scholarly journals Streptococcus agalactiae Serotype Distribution and Antimicrobial Susceptibility in Pregnant Women in Gabon, Central Africa

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Sabine Belard ◽  
Nicole Toepfner ◽  
Mesküre Capan-Melser ◽  
Ghyslain Mombo-Ngoma ◽  
Rella Zoleko-Manego ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Central Africa ◽  
Serotype Distribution

scholarly journals Antimicrobial susceptibility and serotype distribution of Streptococcus agalactiae recto-vaginal colonizing isolates from pregnant women at a tertiary hospital in Pretoria, South Africa: an observational descriptive study

2019 ◽  
Author(s):  
Mohamed Said ◽  
Yusuf Dangor ◽  
Nontombi Mbelle ◽  
Shabir A. Madhi ◽  
Gaurav Kwatra ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Invasive Disease ◽  
Test Method ◽  
Serotype Distribution ◽  
Intrapartum Antibiotic Prophylaxis ◽  
Intermediate Resistance ◽  
Intrapartum Antibiotic

AbstractIntroductionStreptococcus agalactiae or Group B Streptococcus (GBS) is a significant cause of neonatal sepsis. Intrapartum antibiotic prophylaxis is recommended for pregnant women identified to be recto-vaginally colonised between 34-37 weeks gestational age to decrease the risk of invasive disease in their newborns. The aim of this study was to investigate serotype distribution and antimicrobial susceptibility patterns of GBS isolates cultured from recto-vaginal specimens during pregnancy.MethodsSixty-nine archived maternal colonizing isolates were tested against penicillin, erythromycin, clindamycin, vancomycin and levofloxacin. Minimum Inhibitory Concentration (MIC) testing was performed using the E-test method. Serotyping was performed by latex agglutination method.ResultsThe most common serotypes detected were Ia (54%), III (20%), V (16%), II (6%), IV (2%) and Ib (1%), respectively. All isolates were fully susceptible to penicillin, vancomycin and levofloxacin. Eight (11%) and 50 (56%) isolates showed intermediate resistance to erythromycin and clindamycin respectively, and one isolate was resistant to erythromycin. MLSB phenomenon was noted in 3 (4%) of the isolates.ConclusionGBS colonizing isolates remain susceptible to penicillin and remains the drug of choice for intrapartum antibiotic prophylaxis and treatment of invasive disease in newbrons. Macrolides should only be used if clinically indicated due to the high prevalence of intermediate resistance. A hexavalent GBS vaccine currently under development would provide coverage for 100% of the isolates identified in this study.

scholarly journals Streptococcus agalactiae in pregnant women: serotype and antimicrobial susceptibility patterns over five years in Eastern Sicily (Italy)

2020 ◽  
Vol 39 (12) ◽  
pp. 2387-2396
Author(s):  
Carlo Genovese ◽  
Floriana D’Angeli ◽  
Valentina Di Salvatore ◽  
Gianna Tempera ◽  
Daria Nicolosi
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Late Onset ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Disk Diffusion Method ◽  
Defibrinated Sheep Blood ◽  
Disk Diffusion Test

