scholarly journals PtrWRKY19, a novel WRKY transcription factor, contributes to the regulation of pith secondary wall formation in Populus trichocarpa

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Li Yang ◽  
Xin Zhao ◽  
Fan Yang ◽  
Di Fan ◽  
Yuanzhong Jiang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Secondary Wall ◽  
Populus Trichocarpa ◽  
Wrky Transcription Factor ◽  
Wall Formation ◽  
Secondary Wall Formation

scholarly journals Genome-wide identification of poplar malectin/malectin-like domain-containing proteins and in-silico expression analyses find novel candidates for signaling and regulation of wood development

2020 ◽  
Author(s):  
Vikash Kumar ◽  
Félix Barbut ◽  
Sunita Kushwah ◽  
Evgeniy N. Donev ◽  
János Urbancsok ◽  
...  
Keyword(s):  
In Silico ◽  
Secondary Wall ◽  
Populus Trichocarpa ◽  
Protein Domain ◽  
Protein Motif ◽  
Black Cottonwood ◽  
Wall Formation ◽  
Wood Development ◽  
Genome Wide ◽  
Secondary Wall Formation

Abstract Background: Malectin domain (MD) is a ligand-binding protein motif of pro- and eukaryotes. It is particularly abundant in Viridiplantae, where it occurs as either a single (MD, PF1721) or tandemly duplicated domain (PF12819) called malectin-like domain (MLD). In herbaceous plants, MD- or MLD-containing proteins (MD proteins) are known to regulate development, reproduction, and resistance to various stresses. However, their functions in woody plants have not yet been studied. To unravel their potential role in wood development, we carried out genome-wide identification of MD proteins in the model tree species black cottonwood (Populus trichocarpa), and analyzed their in-silico expression and co-expression networks.Results: P. trichocarpa had 146 MD genes assigned to 14 different clades, two of which were specific to the genus Populus. 87% of these genes were located on chromosomes, the rest being associated with scaffolds. Based on their protein domain organization, and in agreement with the exon-intron structures, the MD genes identified could be classified into five superclades having the following domains: leucine-rich repeat (LRR)-MD-protein kinase (PK), MLD-LRR-PK, MLD-PK (CrRLK1L), MLD-LRR, and MD-Kinesin. Whereas the majority of MD genes were highly expressed in leaves, particularly under stress conditions, eighteen showed a peak of expression during secondary wall formation and their co-expression networks suggested signaling functions in cell wall integrity, pathogen-associated molecular patterns, calcium, ROS, and hormone pathways.Conclusion: P. trichocarpa MD genes exhibit a variety of domain organizations, and include genes apparently specific to Populus, as well as genes with potential involvement in signaling pathways regulating secondary wall formation.

scholarly journals Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood

2019 ◽  
Vol 40 (1) ◽  
pp. 73-89 ◽  
Author(s):  
Manzar Abbas ◽  
Ilona Peszlen ◽  
Rui Shi ◽  
Hoon Kim ◽  
Rui Katahira ◽  
...  
Keyword(s):  
Solid State ◽  
Mechanical Strength ◽  
Cell Walls ◽  
Secondary Wall ◽  
Populus Trichocarpa ◽  
Wood Formation ◽  
Fiber Cell ◽  
Modulus Of Rupture ◽  
Cellulose Content ◽  
Wall Formation

Abstract Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.

scholarly journals The Dual Functions of WLIM1a in Cell Elongation and Secondary Wall Formation in Developing Cotton Fibers

2013 ◽  
Vol 25 (11) ◽  
pp. 4421-4438 ◽  
Author(s):  
L.-B. Han ◽  
Y.-B. Li ◽  
H.-Y. Wang ◽  
X.-M. Wu ◽  
C.-L. Li ◽  
...  
Keyword(s):  
Cell Elongation ◽  
Secondary Wall ◽  
Cotton Fibers ◽  
Wall Formation ◽  
Secondary Wall Formation ◽  
Dual Functions

Walls are thin 1 (WAT1), an Arabidopsis homolog of Medicago truncatula NODULIN21, is a tonoplast-localized protein required for secondary wall formation in fibers

2010 ◽  
Vol 63 (3) ◽  
pp. 469-483 ◽  
Author(s):  
Philippe Ranocha ◽  
Nicolas Denancé ◽  
Ruben Vanholme ◽  
Amandine Freydier ◽  
Yves Martinez ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Secondary Wall ◽  
Wall Formation ◽  
Secondary Wall Formation ◽  
Arabidopsis Homolog

scholarly journals The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus

2012 ◽  
Vol 194 (1) ◽  
pp. 102-115 ◽  
Author(s):  
Eryang Li ◽  
Apurva Bhargava ◽  
Weiya Qiang ◽  
Michael C. Friedmann ◽  
Natascha Forneris ◽  
...  
Keyword(s):  
Secondary Wall ◽  
Class Ii ◽  
Knox Gene ◽  
Wall Formation ◽  
Secondary Wall Formation

scholarly journals A Transcriptional and Metabolic Framework for Secondary Wall Formation in Arabidopsis

2016 ◽  
pp. pp.01100.2016 ◽  
Author(s):  
Zheng Li ◽  
Nooshin Omranian ◽  
Lutz Neumetzler ◽  
ting wang ◽  
Thomas Herter ◽  
...  
Keyword(s):  
Secondary Wall ◽  
Wall Formation ◽  
Secondary Wall Formation
Sign in / Sign up

Export Citation Format

Share Document