenzidine after Toluene Treatment

1977 ◽  
Vol 35 ◽  
pp. 408-409
Author(s):  
W. A. Shannon ◽  
M. A. Matlib
Keyword(s):  
Membrane Permeability ◽  
Cytochrome Oxidase ◽  
Rat Liver ◽  
Precise Definition ◽  
Cytochemical Localization ◽  
Oxidative Reaction ◽  
Mitochondrial Oxidation ◽  
Definition Of ◽  
Exogenous Substrates

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.

Sarcolemmal permeability changes during early myocardial anoxia

1978 ◽  
Vol 36 (2) ◽  
pp. 642-643
Author(s):  
M. Ashraf ◽  
L. Landa ◽  
L. Nimmo ◽  
C. M. Bloor
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Cell Membrane Permeability ◽  
Enzyme Release ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Sarcolemmal Permeability ◽  
Membrane Changes

Following coronary artery occlusion, the myocardial cells lose intracellular enzymes that appear in the serum 3 hrs later. By this time the cells in the ischemic zone have already undergone irreversible changes, and the cell membrane permeability is variably altered in the ischemic cells. At certain stages or intervals the cell membrane changes, allowing release of cytoplasmic enzymes. To correlate the changes in cell membrane permeability with the enzyme release, we used colloidal lanthanum (La+++) as a histological permeability marker in the isolated perfused hearts. The hearts removed from sprague-Dawley rats were perfused with standard Krebs-Henseleit medium gassed with 95% O2 + 5% CO2. The hypoxic medium contained mannitol instead of dextrose and was bubbled with 95% N2 + 5% CO2. The final osmolarity of the medium was 295 M osmol, pH 7. 4.

Sem Observations in Gastric Mucosa Exposed to Progressive Enzymatic Etching

1980 ◽  
Vol 38 ◽  
pp. 570-571
Author(s):  
C.A.E. Lemmi ◽  
D. Booth ◽  
G.E. Adomian
Keyword(s):  
Gastric Mucosa ◽  
Critical Point ◽  
Proteolytic Activity ◽  
Etching Process ◽  
Cellular Types

In order to enrich populations of homogeneous cellular types we dissociated gastric mucosa by enzymatic techniques. In addition, we used SEM to monitor the progressive etching of the mucosa. Two enzymes were tested: collagenase III with minimum proteolytic activity and Pronase with broader proteolytic effects. The gastric mucosa was exposed to the effect of the enzymes using everted stomach preparations. In this way the digestive action occured progressively from the lumen of the stomach toward the base of the glands. This “etching” process could be monitored conveniently by SEM. After incubation for periods varying from 30 to 210 minutes the tissues were stretched on dental wax, fixed in 2 % glutaralheyde, post-fixed in osmium, dehydrated, critical point dryed and coated with gold. A model MSM-5 “Mini-SEM” was used for observation. Gentle uncurling of the preparation before coating with gold produced fractures which revealed the structure of the gastric glandsin more detail.

Synthetic marine guanidines are effective antileishmanial compounds by altering the plasma membrane permeability

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
AG Tempone ◽  
LF Martins ◽  
EG Pinto ◽  
JT Mesquita ◽  
EL Bennett ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Permeability ◽  
Plasma Membrane Permeability
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