scholarly journals Serum microRNA microarray analysis identifies miR-4429 and miR-4689 are potential diagnostic biomarkers for biliary atresia

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Rui Dong ◽  
Zhen Shen ◽  
Chao Zheng ◽  
Gong Chen ◽  
Shan Zheng
Keyword(s):  
Biliary Atresia ◽  
Microarray Analysis ◽  
Diagnostic Biomarkers ◽  
Serum Microrna ◽  
Microrna Microarray

Abstract 442: Intralipid Protects the Heart in Late Pregnancy Against Ischemia/Reperfusion Injury by Reducing Cardiomyocyte Apoptosis via Mir122 Induction

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Jingyuan Li ◽  
Negar Motayagheni ◽  
Neusha Barakati ◽  
Mansoureh Eghbali
Keyword(s):  
Coronary Artery ◽  
Infarct Size ◽  
Reperfusion Injury ◽  
Microarray Analysis ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Late Pregnancy ◽  
Control Group ◽  
Myocardial Infarct Size ◽  
Microrna Microarray

The prevalence of coronary artery disease in late pregnancy (LP) has increased recently due to significant changes in women’s lifestyle patterns (age, stress, smoking, diabetes and chronic hypertension). Myocardial infarction during LP and the peripartum is associated with significant maternal mortality and morbidity compared to non pregnant women for unclear reasons. We have recently demonstrated that cardiac vulnerability to I/R injury drastically increases in LP rodents, leading to myocardial infarct size ~4 fold greater than in non-pregnant controls. We also discovered that administration of intralipid (an emulsion of soy bean oil, egg yolk phospholipids and glycerol) at reperfusion resulted in ~60% reduction in infarct size of the heart in LP rat subjected to I/R injury. However, the molecular mechanisms underlying intralipid-induced cardioprotection in late pregnancy is not clear. Here we hypothesized that intralipid protects the heart in late pregnancy by regulating the levels of specific microRNAs. The left anterior descending coronary artery was occluded in LP rats (21-22 days of pregnancy) for 45 min followed by 3 hr of reperfusion. One single bolus of PBS (control group) or 20% intralipid (intralipid group) was applied through the femoral vein 5 min before the reperfusion. The hearts of control and intralipid groups were used for microRNA microarray analysis (Ocean Ridge Biosciences). MicroRNA-microarray analysis identified MiR122 as a novel micro-RNA which its expression was strikingly upregulated more than 10 fold in the heart of LP rats in intralipid group compared to control group. miR122 regulates apoptosis in cardiomyocytes subjected to hypoxia/reoxygenation since miR122-overexpression resulted in reduced apoptosis, whereas knockdown of miR122 enhanced apoptosis. Pyruvate kinase isoform M2 (PKM2), which is known to regulate cell apoptosis in the liver, is a direct target of miR122. Our data show that PKM2 and caspase 3 are two targets of miR122 since the expression of PKM2 and capase-3 in the heats subjected to I/R was significantly lower in intralipid group compared to control group in LP. In conclusion intralipid protects the heart in late pregnancy against ischemia/reperfusion injury via inducing miR122 by targeting PKM2.

scholarly journals Serum microRNA expression profiling revealing potential diagnostic biomarkers for lung adenocarcinoma

2020 ◽  
Vol 133 (21) ◽  
pp. 2532-2542
Author(s):  
Xia Shan ◽  
Lan Zhang ◽  
Dan-Xia Zhu ◽  
Xin Zhou ◽  
Huo Zhang ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Expression Profiling ◽  
Microrna Expression ◽  
Diagnostic Biomarkers ◽  
Serum Microrna

scholarly journals Expression Profile of Long Noncoding RNAs in Human Earlobe Keloids: A Microarray Analysis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Liang Guo ◽  
Kai Xu ◽  
Hongbo Yan ◽  
Haifeng Feng ◽  
Linlin Chai ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Expression Profiles ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Diagnostic Biomarkers ◽  
Pathway Enrichment ◽  
Normal Tissues ◽  
Wide Range ◽  
Skin Scar

Background. Long noncoding RNAs (lncRNAs) play key roles in a wide range of biological processes and their deregulation results in human disease, including keloids. Earlobe keloid is a type of pathological skin scar, and the molecular pathogenesis of this disease remains largely unknown. Methods. In this study, microarray analysis was used to determine the expression profiles of lncRNAs and mRNAs between 3 pairs of earlobe keloid and normal specimens. Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to identify the main functions of the differentially expressed genes and earlobe keloid-related pathways. Results. A total of 2068 lncRNAs and 1511 mRNAs were differentially expressed between earlobe keloid and normal tissues. Among them, 1290 lncRNAs and 1092 mRNAs were upregulated, and 778 lncRNAs and 419 mRNAs were downregulated. Pathway analysis revealed that 24 pathways were correlated to the upregulated transcripts, while 11 pathways were associated with the downregulated transcripts. Conclusion. We characterized the expression profiles of lncRNA and mRNA in earlobe keloids and suggest that lncRNAs may serve as diagnostic biomarkers for the therapy of earlobe keloid.

