scholarly journals An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shreelatha Bhat ◽  
Walton D. Jones
Keyword(s):  
Odorant Receptor ◽  
Receptor Expression

The corepressor Atrophin specifies odorant receptor expression in Drosophila

2013 ◽  
Vol 28 (3) ◽  
pp. 1355-1364 ◽  
Author(s):  
Liza Alkhori ◽  
Anita Öst ◽  
Mattias Alenius
Keyword(s):  
Odorant Receptor ◽  
Receptor Expression

scholarly journals Deletion of voltage-gated channel affects glomerular refinement and odorant receptor expression in the mouse olfactory system

2007 ◽  
Vol 506 (2) ◽  
pp. 161-179 ◽  
Author(s):  
K.C. Biju ◽  
David Ronald Marks ◽  
Thomas Gerald Mast ◽  
Debra Ann Fadool
Keyword(s):  
Olfactory System ◽  
Odorant Receptor ◽  
Receptor Expression ◽  
Voltage Gated ◽  
Gated Channel

scholarly journals Advantage of the Highly Restricted Odorant Receptor Expression Pattern in Chemosensory Neurons of Drosophila

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66173 ◽  
Author(s):  
Sana Khalid Tharadra ◽  
Adriana Medina ◽  
Anandasankar Ray
Keyword(s):  
Expression Pattern ◽  
Odorant Receptor ◽  
Receptor Expression ◽  
Chemosensory Neurons

scholarly journals A Feedback Mechanism Regulates Odorant Receptor Expression in the Malaria Mosquito, Anopheles gambiae

2020 ◽  
Author(s):  
Sarah E. Maguire ◽  
Ali Afify ◽  
Loyal A. Goff ◽  
Christopher J. Potter
Keyword(s):  
Drosophila Melanogaster ◽  
Anopheles Gambiae ◽  
Odorant Receptor ◽  
Ectopic Expression ◽  
Feedback Mechanism ◽  
Receptor Expression ◽  
Rna Seq ◽  
Human Odor ◽  
Mosquito Anopheles Gambiae ◽  
Almost All

ABSTRACTMosquitoes locate and approach humans (‘host-seek’) when specific Olfactory Neurons (ORNs) in the olfactory periphery activate a specific combination of glomeruli in the mosquito Antennal Lobe (AL). We hypothesize that dysregulating proper glomerular activation in the presence of human odor will prevent host-seeking behavior. In experiments aimed at ectopically activating most ORNs in the presence of human odor, we made a surprising finding: ectopic expression of an AgOr (AgOr2) in Anopheles gambiae ORNs dampens the activity of the expressing neuron. This contrasts studies in Drosophila melanogaster, the typical insect model of olfaction, in which ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. To gain insight into this dysfunction in mosquitoes, RNA-seq analyses were performed comparing wild-type antennae to those ectopically expressing AgOr2 in ORNs. Remarkably, almost all Or transcripts were significantly downregulated (except for AgOr2), and additional experiments suggest that it is AgOR2 protein rather than mRNA that mediates this downregulation. Our study shows that ORNs of Anopheles mosquitoes (in contrast to Drosophila) employ a currently unexplored regulatory mechanism of OR expression, which may be adaptable as a vector-control strategy.SIGNIFICANCE STATEMENTStudies in Drosophila melanogaster suggest that insect Olfactory Receptor Neurons (ORNs) do not contain mechanisms by which Odorant Receptors (ORs) regulate OR expression. This has proved useful in studies where ectopic expression of an OR in Drosophila ORNs confers responses to the odorants that activate the newly expressed OR. In experiments in Anopheles gambiae mosquitoes, we found that ectopic expression of an OR in most Anopheles ORNs dampened the activity of the expressing neurons. RNA-seq analyses demonstrated that ectopic OR expression in Anopheles ORNs leads to downregulation of endogenous Or transcripts. Additional experiments suggest that this downregulation required ectopic expression of a functional OR protein. These findings reveal that Anopheles mosquitoes, in contrast to Drosophila, contain a feedback mechanism to regulate OR expression. Mosquito ORNs might employ regulatory mechanisms of OR expression previously thought to occur only in non-insect olfactory systems.

scholarly journals Innate immune signaling in the olfactory epithelium reduces odorant receptor levels: modeling transient smell loss in COVID-19 patients

2020 ◽  
Author(s):  
Steve Rodriguez ◽  
Luxiang Cao ◽  
Gregory T. Rickenbacher ◽  
Eric G. Benz ◽  
Colin Magdamo ◽  
...  
Keyword(s):  
Olfactory Epithelium ◽  
Odorant Receptor ◽  
Recovery Phase ◽  
Odor Discrimination ◽  
Olfactory Function ◽  
Receptor Expression ◽  
Odorant Receptors ◽  
Type I ◽  
Innate Immune Signaling ◽  
Leading Symptom

Post-infectious anosmias typically follow death of olfactory sensory neurons (OSNs) with a months-long recovery phase associated with parosmias. While profound anosmia is the leading symptom associated with COVID-19 infection, many patients regain olfactory function within days to weeks without distortions. Here, we demonstrate that sterile induction of anti-viral type I interferon signaling in the mouse olfactory epithelium is associated with diminished odor discrimination and reduced odor-evoked local field potentials. RNA levels of all class I, class II, and TAAR odorant receptors are markedly reduced in OSNs in a non-cell autonomous manner. We find that people infected with COVID-19 rate odors with lower intensities and have odor discrimination deficits relative to people that tested negative for COVID-19. Taken together, we propose that inflammatory-mediated loss of odorant receptor expression with preserved circuit integrity accounts for the profound anosmia and rapid recovery of olfactory function without parosmias caused by COVID-19.

scholarly journals Odorant Receptor Expression Defines Functional Units in the Mouse Olfactory System

2002 ◽  
Vol 22 (8) ◽  
pp. 3033-3043 ◽  
Author(s):  
Thomas Bozza ◽  
Paul Feinstein ◽  
Chen Zheng ◽  
Peter Mombaerts
Keyword(s):  
Olfactory System ◽  
Odorant Receptor ◽  
Receptor Expression ◽  
Functional Units

scholarly journals Lateral Signaling Mediated by Axon Contact and Calcium Entry Regulates Asymmetric Odorant Receptor Expression in C. elegans

Cell ◽  
1999 ◽  
Vol 99 (4) ◽  
pp. 387-398 ◽  
Author(s):  
Emily R. Troemel ◽  
Alvaro Sagasti ◽  
Cornelia I. Bargmann
Keyword(s):  
Odorant Receptor ◽  
Calcium Entry ◽  
Receptor Expression ◽  
C Elegans
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