scholarly journals Novel High Content Screen Detects Compounds That Promote Neurite Regeneration from Cochlear Spiral Ganglion Neurons

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Donna S. Whitlon ◽  
Mary Grover ◽  
Sara F. Dunne ◽  
Sonja Richter ◽  
Chi-Hao Luan ◽  
...  
Keyword(s):  
Spiral Ganglion ◽  
Spiral Ganglion Neurons ◽  
Cochlear Spiral Ganglion ◽  
Ganglion Neurons

The Cochlear Spiral Ganglion Neurons: The Auditory Portion of the VIII Nerve

2018 ◽  
Vol 302 (3) ◽  
pp. 463-471 ◽  
Author(s):  
Francisco Carricondo ◽  
Bárbara Romero-Gómez
Keyword(s):  
Spiral Ganglion ◽  
Spiral Ganglion Neurons ◽  
Cochlear Spiral Ganglion ◽  
Ganglion Neurons

scholarly journals Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Chao Li ◽  
Xiang Li ◽  
Zhenghong Bi ◽  
Ken Sugino ◽  
Guangqin Wang ◽  
...  
Keyword(s):  
Spiral Ganglion ◽  
Cell Types ◽  
Research Field ◽  
Mouse Strains ◽  
Spiral Ganglion Neurons ◽  
Rna Seq ◽  
Gene Coverage ◽  
Cochlear Spiral Ganglion ◽  
Transcriptome Comparison ◽  
Ganglion Neurons

Inner ear cochlear spiral ganglion neurons (SGNs) transmit sound information to the brainstem. Recent single cell RNA-Seq studies have revealed heterogeneities within SGNs. Nonetheless, much remains unknown about the transcriptome of SGNs, especially which genes are specifically expressed in SGNs. To address these questions, we needed a deeper and broader gene coverage than that in previous studies. We performed bulk RNA-Seq on mouse SGNs at five ages, and on two reference cell types (hair cells and glia). Their transcriptome comparison identified genes previously unknown to be specifically expressed in SGNs. To validate our dataset and provide useful genetic tools for this research field, we generated two knockin mouse strains: Scrt2-P2A-tdTomato and Celf4-3xHA-P2A-iCreER-T2A-EGFP. Our comprehensive analysis confirmed the SGN-selective expression of the candidate genes, testifying to the quality of our transcriptome data. These two mouse strains can be used to temporally label SGNs or to sort them.

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