scholarly journals Natural Killer Cells-Produced IFN-γ Improves Bone Marrow-Derived Hepatocytes Regeneration in Murine Liver Failure Model

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Lu Li ◽  
Zhutian Zeng ◽  
Ziping Qi ◽  
Xin Wang ◽  
Xiang Gao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Natural Killer Cells ◽  
Liver Failure ◽  
Natural Killer ◽  
Failure Model ◽  
Killer Cells ◽  
Murine Liver ◽  
Ifn Γ

Increased production of IFN-γ by natural killer cells triggered with bone marrow-derived dendritic cells cultured in the presence of retinoic acid

2013 ◽  
Vol 715 (1-3) ◽  
pp. 321-327 ◽  
Author(s):  
Jessie Chau ◽  
Dasha Moza ◽  
Nazia Hossain ◽  
Jeffrey K. Lee ◽  
John Bienenstock ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Retinoic Acid ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Killer Cells ◽  
Ifn Γ ◽  
Cells Cultured

Higher circulating natural killer cells and lower lactate levels at admission predict spontaneous survival in non-acetaminophen induced acute liver failure

2021 ◽  
Vol 231 ◽  
pp. 108829
Author(s):  
Tanvi Agrawal ◽  
Rakhi Maiwall ◽  
V. Rajan ◽  
Meenu Bajpai ◽  
Rakesh Kumar Jagdish ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Natural Killer ◽  
Killer Cells ◽  
Lactate Levels

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

2017 ◽  
Vol 153 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Baifen Song ◽  
Xiaoting Li ◽  
Jinzhu Ma ◽  
Liquan Yu ◽  
Zhenyue Feng ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Prokaryotic Expression ◽  
Interferon Γ ◽  
Fibroblast Cells ◽  
Killer Cells ◽  
Chicken Embryonic Fibroblast ◽  
Prokaryotic Expression Vector ◽  
Ifn Γ ◽  
Embryonic Fibroblast

Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified. To determine their antiviral activities, 200 ng/mL of ChIL-18 and/or ChIFN-γ were inoculated into chicken embryonic fibroblast cells. After 24 h, one 50% tissue culture infective dose (TCID50) of infectious bursal disease virus (IBDV) was inoculated into the chicken embryonic fibroblast cells. The results showed that the antiviral effect of ChIL-18 and ChIFN-γ in combination was better than that of ChIL-18 or ChIFN-γ alone. Next, 14-day-old chicken were injected with 200 µg of ChIL-18 and/or ChIFN-γ and then were challenged with 103 TCID50 of IBDV via intraperitoneal injection. The results showed that the proliferation of IBDV was inhibited by the injection of the recombinant proteins, especially the combination of ChIL-18 and ChIFN-γ, as evidenced by cytokine detection, quantitative PCR, and pathology analyses. These results indicate that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL-18 and ChIFN-γ has a better inhibitory effect than either cytokine alone.

Characterization of a subset of bone marrow-derived natural killer cells that regulates T cell activation in rats

2008 ◽  
Vol 83 (5) ◽  
pp. 1128-1135 ◽  
Author(s):  
Taba Kheradmand ◽  
Prachi P. Trivedi ◽  
Norbert A. Wolf ◽  
Paul C. Roberts ◽  
Robert H. Swanborg
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
T Cell Activation ◽  
Cell Activation ◽  
Killer Cells
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