scholarly journals Erratum: Corrigendum: 3D Structural Fluctuation of IgG1 Antibody Revealed by Individual Particle Electron Tomography

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xing Zhang ◽  
Lei Zhang ◽  
Huimin Tong ◽  
Bo Peng ◽  
Matthew J. Rames ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Individual Particle ◽  
Igg1 Antibody ◽  
Structural Fluctuation

scholarly journals 3D Structural Fluctuation of IgG1 Antibody Revealed by Individual Particle Electron Tomography

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Xing Zhang ◽  
Lei Zhang ◽  
Huimin Tong ◽  
Bo Peng ◽  
Matthew J. Rames ◽  
...  
Keyword(s):  
Protein Dynamics ◽  
Electron Tomography ◽  
3D Structure ◽  
Three Dimensional ◽  
Individual Particle ◽  
Single Particle Reconstruction ◽  
X Ray Crystallography ◽  
Igg1 Antibody ◽  
Structural Fluctuation ◽  
Dynamics Simulations

Abstract Commonly used methods for determining protein structure, including X-ray crystallography and single-particle reconstruction, often provide a single and unique three-dimensional (3D) structure. However, in these methods, the protein dynamics and flexibility/fluctuation remain mostly unknown. Here, we utilized advances in electron tomography (ET) to study the antibody flexibility and fluctuation through structural determination of individual antibody particles rather than averaging multiple antibody particles together. Through individual-particle electron tomography (IPET) 3D reconstruction from negatively-stained ET images, we obtained 120 ab-initio 3D density maps at an intermediate resolution (~1–3 nm) from 120 individual IgG1 antibody particles. Using these maps as a constraint, we derived 120 conformations of the antibody via structural flexible docking of the crystal structure to these maps by targeted molecular dynamics simulations. Statistical analysis of the various conformations disclosed the antibody 3D conformational flexibility through the distribution of its domain distances and orientations. This blueprint approach, if extended to other flexible proteins, may serve as a useful methodology towards understanding protein dynamics and functions.

scholarly journals Single-Molecule 3D Images of “Hole-Hole” IgG1 Homodimers by Individual-Particle Electron Tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dongsheng Lei ◽  
Jianfang Liu ◽  
Hongbin Liu ◽  
Thomas E. Cleveland ◽  
John P. Marino ◽  
...  
Keyword(s):  
Single Molecule ◽  
Electron Tomography ◽  
Individual Particle ◽  
3D Images

scholarly journals Structure of 9.6nm Discoidal High-Density Lipoprotein Revealed by Individual-Particle Electron Tomography

2010 ◽  
Vol 98 (3) ◽  
pp. 440a
Author(s):  
Lei Zhang ◽  
Giorgio Cavigiolio ◽  
Jianjun Wang ◽  
Kerry-Anne Rye ◽  
Michael Oda ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Electron Tomography ◽  
Density Lipoprotein ◽  
High Density ◽  
Individual Particle

scholarly journals Determination of the Dynamic Structures of Igg Antibody by Individual-Particle Electron Tomography

2014 ◽  
Vol 106 (2) ◽  
pp. 251a
Author(s):  
Xing Zhang ◽  
Lei Zhang ◽  
Matthew J. Rames ◽  
Gang Ren
Keyword(s):  
Electron Tomography ◽  
Individual Particle ◽  
Igg Antibody ◽  
Dynamic Structures
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