AbstractStreptococcus agalactiae (also known Group B Streptococcus or GBS) represents the main pathogen responsible for early- and late-onset infections in newborns. The present study aimed to determine the antimicrobial susceptibility pattern and the capsular serotypes of GBS isolated in Eastern Sicily over 5 years, from January 2015 to December 2019. A total of 3494 GBS were isolated from vaginal swabs of pregnant women (37–39 weeks), as recommended by the Centers for Disease Control and Prevention. Capsular polysaccharide’s typing of GBS was determined by a commercial latex agglutination test containing reagents to serotypes I–IX. The antimicrobial resistance pattern of GBS was determined through the disk diffusion method (Kirby-Bauer) and the double-disk diffusion test on Mueller-Hinton agar plates supplemented with 5% defibrinated sheep blood, according to the guidelines of the Clinical and Laboratory Standards Institute. Serotypes III (1218, 34.9%) and V (1069, 30.6%) were the prevalent colonizers, followed by not typable (570, 16.3%) and serotypes Ia (548, 15.7%), Ib (47, 1.3%), II (40, 1.1%), and IV (2, 0.1%). All 3494 clinical isolates were susceptible to cefditoren and vancomycin. Resistance to penicillin, ampicillin, levofloxacin, clindamycin, and erythromycin was observed in 6 (0.2%), 5 (0.1%), 161 (4.6%), 1090 (31.2%), and 1402 (40.1%) of the strains, respectively. Most of erythromycin-resistant GBS (1090/1402) showed the cMLSB phenotype, 276 the M phenotype, and 36 the iMLSB phenotype. Our findings revealed a higher prevalence of serotype III and a relevant resistance rate, among GBS strains, to the most frequently used antibiotics in antenatal screening.

scholarly journals Streptococcus agalactiae: Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women

2020 ◽  
Vol 8 (18) ◽  
pp. 3988-3998
Author(s):  
Fabrícia Almeida Fernandes Santana ◽  
Tais Viana Ledo de Oliveira ◽  
Marcelo Barreto de Souza Filho ◽  
Lucas Santana Coelho da Silva ◽  
Breno Bittencourt de Brito ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Resistance Genes ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Identification Methods ◽  
Susceptibility And Resistance

scholarly journals Prevalence and antimicrobial susceptibility profile of Streptococcus agalactiae in pregnant women seen at the University Hospital of Londrina, Paraná, Brazil

2018 ◽  
Vol 39 (1) ◽  
pp. 77
Author(s):  
Ana Elisa Belotto Morguette ◽  
Renata Perugini Biasi-Garbin ◽  
Eliane Saori Otaguiri ◽  
Marcia Regina Eches Perugini ◽  
Marsileni Pelisson ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
University Hospital ◽  
Antimicrobial Susceptibility Profile ◽  
Para Determinar ◽  
The University

Um estudo retrospectivo foi realizado com gestantes atendidas no Hospital Universitário de Londrina, Paraná, Brasil para determinar a prevalência de colonização vaginal-retal por Estreptococos do Grupo B (EGB) e o perfil de sensibilidade de EGB aos antimicrobianos utilizados para a antibioticoterapia profilática intraparto. Swabs vaginais-retais foram coletados de 2.901 mulheres entre a 35ª e 37ª semana de gestação. Destes, 528 (18,2%) apresentaram cultura positiva para EGB, e 0,4%, 10,2% e 10% dos isolados foram resistentes à penicilina, eritromicina e clindamicina, respectivamente. Estes resultados destacam a importância de vigilância contínua da colonização por EGB em gestantes para a prevenção de infecções em neonatos por EGB.

scholarly journals Prevalence of Streptococcus agalactiae Serotypes Associated with Anogenital Colonization among Pregnant Women in Jos

2019 ◽  
pp. 1-7
Author(s):  
Dahal A. Samuel ◽  
Daniel Z. Egah ◽  
Kandakai-Olukemi T. Yvonne
Keyword(s):  
Health Care ◽  
Pregnant Women ◽  
Teaching Hospital ◽  
Streptococcus Agalactiae ◽  
Prevalence Rate ◽  
University Teaching ◽  
Colonization Rate ◽  
Systematic Sampling ◽  
Serotype Distribution ◽  
Study Population