MicroRNA Microarray Analysis to Investigate the Key Genes and microRNAs Regulated by Amomum cardamomum in Nephropathy Rat Model

2018 ◽  
Vol 14 (3) ◽  
pp. 310-319
Author(s):  
M.M. Hongmei Chen ◽  
M.D. Xiulan Wang ◽  
M.M. Enhesuren ◽  
B.M. Chang Chun ◽  
B.M. Wenjie Jin ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Rat Model ◽  
Key Genes ◽  
Microrna Microarray

scholarly journals A Signature of Four Circulating microRNAs as Potential Biomarkers for Diagnosing Early-Stage Breast Cancer

2021 ◽  
Vol 22 (11) ◽  
pp. 6121
Author(s):  
Maha M. Itani ◽  
Farah J. Nassar ◽  
Arafat H. Tfayli ◽  
Rabih S. Talhouk ◽  
Ghada K. Chamandi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Diagnostic Accuracy ◽  
Ductal Carcinoma ◽  
Early Stage ◽  
Healthy Controls ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Predominant Type ◽  
New Biomarkers ◽  
Microrna Microarray

Breast cancer (BC) is the most predominant type of cancer among women. The aim of this study is to find new biomarkers that can help in early detection of BC, especially for those who are too young to be screened using mammography as per guidelines. Using microRNA microarray, we previously showed dysregulation of 74 microRNAs in tumors from early BC patients as compared with normal adjacent tissues, which we were interested in studying in blood circulation. In this study, we investigated the expression of 12 microRNA (miR-21/miR-155/miR-23a/miR-130a/miR-145/miR-425-5p/miR-139-5p/miR-451/miR-195/miR-125b/miR-100, and miR-182) in the plasma of 41 newly diagnosed Lebanese BC patients with early invasive ductal carcinoma as compared with 32 healthy controls. Total RNA was extracted from plasma, and expression levels of miRNA of interest were measured using RT-qPCR followed by statistical analysis; miR-21, miR-155, miR-23a, miR-130a, miR-145, miR-425-5p, and miR-139-5p were significantly upregulated and miR-451 was significantly downregulated, in the plasma of BC patients as compared with healthy controls. The positively correlated miR-23a, miR-21, and miR-130a had a high diagnostic accuracy (86%). Importantly, the combination of miR-145/miR-425-5p/miR-139-5p/miR-130a scored the highest diagnostic accuracy of 95% with AUC = 0.97 (sensitivity 97% and specificity 91%). MicroRNAs are promising non-invasive diagnostic biomarkers for early-stage BC with the panel of miR-145/miR-425-5p/miR-139-5p/miR-130a having the highest diagnostic accuracy.

scholarly journals Long Noncoding RNA GUSBP3 And SAM4 Function As Potential Diagnostic Biomarkers of Type 2 Diabetes Mellitus In Kazak Populations In The Xinjiang Region of China

2021 ◽  
Author(s):  
Tao Ba ◽  
Tianshui Ma ◽  
Yuan Sun ◽  
YuRong Zhu ◽  
Xiaoli Wang ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Real Time ◽  
Microarray Analysis ◽  
Logistic Regression Analysis ◽  
Differentially Expressed ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Diagnostic Biomarkers ◽  
Kazakh Population

Abstract Background: The pathogenesis of T2DM is influenced by a combination of genetic and environmental factors, among which LncRNAs have a huge impact on diabetes.In this study, we investigated the diagnostic value of lncRNA GUSBP3 and lncRNA SAM4 for T2DM in Kazakhs in Xinjiang. Methods: In this study, differentially expressed lncRNAs and mRNAs were screened by microarray analysis microarray in a Kazakh population in Xinjiang, and the expression of two candidate lncRNAs (lnc-GUSBP-3 and lnc-SAM-4) was further validated by quantitative real-time polymerization chain reaction (qRT-PCR).Meanwhile, GO (Gene Ontology) enrichment analysis were used to reveal the biological functions of the two candidate lncRNAs. Spearman’s correlation analysis was used to elucidate the correlation between lnc-GUSBP-3 and lnc-SAM-4 expression and metabolic characteristics. We found that the expression of lnc-GUSBP-3 and lnc-SAM-4 was up-regulated in the T2DM group compared to healthy controls. Multivariate logistic regression analysis showed that DBP, FIns and lnc-GUSBP3 were associated with T2DM susceptibility.In addition, ROC curves were used to evaluate the diagnostic potential of lnc-GUSBP3 and lnc-SAM4. Results: We conducted a microarray analysis of PBMC collected from patients with T2DM and healthy controls, all of Kazakh origin. In the microanalysis, we identified 89 differentially expressed lncRNAs, and 147 differentially expressed mRNAs. real-time quantitative reverse transcription polymerase chain reaction analysis of peripheral blood mononuclear cell (PBMC) samples from T2DM patients and healthy controls from the Xinjiang Kazakh population revealed significantly increased levels of lncRNAs GUSBP3 and SAM4 in T2DM patients. Logistic regression analysis revealed that lncRNA GUSBP3 expression correlated negatively with FIns, but positively with creatinine and uric acid (UA). Furthermore, lncRNA SAM4 expression correlated negatively with low-density lipoprotein cholesterol levels, but positively with UA. The area under the receiver operating characteristic curve values for lnc-GUSBP3 and lnc-SAM4 were 0.789 (95% CI = 0.672–0.906) and 0.741 (95% CI = 0.616–0.866), respectively. Conclusion: There were significant changes in lncRNA and mRNA in Kazakh T2DM patients. LncRNA-GUSBP3 and lncRNA-SAM4 may serve as potential diagnostic biomarkers for T2DM in Kazakhs in Xinjiang.

QS130. Microrna Microarray Analysis of Human Adrenocortical Tumors

2009 ◽  
Vol 151 (2) ◽  
pp. 288
Author(s):  
P.S. Soon ◽  
L. Tacon ◽  
M.S. Sywak ◽  
P. Campbell ◽  
M.W. Yeh ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Adrenocortical Tumors ◽  
Microrna Microarray
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