scholarly journals Functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Tong-Yi Dou ◽  
Hong-Wei Luan ◽  
Guang-Bo Ge ◽  
Ming-Ming Dong ◽  
Han-Fa Zou ◽  
...  
Keyword(s):  
Structural Properties ◽  
Multienzyme Complex ◽  
Glycoside Hydrolysis ◽  
Hydrolysis Of

STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE PRODUCED BY XANTHOMONAS ORYZAE

1962 ◽  
Vol 40 (12) ◽  
pp. 2204-2213 ◽  
Author(s):  
A. Misaki ◽  
S. Kirkwood ◽  
J. V. Scaletti ◽  
F. Smith
Keyword(s):  
Acid Hydrolysis ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Extracellular Polysaccharide ◽  
Xanthomonas Oryzae ◽  
Sodium Borohydride Reduction ◽  
Glycoside Hydrolysis ◽  
Hydrolysis Of ◽  
Structural Significance ◽  
Molecular Proportion

The extracellular polysaccharide isolated from cultures of Xanthomonas oryzae is composed of D-glucose (5 molecular proportions), D-glucuronic acid (2 molecular proportions), and D-mannose (5 molecular proportions). Acid hydrolysis of this polysaccharide, which contains 0.3% combined pyruvic acid, yields 2-O-β-D-glucopyranosyluronic acid D-mannose, which has been characterized as its crystalline fully methylated β-glycoside. Hydrolysis of the methylated polysaccharide gives 2,3,4,6-tetra-O-methyl-D-mannose (3 molecular proportions), 2,3,4-tri-O-methyl-D-glucuronic acid (1 molecular proportion), 2,3,6-tri-O-methyl-D-glucose (4 molecular proportions), 3,4,6-tri-O-methyl-D-mannose (2 molecular proportions), 2,6-di-O-methyl-D-glucose (3 molecular proportions), 2,3-di-O-methyl-D-glucose (1 molecular proportion). The polyalcohol derived from the polysaccharide by periodate oxidation followed by sodium borohydride reduction gives upon acid hydrolysis glycerol (2 molecular proportions), erythritol (1 molecular proportion), and D-glucose (1 molecular proportion). The general structural significance of these findings is discussed.

Effect of Alkali Pretreatment on the Structural Properties and Enzymatic Hydrolysis of Corn Cob

2012 ◽  
Vol 168 (7) ◽  
pp. 1806-1819 ◽  
Author(s):  
Padmavati Sahare ◽  
Rajkumar Singh ◽  
R. Seeta Laxman ◽  
Mala Rao
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Properties ◽  
Corn Cob ◽  
Alkali Pretreatment ◽  
Hydrolysis Of

scholarly journals Hydrolyses of α- and β-cellobiosyl fluorides by cellobiohydrolases of Trichoderma reesei

1993 ◽  
Vol 291 (3) ◽  
pp. 883-888 ◽  
Author(s):  
A K Konstantinidis ◽  
I Marsden ◽  
M L Sinnott
Keyword(s):  
Trichoderma Reesei ◽  
Biological Function ◽  
Fluoride Ion ◽  
Cellobiohydrolase I ◽  
Ion Release ◽  
Cellulase Complex ◽  
Cellobiohydrolase Ii ◽  
Glycoside Hydrolysis ◽  
Kinetics Of ◽  
Hydrolysis Of

Cellobiohydrolase II hydrolyses alpha- and beta-D-cellobiosyl fluorides to alpha-cellobiose at comparable rates, according to Michaelis-Menten kinetics. The stereochemistry, absence of transfer products and strict hyperbolic kinetics of the hydrolysis of alpha-cellobiosyl fluoride suggest that the mechanism for the alpha-fluoride may be the enzymic counterpart of the SNi reaction observed in the trifluoroethanolysis of alpha-glucopyranosyl fluoride [Sinnott and Jencks (1980) J. Am. Chem. Soc. 102, 2026-2032]. The absolute factors by which this enzyme accelerates fluoride ion release are small and greater for the alpha-fluoride than for the beta, suggesting that its biological function may not be just glycoside hydrolysis. Cellobiohydrolase I hydrolyses only beta-cellobiosyl fluoride, which is, however, an approx. 1-3% contaminant in alpha-cellobiosyl fluoride as prepared and purified by conventional methods. Instrumental assays for the various components of the cellulase complex are discussed.

Limited hydrolysis of β-casein by cell wall proteinase and its effect on hydrolysates's conformational and structural properties

2014 ◽  
Vol 50 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Xiaofen Ren ◽  
Daodong Pan ◽  
Zhen Wu ◽  
Xiaoqun Zeng ◽  
Yangying Sun ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Properties ◽  
Hydrolysis Of

Structural properties and enzymatic hydrolysis of rice straw

2006 ◽  
Vol 41 (6) ◽  
pp. 1261-1264 ◽  
Author(s):  
Shengying Jin ◽  
Hongzhang Chen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Properties ◽  
Rice Straw ◽  
Hydrolysis Of
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