scholarly journals Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Chenchang Bao ◽  
Yanan Yang ◽  
Huiyang Huang ◽  
Haihui Ye
Keyword(s):  
Expression Profiles ◽  
Transcriptomic Analysis ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Cerebral Ganglia

scholarly journals mRNA profile provides novel insights into stress adaptation in mud crab megalopa, Scylla paramamosain after salinity stress

2020 ◽  
Author(s):  
Yin Zhang ◽  
Qingyang Wu ◽  
Shaobin Fang ◽  
Shengkang Li ◽  
Huaiping Zheng ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Salinity Stress ◽  
Expression Profiles ◽  
Scylla Paramamosain ◽  
Stress Adaptation ◽  
Comparative Genomic ◽  
Mud Crab ◽  
Short Term ◽  
Atpase Subunit ◽  
Mrna Profile

Abstract Background Mud crab, Scylla paramamosain, a euryhaline crustacean species, mainly inhabits the Indo-Western Pacific region. Wild mud crab spawn in high-salt condition and the salinity reduced with the growth of the hatching larvae. When the larvae grow up to megalopa, they migrate back to estuaries and coasts in virtue of the flood tide, settle and recruit adult habitats and metamorphose into the crablet stage. Adult crab can even survive in a wide salinity of 0–35 ppt. To investigate the mRNA profile after salinity stress, S. paramamosain megalopa were exposed to different salinity seawater (low, 14 ppt; control, 25 ppt; high, 39 ppt). Results Firstly, from the expression profiles of Na+/K+/2Cl- cotransporter, chloride channel protein 2 and ABC transporter. It turned out that the 24 h might be the most influenced duration in the short-term stress. We collected megalopa under different salinity for 24 h and then submitted to mRNA profiling. Totally, 57.87 Gb Clean Data were obtained. The comparative genomic analysis detected 342 differentially expressed genes (DEGs). According to the most significantly DEGs such as gamma-butyrobetaine dioxygenase-like, facilitated trehalose transporter Tret1, sodium/potassium-transporting ATPase subunit alpha, rhodanese 1-like protein, etc. and the significantly enriched pathways including lysine degradation, choline metabolism in cancer, phospholipase D signaling pathway, Fc gamma R-mediated phagocytosis, and sphingolipid signaling pathway. it indicated that in the short-term salinity stress, the megalopa might regulate some mechanism such as metabolism, immunity responses, osmoregulation to adapt to the alteration of the environment. Conclusions It indicated that the megalopa as a crab larvae has the ability to protect itself from the short-term salinity stress so as to live a migration life. This study mines beneficial genetic resources for research on crustacean and provides novel insight into understanding the molecular mechanism of stress adaptation of crustacean responses to environmental stressors.

Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

2013 ◽  
Vol 35 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Wen-Xing Li ◽  
Hui-Yang Huang ◽  
Jing-Ru Huang ◽  
Jin-Jin Yu ◽  
Jun Ma ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Molecular Cloning ◽  
Expression Profiles ◽  
Cyclin B ◽  
Scylla Paramamosain ◽  
Mud Crab

Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain

2012 ◽  
Vol 32 (4) ◽  
pp. 503-512 ◽  
Author(s):  
Shengkang Li ◽  
Zhao Zhang ◽  
Chuanbiao Li ◽  
Lizhen Zhou ◽  
Wenhua Liu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Molecular Cloning ◽  
Expression Profiles ◽  
Cloning And Expression ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Oxide Synthase

scholarly journals dLp/HDL-BGBP and MTP Cloning and Expression Profiles During Embryonic Development in the Mud Crab Scylla paramamosain

2021 ◽  
Vol 12 ◽  
Author(s):  
Xianyuan Zeng ◽  
Liang Lyu ◽  
Dousha Zhao ◽  
Jinying Zhong ◽  
Yan Feng ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Expression Profiles ◽  
Cloning And Expression ◽  
Scaffolding Protein ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Pigment Formation ◽  
Transfer Protein ◽  
Formation Stage ◽  
Appendage Formation

Lipids are the main energy source for embryonic development in oviparous animals. Prior to the utilization and catabolism, lipids are primarily transported from the yolk sac to embryonic tissues. In the present study, cDNA encoding a circulatory large lipid transfer protein (LLTP) superfamily member, the precursor of large discoidal lipoprotein (dLp) and high-density lipoprotein/β-1,3-glucan-binding protein (HDL-BGBP), named dLp/HDL-BGBP of 14,787 bp in length, was cloned from the mud crab Scylla paramamosain. dLp/HDL-BGBP was predicted to encode a 4,831 amino acids (aa) protein that was the precursor of dLp and HDL-BGBP, which were both detected in hemolymph by liquid chromatography–mass spectrometry (LC-MS/MS) analysis. For the intracellular LLTP, three microsomal triglyceride transfer protein (MTP) cDNAs of 2,905, 2,897, and 3,088 bp in length were cloned from the mud crab and were predicted to encode MTP-A of 881 aa, MTP-B of 889 aa, and MTP-C of 919 aa, respectively, which were different merely in the N-terminal region and shared an identical sequence of 866 aa. During embryonic development, the expression level of dLp/HDL-BGBP consecutively increased from the early appendage formation stage to the eye pigment-formation stage, which indicated that HDL-BGBP is probably the scaffolding protein for yolk lipid. For the MTP gene, MTP-C accounted for ~70% of MTP mRNA from the blastocyst stage to the nauplius stage, as well as the pre-hatching stage; MTP-C and MTP-A expression levels were comparable from the early appendage formation stage to the late eye pigment-formation stage; MTP-A was extremely low in blastocyst and gastrula stages; MTP-B was expressed at a relatively low-level throughout embryo development. The variations in the expression profiles among MTP transcripts suggested that MTP might play roles in the lipid droplet maturation and lipoprotein assembly during embryonic development.

The full-length cDNA cloning and expression profiles of 14-3-3 genes from the mud crab Scylla paramamosain Estampador, 1949

Crustaceana ◽  
2015 ◽  
Vol 88 (10-11) ◽  
pp. 1065-1078 ◽  
Author(s):  
Miao-An Shu ◽  
Chen Long ◽  
Wei-Ren Dong ◽  
Pan Zhang ◽  
Bin-Peng Xu ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Expression Patterns ◽  
Full Length ◽  
Cloning And Expression ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Phylogenetic Tree Analysis ◽  
Specific Expression ◽  
Full Length Cdna ◽  
Cdna Sequences

Two full-length cDNA sequences of 14-3-3 genes were isolated from the mud crab, Scylla paramamosain Estampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both 14-3-3 genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp14-3-3 genes show 96 and 97% identity, respectively, and both Sp14-3-3 cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp14-3-3 genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp14-3-3 genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp14-3-3 genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the 14-3-3 family in both crustaceans and (in)vertebrates as a whole.

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