scholarly journals Erratum: Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Weicong Qi ◽  
Iris E. M. Tinnenbroek-Capel ◽  
Elma M. J. Salentijn ◽  
Jan G. Schaart ◽  
Jihua Cheng ◽  
...  
Keyword(s):  
Crambe Abyssinica ◽  
Marker Free ◽  
Chemical Selection

scholarly journals Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Weicong Qi ◽  
Iris E. M. Tinnenbroek-Capel ◽  
Elma M. J. Salentijn ◽  
Jan G. Schaart ◽  
Jihua Cheng ◽  
...  
Keyword(s):  
Gene Fusion ◽  
Negative Selection ◽  
Free System ◽  
Crambe Abyssinica ◽  
Stepwise Selection ◽  
Recombinant Cells ◽  
Extremely High Frequency ◽  
Marker Free ◽  
Dna Fragment ◽  
Chemical Selection

Abstract The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying dexamethasone (DEX) can activate the recombinase to remove the T-DNA fragment between recombination sites. The recombinant ought to be selected on 5-fluorocytocine (5-FC) because of codA converting 5-FC into 5-fluorouracil the toxic. A PMF1 vector was transformed into hexaploid species Crambe abyssinica. Two independent transformants were chosen for DEX-induced recombination and later 5-FC selection. In contrast to earlier pMF1 experiments, the strategy of stepwise selection based on meristematic regeneration was engaged. After a long period of 5-FC selection, recombinants were obtained successfully, but most of the survivors were wildtype and non-recombinant. The results revealed when applying the PMF1 marker-free system on C. abyssinica, 1) Increasing in the DEX concentration did not correspondingly enhance the success of recombination; 2) both of the DEX-induced recombination and 5-FC negative selection were apparently insufficient which was leading to the extremely high frequency in chimerism occurring for recombinant and non-recombinant cells in tissues; 3) the strategy of stepwise selection based on meristem tissue regeneration was crucial for successfully isolating the recombinant germplasm from the chimera.

LIGHT INFLUENCE ON SEED GERMINATION OF CRAMBE (Crambe abyssinica Hochst.)

Nucleus ◽  
2011 ◽  
Vol 8 (1) ◽  
pp. 405-412 ◽  
Author(s):  
Lima Deleon Martins ◽  
Felipe Pianna Costa ◽  
José Carlos Lopes
Keyword(s):  
Seed Germination ◽  
Crambe Abyssinica

Production of γ-Linolenic Acid and Stearidonic Acid in Seeds of Marker-Free Transgenic Soybean

Crop Science ◽  
2004 ◽  
Vol 44 (2) ◽  
pp. 646 ◽  
Author(s):  
Shirley Sato ◽  
Aiqiu Xing ◽  
Xingguo Ye ◽  
Bruce Schweiger ◽  
Anthony Kinney ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Transgenic Soybean ◽  
Marker Free

scholarly journals Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation

2017 ◽  
Vol 5 (4) ◽  
pp. 271-281 ◽  
Author(s):  
Dan Feng ◽  
Yanwei Wang ◽  
Jinxia Wu ◽  
Tiegang Lu ◽  
Zhiguo Zhang
Keyword(s):  
Drought Tolerance ◽  
Transgenic Rice ◽  
Marker Free

Deep learning-based approach using X-ray images for classifying Crambe abyssinica seed quality

2021 ◽  
Vol 164 ◽  
pp. 113378 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Rodrigo Cupertino Bernardes ◽  
Laércio Junio da Silva ◽  
Bruno Antônio Lemos de Freitas ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
...  
Keyword(s):  
Deep Learning ◽  
Seed Quality ◽  
Crambe Abyssinica ◽  
X Ray

Rapid visualized assessment of drug efficacy in live mice with a selectable marker-free autoluminescent Klebsiella pneumoniae

2021 ◽  
Vol 177 ◽  
pp. 112919
Author(s):  
Xirong Tian ◽  
Yamin Gao ◽  
Shuai Wang ◽  
H.M. Adnan Hameed ◽  
Wei Yu ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Selectable Marker ◽  
Drug Efficacy ◽  
Marker Free

A marker-free alignment method for electron tomography

1995 ◽  
Vol 58 (3-4) ◽  
pp. 393-402 ◽  
Author(s):  
Yu Liu ◽  
Pawel A Penczek ◽  
Bruce F McEwen ◽  
Joachim Frank
Keyword(s):  
Electron Tomography ◽  
Alignment Method ◽  
Marker Free

scholarly journals Targeted Allele Replacement Mutagenesis of Corynebacterium pseudotuberculosis

2011 ◽  
Vol 77 (10) ◽  
pp. 3532-3535 ◽  
Author(s):  
Caray A. Walker ◽  
Willie Donachie ◽  
David G. E. Smith ◽  
Michael C. Fontaine
Keyword(s):  
Corynebacterium Pseudotuberculosis ◽  
Sequence Length ◽  
Homologous Sequence ◽  
Proof Of Concept ◽  
Rolling Circle Replication ◽  
Content Type ◽  
Rolling Circle ◽  
Allele Replacement ◽  
Marker Free ◽  
The Relationship

ABSTRACTA two-step allele replacement mutagenesis procedure, using a conditionally replicating plasmid, was developed to allow the creation of targeted, marker-free mutations inCorynebacterium pseudotuberculosis. The relationship between homologous sequence length and recombination frequency was determined, and enhanced plasmid excision was observed due to the rolling-circle replication of the mutagenesis vector. Furthermore, an antibiotic enrichment procedure was applied to improve the recovery of mutants. Subsequently, as proof of concept, a marker-free,cp40-deficient mutant ofC. pseudotuberculosiswas constructed.

scholarly journals Dance motion capture and composition using multiple RGB and depth sensors

2017 ◽  
Vol 13 (2) ◽  
pp. 155014771769608 ◽  
Author(s):  
Yejin Kim
Keyword(s):  
Motion Capture ◽  
High Speed ◽  
Motion Path ◽  
High Complexity ◽  
Motion Data ◽  
Depth Sensors ◽  
Motion Editing ◽  
Human Movements ◽  
Marker Free ◽  
Motion Search

Dynamic human movements such as dance are difficult to capture without using external markers due to the high complexity of a dancer’s body. This article introduces a marker-free motion capture and composition system for dance motion that uses multiple RGB and depth sensors. Our motion capture system utilizes a set of high-speed RGB and depth sensors to generate skeletal motion data from an expert dancer. During the motion acquisition process, a skeleton tracking method based on a particle filter is provided to estimate the motion parameters for each frame from a sequence of color images and depth features retrieved from the sensors. The expert motion data become archived in a database. The authoring methods in our composition system automate most of the motion editing processes for general users by providing an online motion search with an input posture and then performing motion synthesis on an arbitrary motion path. Using the proposed system, we demonstrate that various dance performances can be composed in an intuitive and efficient way on client devices such as tablets and kiosk PCs.

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