scholarly journals Erratum: Corrigendum: Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ho Won Lee ◽  
Seung Yun Yoon ◽  
Thoudam Debraj Singh ◽  
Yoon Ju Choi ◽  
Hong Je Lee ◽  
...  
Keyword(s):  
Cell Migration ◽  
Dendritic Cell ◽  
Molecular Imaging ◽  
Lymph Nodes ◽  
Sodium Iodide ◽  
Firefly Luciferase ◽  
Sodium Iodide Symporter ◽  
Dendritic Cell Migration

scholarly journals Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ho Won Lee ◽  
Seung Yun Yoon ◽  
Thoudam Debraj Singh ◽  
Yoon Ju Choi ◽  
Hong Je Lee ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Molecular Imaging ◽  
Lymph Nodes ◽  
Injection Site ◽  
Reporter Gene ◽  
Sodium Iodide ◽  
Firefly Luciferase ◽  
Sodium Iodide Symporter ◽  
Reporter Gene Imaging ◽  
Pet Ct

Abstract We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

scholarly journals IRF4 Promotes Cutaneous Dendritic Cell Migration to Lymph Nodes during Homeostasis and Inflammation

2012 ◽  
Vol 189 (7) ◽  
pp. 3368-3377 ◽  
Author(s):  
Sandra Bajaña ◽  
Kimberly Roach ◽  
Sean Turner ◽  
Jinny Paul ◽  
Susan Kovats
Keyword(s):  
Cell Migration ◽  
Dendritic Cell ◽  
Lymph Nodes ◽  
Dendritic Cell Migration

scholarly journals CCR8 regulates contact hypersensitivity by restricting cutaneous dendritic cell migration to the draining lymph nodes

2014 ◽  
Vol 27 (4) ◽  
pp. 169-181 ◽  
Author(s):  
Rikio Yabe ◽  
Kenji Shimizu ◽  
Soichiro Shimizu ◽  
Satoe Azechi ◽  
Byung-Il Choi ◽  
...  
Keyword(s):  
Cell Migration ◽  
Dendritic Cell ◽  
Lymph Nodes ◽  
Contact Hypersensitivity ◽  
Dendritic Cell Migration ◽  
Draining Lymph Nodes
Sign in / Sign up

Export Citation Format

Share Document