scholarly journals Comparative proteomics reveals human pluripotent stem cell-derived limbal epithelial stem cells are similar to native ocular surface epithelial cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Alexandra Mikhailova ◽  
Antti Jylhä ◽  
Jochen Rieck ◽  
Janika Nättinen ◽  
Tanja Ilmarinen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Ocular Surface ◽  
Pluripotent Stem Cell ◽  
Comparative Proteomics ◽  
Human Pluripotent Stem Cell ◽  
Epithelial Stem Cells ◽  
Limbal Epithelial Stem Cells

Human pluripotent stem cell-derived limbal epithelial stem cells on bioengineered matrices for corneal reconstruction

2016 ◽  
Vol 146 ◽  
pp. 26-34 ◽  
Author(s):  
Alexandra Mikhailova ◽  
Tanja Ilmarinen ◽  
Anjula Ratnayake ◽  
Goran Petrovski ◽  
Hannu Uusitalo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Epithelial Stem Cells ◽  
Limbal Epithelial Stem Cells

scholarly journals Comparative Analysis of KnockOut™Serum with Fetal Bovine Serum for the In Vitro Long-Term Culture of Human Limbal Epithelial Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Shaokun Zhang ◽  
Zaoxia Liu ◽  
Guanfang Su ◽  
Hong Wu
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Epithelial Stem Cells ◽  
Fetal Bovine ◽  
Limbal Epithelial Stem Cells

The limbal epithelial cells can be maintained on 3T3 feeder layer with fetal bovine serum supplemented culture medium, and these cells have been used to successfully treat limbal stem cell deficiency. However, fetal bovine serum contains unknown components and displays quantitative and qualitative lot-to-lot variations. To improve the culture condition, the defined KnockOut serum replacement was investigated to replace fetal bovine serum for culturing human limbal epithelial cell. Human primary limbal epithelial cells were cultured in KnockOut serum and fetal bovine serum supplemented medium, respectively. The cell growth rate, gene expression, and maintenance of limbal epithelial stem cells were studied and compared between these two groups. Human primary limbal epithelial cells were isolated and successfully serially cultivated in this novel KnockOut serum supplemented medium; the cell proliferation and stem cell maintenance were similar to those of cells grown in fetal bovine serum supplemented medium. These data suggests that this KnockOut serum supplemented medium is an efficient replacement to traditional fetal bovine serum supplemented medium for limbal epithelial cell culture, and this medium has great potential for long term maintenance of limbal epithelial cells, limbal epithelial stem cells transplantation, and tissue regeneration.

scholarly journals Stem Cells in the Path of Light, from Corneal to Retinal Reconstruction

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 873
Author(s):  
Ovidiu Samoila ◽  
Lacramioara Samoila
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Clinical Success ◽  
Corneal Stroma ◽  
Cell Transplant ◽  
Inclusion Criteria ◽  
Epithelial Stem Cells ◽  
Corneal Epithelial ◽  
Stem Cells Transplantation ◽  
Limbal Epithelial Stem Cells

The future of eye reconstruction invariably includes stem cells transplantation. Corneal limbus, corneal stroma, trabeculum, retinal cells, optic nerve, and all structures that are irreversibly damaged and have no means to be repaired or replaced, through conventional treatment or surgery, represent targets for stem cell reconstruction. This review tries to answer the question if there is any clinical validation for stem therapies, so far, starting from the cornea and, on the path of light, arriving to the retina. The investigation covers the last 10 years of publications. From 2385 published sources, we found 56 clinical studies matching inclusion criteria, 39 involving cornea, and 17 involving retina. So far, corneal epithelial reconstruction seems well validated clinically. Enough clinical data are collected to allow some form of standardization for the stem cell transplant procedures. Cultivated limbal epithelial stem cells (CLET), simple limbal epithelial transplant (SLET), and oral mucosa transplantation are implemented worldwide. In comparison, far less patients are investigated in retinal stem reconstructions, with lower anatomical and clinical success, so far. Intravitreal, subretinal, and suprachoroidal approach for retinal stem therapies face specific challenges.