Aim: The aim of this study was to determine the prevalence of GBS serotype distribution in Jos University Teaching Hospital. Materials and Methodology: This was a hospital based descriptive cross-sectional study of 300 women receiving health care at the Jos University Teaching Hospital between July 2017 and November 2017. Systematic sampling technique was employed in recruiting consenting subjects for this study. High vaginal and anorectal swabs were collected from each subject after obtaining their consent by signing a structured consent form. The identified Streptococcus agalactiae (GBS) isolates were serotyped using immuLex strep-B antisera from SSI Diagnostica, 2 Herredysvejen, DK-3400 Hillerod Demnark to identify the different serotypes. The results obtained were computed using SPSS version 21. Results: A total of 300 women obtaining health care in Jos University Teaching Hospital (JUTH) were enrolled in this study between the months of July, 2017 and November, 2017. In all, vaginal and anorectal swabs were taken from 200 pregnant women and 100 non-pregnant women. The age range of the study population was between 16 years to 48 years with a mean age of 31.9 year (SD ± 6.6). The prevalence rate among the study population was 6.3%. The colonization rate among pregnant and non-pregnant women was 6.5% and 6.0% respectively with no significant statistical difference. Serotype Ia was the commonest isolate responsible for 42.1% of the GBS isolates. Serotype III accounted for 31.6% of the isolates, followed by serotype V (15.8%). Serotype II was less common, responsible for only 10.5%. Conclusion: This study showed that GBS colonization rate among the study population was 6.3%. Approximately, 6.5% and 6.0% prevalence rate were found among pregnant and non-pregnant women respectively. Of all the GBS isolates, serotypes Ia, II, III, and serotype V were isolated with serotype Ia being the most prevalent serotype. This knowledge of serotype distribution will help in instituting serotype specific GBS vaccines for the prevention of GBS diseases in Jos.

Antimicrobial susceptibility and genetic diversity of Streptococcus agalactiae recovered from newborns and pregnant women in Brazil

2013 ◽  
Vol 45 (10) ◽  
pp. 780-785 ◽  
Author(s):  
Viviane C. Souza ◽  
Fabíola C. O. Kegele ◽  
Selma R. Souza ◽  
Felipe P. G. Neves ◽  
Geraldo R. de Paula ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae

Suscetibilidade antimicrobiana de Streptococcus agalactiae isolados de gestantes em um hospital materno infantil de Porto Alegre, Rio Grande do Sul

2018 ◽  
Vol 28 (3) ◽  
pp. 30246
Author(s):  
Fernanda Rieth Battistin ◽  
Mariana Preussler Mott ◽  
Cícero Armídio Gomes Dias ◽  
Vinicius Pietta Perez
Keyword(s):  
Antibiotic Prophylaxis ◽  
Pregnant Women ◽  
Antimicrobial Susceptibility ◽  
Streptococcus Agalactiae ◽  
Penicillin G ◽  
Beta Lactam ◽  
Porto Alegre ◽  
Intrapartum Antibiotic Prophylaxis ◽  
Intrapartum Antibiotic ◽  
Child Hospital

AIMS: To characterize the antimicrobial susceptibility profile of Streptococcus agalactiae isolated from pregnant women attended at a public hospital.METHODS: The study was carried out in a public maternal and child hospital in Porto Alegre, RS, Brazil, in which the screening for S. agalactiae in pregnant women is part of the obstetrics routine. The study was carried out on anal/vaginal swab tests performed from July 2015 to February 2016. Bacterial isolates were identified by phenotypic tests, and the susceptibility to ampicillin, clindamycin, erythromycin and ofloxacin was determined. The erythromycin resistance genes ermB and mefA were also investigated.RESULTS: A total of 294 samples were included, and of these, 26 (8%) were positive for S. agalactiae. All isolates were susceptible to ampicillin, and resistance to erythromycin (21.4%), clindamycin (14.3%) and ofloxacin (7.1%) were observed. The mefA genotype was observed in 66% of the erythromycin resistant isolates.CONCLUSIONS: Results of this study corroborate the consensus that in pregnant women colonized with S. agalactiae, intrapartum antibiotic prophylaxis with penicillin G or ampicillin is indicated. The relevant proportion of isolates resistant to erythromycin and clindamycin, indicated for intrapartum antibiotic prophylaxis in case of allergy to beta-lactam antibiotics, emphasizes the importance of determining the profile of antimicrobial susceptibility of these isolates, a measure that is not yet part of routine prenatal tests in many institutions.

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