scholarly journals Self-sorting heterodimeric coiled coil peptides with defined and tuneable self-assembly properties

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Christopher Aronsson ◽  
Staffan Dånmark ◽  
Feng Zhou ◽  
Per Öberg ◽  
Karin Enander ◽  
...  
Keyword(s):  
Self Assembly ◽  
Coiled Coil

Self-assembly of Stimuli Responsive Coiled-coil Fibrous Hydrogels

Soft Matter ◽  
2021 ◽  
Author(s):  
Michael Meleties ◽  
Priya Katyal ◽  
Bonnie Lin ◽  
Dustin Britton ◽  
Jin Kim Montclare
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Self Assembly ◽  
Coiled Coil ◽  
Stimuli Responsive ◽  
Tunable Properties ◽  
Stimuli Sensitive ◽  
Biomedical Fields

Owing to their tunable properties, hydrogels comprised of stimuli sensitive polymers are one of the most appealing scaffolds with applications in tissue engineering, drug delivery and other biomedical fields. We...

scholarly journals Colloidal stability versus self-assembly of nanoparticles controlled by coiled-coil protein interactions

Soft Matter ◽  
2019 ◽  
Vol 15 (36) ◽  
pp. 7122-7126
Author(s):  
Allison Siehr ◽  
Bin Xu ◽  
Ronald A. Siegel ◽  
Wei Shen
Keyword(s):  
Protein Interactions ◽  
Self Assembly ◽  
Colloidal Stability ◽  
Coiled Coil ◽  
Biomolecular Recognition

Orientational discrimination of biomolecular recognition is exploited to control nanoparticle self assembly and colloidal stability.

scholarly journals Rational Design of Helical Nanotubes from Self-assembly of Coiled-coil Lock Washers

2013 ◽  
Vol 19 (S2) ◽  
pp. 342-343
Author(s):  
C. Xu ◽  
E.R. Wright ◽  
A. Mehta ◽  
L.C. Ser-pell ◽  
X. Zuo ◽  
...  
Keyword(s):  
Self Assembly ◽  
Rational Design ◽  
Coiled Coil

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

Interfacial zippering-up of coiled-coil protein filaments

2015 ◽  
Vol 17 (46) ◽  
pp. 31055-31060 ◽  
Author(s):  
Emiliana De Santis ◽  
Valeria Castelletto ◽  
Maxim G. Ryadnov
Keyword(s):  
Self Assembly ◽  
De Novo ◽  
Coiled Coil ◽  
Morphological Control

A de novo self-assembly topology for engineering protein nanostructures under morphological control is reported.

scholarly journals Cross-linking reveals laminin coiled-coil architecture

2016 ◽  
Vol 113 (47) ◽  
pp. 13384-13389 ◽  
Author(s):  
Gad Armony ◽  
Etai Jacob ◽  
Toot Moran ◽  
Yishai Levin ◽  
Tevie Mehlman ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Self Assembly ◽  
Quaternary Structure ◽  
Coiled Coil ◽  
Surface Proteins ◽  
Cross Linking ◽  
Single Particle Reconstruction ◽  
Cross Links ◽  
Particle Reconstruction ◽  
Key Questions

Laminin, an ∼800-kDa heterotrimeric protein, is a major functional component of the extracellular matrix, contributing to tissue development and maintenance. The unique architecture of laminin is not currently amenable to determination at high resolution, as its flexible and narrow segments complicate both crystallization and single-particle reconstruction by electron microscopy. Therefore, we used cross-linking and MS, evaluated using computational methods, to address key questions regarding laminin quaternary structure. This approach was particularly well suited to the ∼750-Å coiled coil that mediates trimer assembly, and our results support revision of the subunit order typically presented in laminin schematics. Furthermore, information on the subunit register in the coiled coil and cross-links to downstream domains provide insights into the self-assembly required for interaction with other extracellular matrix and cell surface proteins.

Hierarchical Self-Assembly of a Coiled-Coil Peptide into Fractal Structure

Nano Letters ◽  
2005 ◽  
Vol 5 (7) ◽  
pp. 1255-1260 ◽  
Author(s):  
Andrea Lomander ◽  
Wonmuk Hwang ◽  
Shuguang Zhang
Keyword(s):  
Self Assembly ◽  
Fractal Structure ◽  
Coiled Coil

scholarly journals Complexity of Gas Vesicle Biogenesis in Halobacterium sp. Strain NRC-1: Identification of Five New Proteins

2004 ◽  
Vol 186 (10) ◽  
pp. 3182-3186 ◽  
Author(s):  
Hem Dutt Shukla ◽  
Shiladitya DasSarma
Keyword(s):  
Self Assembly ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Coiled Coil ◽  
Gas Vesicles ◽  
Immunoblotting Analysis ◽  
Large Gene ◽  
Vesicle Biogenesis ◽  
Gas Vesicle Proteins ◽  
Vesicle Proteins

ABSTRACT The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were previously detected. Here, we used immunoblotting analysis to probe for the presence of gas vesicle proteins corresponding to five additional gvp gene products. Polyclonal antisera were raised in rabbits against LacZ-GvpF, -GvpJ, and -GvpM fusion proteins and against synthetic 15-amino-acid peptides from GvpG and -L. Immunoblotting analysis was performed on cell lysates of wild-type Halobacterium sp. strain NRC-1, gas vesicle-deficient mutants, and purified gas vesicles, after purification of LacZ fusion antibodies on protein A and β-galactosidase affinity columns. Our results show the presence of five new gas vesicle proteins (GvpF, GvpG, GvpJ, GvpL, and GvpM), bringing the total number of proteins identified in the organelles to seven. Two of the new gas vesicle proteins are similar to GvpA (GvpJ and GvpM), and two proteins contain predicted coiled-coil domains (GvpF and GvpL). GvpL exhibited a multiplet ladder on sodium dodecyl sulfate-polyacrylamide gels indicative of oligomerization and self-assembly. We discuss the possible functions of the newly discovered gas vesicle proteins in biogenesis of these unique prokaryotic flotation organelles.

scholarly journals Nanostructure Formation from Three-Armed Coiled-Coil Peptide through Self-Assembly

2016 ◽  
Vol 73 (2) ◽  
pp. 175-182
Author(s):  
Shin-nosuke NISHIMURA ◽  
Tomoyuki KOGA ◽  
Nobuyuki HIGASHI
Keyword(s):  
Self Assembly ◽  
Coiled Coil ◽  
Nanostructure Formation
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