Substrates for Human Pluripotent Stem Cell Cultures in Conditioned Medium of Mesenchymal Stem Cells

2012 ◽  
Vol 23 (1-4) ◽  
pp. 153-165 ◽  
Author(s):  
Yusuke Ueda ◽  
Satoshi Fujita ◽  
Tatsuya Nishigaki ◽  
Yusuke Arima ◽  
Hiroo Iwata
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Cell Cultures ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell

scholarly journals Transplanted Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Support Liver Regeneration in Gunn Rats

2018 ◽  
Vol 27 (24) ◽  
pp. 1702-1714 ◽  
Author(s):  
Lucas-Sebastian Spitzhorn ◽  
Claus Kordes ◽  
Matthias Megges ◽  
Iris Sawitza ◽  
Silke Götze ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Liver Regeneration ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Gunn Rats

Histological analysis of epithelial stem cells during induced pluripotent stem cell-derived teratoma development

2012 ◽  
Vol 54 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Ryota Kishigami ◽  
Keishi Otsu ◽  
Ai Oikawa-Sasaki ◽  
Naoki Fujiwara ◽  
Kiyoto Ishizeki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Histological Analysis ◽  
Induced Pluripotent Stem Cell ◽  
Epithelial Stem Cells ◽  
Induced Pluripotent

scholarly journals Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Allergic Airway Inflammation in Mice

Stem Cells ◽  
2012 ◽  
Vol 30 (12) ◽  
pp. 2692-2699 ◽  
Author(s):  
Yue-Qi Sun ◽  
Meng-Xia Deng ◽  
Jia He ◽  
Qing-Xiang Zeng ◽  
Weiping Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Airway Inflammation ◽  
Pluripotent Stem Cell ◽  
Allergic Airway Inflammation ◽  
Human Pluripotent Stem Cell

scholarly journals Characterization of putative stem cells in isolated human colonic crypt epithelial cells and their interactions with myofibroblasts

2009 ◽  
Vol 296 (2) ◽  
pp. C296-C305 ◽  
Author(s):  
S. Samuel ◽  
R. Walsh ◽  
J. Webb ◽  
A. Robins ◽  
C. Potten ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Side Population ◽  
Cell Types ◽  
Population Characteristics ◽  
Colonic Crypt ◽  
Epithelial Stem Cells ◽  
Colonic Crypts ◽  
Cell Niche

Colonic epithelial stem cells are believed to be located at the crypt base where they have previously been shown to express musashi-1. The colonic stem cell niche, which includes extracellular matrix and myofibroblasts (together with other cell types), is likely to be important in maintaining the function of the progenitor cells. The aims of our studies were to characterize stem cells in isolated and disaggregated human colonic crypt epithelial cells and investigate their interactions with monolayers of primary human colonic myofibroblasts. In unfractionated preparations of disaggregated colonic crypts, musashi-1 positive cells preferentially adhered to colonic myofibroblasts, despite the presence of excess blocking anti-β1-integrin antibody. These adherent epithelial cells remained viable for a number of days and developed slender processes. Cells with side population characteristics (as demonstrated by ability to expel the dye Hoechst 33342) were consistently seen in the isolated colonic crypt epithelial cells. These side population cells expressed musashi-1, β1-integrin, BerEP4, and CD133. Sorted side population crypt epithelial cells also rapidly adhered to primary colonic myofibroblasts. In conclusion, in preparation of isolated and disaggregated human colonic crypts, cells with stem cell characteristics preferentially adhere to primary human colonic myofibroblasts in a β1-integrin-independent fashion.

scholarly journals Limbal stem cell deficiency: etiology, pathogenesis, priniciples and prospects of surgical treatment

2019 ◽  
Vol 12 (1) ◽  
pp. 103-111
Author(s):  
A. S. Dubovikov ◽  
I. O. Gavrilyuk ◽  
A. N. Kulikov ◽  
S. V. Churashov ◽  
V. F. Chernysh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Surgical Treatment ◽  
Ex Vivo ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cells ◽  
History Of Development ◽  
History Of ◽  
Limbal Epithelial Stem Cells ◽  
Limbal Stem Cell

The review is focused on the modern view of the etiology and pathogenesis of limbal stem cells deficiency. The history of development of tissue and ex-vivo transplantation of limbal epithelial stem cells is presented. Certain promising directions of the treatment of patients with limbal stem cells deficiency are presented.

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