scholarly journals Very-low and low-density lipoproteins induce neutral lipid accumulation and impair migration in monocyte subsets

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
William D. Jackson ◽  
Tobias W. Weinrich ◽  
Kevin J. Woollard
Keyword(s):  
Neutral Lipid ◽  
Lipid Accumulation ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Monocyte Subsets

scholarly journals Effect of Exposure of Human Monocyte-Derived Macrophages to High, versus Normal, Glucose on Subsequent Lipid Accumulation from Glycated and Acetylated Low-Density Lipoproteins

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Fatemeh Moheimani ◽  
Joanne T. M. Tan ◽  
Bronwyn E. Brown ◽  
Alison K. Heather ◽  
David M. van Reyk ◽  
...  
Keyword(s):  
High Glucose ◽  
Lipid Accumulation ◽  
Scavenger Receptor ◽  
Ldl Receptor ◽  
Human Monocyte ◽  
Low Density Lipoproteins ◽  
Receptor Expression ◽  
Low Density ◽  
Cholesteryl Esters ◽  
Protein Levels

During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. Here we examined the hypothesis that hyperglycaemia may modulate receptor expression and hence lipid accumulation in macrophages. Human monocytes were matured into macrophages in 30 versus 5 mM glucose and receptor expression and lipid accumulation quantified. High glucose elevated LOX1 mRNA, but decreased SR-AI, SR-BI, LDLR, and CD36 mRNA. SR-BI and CD36 protein levels were decreased. Normo- and hyperglycaemic cells accumulated cholesteryl esters from modified LDL to a greater extent than control LDL, but total and individual cholesteryl ester accumulation was not affected by glucose levels. It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined.

The release of lipoproteins by rat liver slices

1969 ◽  
Vol 47 (1) ◽  
pp. 65-69 ◽  
Author(s):  
A. I. Kook ◽  
D. Rubinstein
Keyword(s):  
Fatty Acids ◽  
Neutral Lipid ◽  
Incubation Medium ◽  
Ringer Solution ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Liver Slices ◽  
Lipid Moiety ◽  
Protein Moiety

The release of the lipid and protein moieties of lipoproteins by liver slices from rats charged in vivo with palmitate-9,10-3H and leucine-1-14C was followed. The replacement of Ringer solution as the incubation medium by serum resulted in an increase in the release of the labelled lipid moiety but had no effect on the radioactivity of the protein moiety. Analysis of the lipoproteins indicated that serum had no effect on the radioactivity of the neutral lipid in the lipoprotein of d < 1.063 or β-lipoproteins prepared by ultracentrifugation or heparin precipitation, respectively. However, the lipoprotein of d < 1.063 released into serum contained considerable amounts of labelled phospholipids and fatty acids which were not present in the β-lipoprotein precipitated by heparin or in the low density lipoproteins prepared by either procedure following incubation in Ringer solution. Following incubation of the slices in serum, but not in Ringer solution, the lipoprotein having d > 1.063 and the supernatant of the heparin precipitate contained considerable amounts of labelled phospholipid. It is concluded that serum may contain a lipoprotein capable of combining with liver phospholipids and that ultracentrifugation results in the separation of low density lipoproteins containing excess lipid.

Atherogenic diets and neutral-lipid organization in plasma low density lipoproteins

1979 ◽  
Vol 33 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Tomas Kirchhausen ◽  
Steven H. Untracht ◽  
Gunther M. Fless ◽  
Angelo M. Scanu
Keyword(s):  
Neutral Lipid ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Lipid Organization
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