scholarly journals Identification and comparative analysis of complement C3-associated microRNAs in immune response of Apostichopus japonicus by high-throughput sequencing

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Lei Zhong ◽  
Feng Zhang ◽  
Yu Zhai ◽  
Yanhui Cao ◽  
Si Zhang ◽  
...  
Keyword(s):  
Immune Response ◽  
Comparative Analysis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Apostichopus Japonicus ◽  
Complement C3

Comparative analysis of paddy straw-degrading consortia in China using high-throughput sequencing

2021 ◽  
Vol 167 ◽  
pp. 104077
Author(s):  
Yunhe Ban ◽  
Xiang Li ◽  
Yuqi Li ◽  
Xinyu Li ◽  
Xu Li ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Paddy Straw

scholarly journals Investigation of the Plasma Virome from Cases of Unexplained Febrile Illness in Tanzania from 2013 to 2014: a Comparative Analysis between Unbiased and VirCapSeq-VERT High-Throughput Sequencing Approaches

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
Author(s):  
Simon H. Williams ◽  
Samuel Cordey ◽  
Nishit Bhuva ◽  
Florian Laubscher ◽  
Mary-Anne Hartley ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Positive Selection ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Viral Infections ◽  
Febrile Illness ◽  
Clinical Samples ◽  
Barr Virus ◽  
Outbreak Investigations ◽  
Epstein Barr

ABSTRACT High-throughput sequencing can provide insights into epidemiology and medicine through comprehensive surveys of viral genetic sequences in environmental and clinical samples. Here, we characterize the plasma virome of Tanzanian patients with unexplained febrile illness by using two high-throughput sequencing methods: unbiased sequencing and VirCapSeq-VERT (a positive selection system). Sequences from dengue virus 2, West Nile virus, human immunodeficiency virus type 1, human pegivirus, and Epstein-Barr virus were identified in plasma. Both sequencing strategies recovered nearly complete genomes in samples containing multiple viruses. Whereas VirCapSeq-VERT had better sensitivity, unbiased sequencing provided better coverage of genome termini. Together, these data demonstrate the utility of high-throughput sequencing strategies in outbreak investigations. IMPORTANCE Characterization of the viruses found in the blood of febrile patients provides information pertinent to public health and diagnostic medicine. PCR and culture have historically played an important role in clinical microbiology; however, these methods require a targeted approach and may lack the capacity to identify novel or mixed viral infections. High-throughput sequencing can overcome these constraints. As the cost of running multiple samples continues to decrease, the implementation of high-throughput sequencing for diagnostic purposes is becoming more feasible. Here we present a comparative analysis of findings from an investigation of unexplained febrile illness using two strategies: unbiased high-throughput sequencing and VirCapSeq-VERT, a positive selection high-throughput sequencing system.

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