scholarly journals The immune adherence receptor CR1-like existed on porcine erythrocytes membrane

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Wei Yin ◽  
Jiaoyan Cui ◽  
Junbing Jiang ◽  
Junxing Zhao ◽  
Kuohai Fan ◽  
...  
Keyword(s):  
Immune Adherence

scholarly journals Immune Adherence–mediated Opsonophagocytosis:  The Mechanism of Leishmania Infection

1999 ◽  
Vol 189 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Mercedes Domínguez ◽  
Alfredo Toraño
Keyword(s):  
Ex Vivo ◽  
Leishmania Infection ◽  
Ex Vivo Model ◽  
Blood Leukocytes ◽  
Classical Complement Pathway ◽  
Host Interaction ◽  
Immune Adherence ◽  
Intracellular Parasites ◽  
Adherence Mechanism ◽  
Leishmania Promastigotes

To mimic the sandfly pool feeding process and characterize the cellular and biochemical events that occur during the early stages of promastigote–host interaction, we developed an ex vivo model of human blood infection with Leishmania promastigotes. Within 30 s of blood contact, Leishmania promastigotes bind natural anti–Leishmania antibodies, which then activate the classical complement pathway and opsonization by the third component of complement. The opsonized promastigotes undergo an immune adherence reaction and bind quantitatively to erythrocyte CR1 receptors; opsonized Leishmania amastigotes also bind to erythrocytes. Progression of infection implies promastigote transfer from erythrocytes to acceptor blood leukocytes. After 10 min of ex vivo infection, 25% of all leukocytes contain intracellular parasites, indicating that blood cells are the early targets for the invading promastigotes. We propose that adaptation to the immune adherence mechanism aids Leishmania survival, promoting rapid promastigote phagocytosis by leukocytes. This facilitates host colonization and may represent the parasite's earliest survival strategy. In light of this mechanism, it is unlikely that infection-blocking vaccines can be developed.

scholarly journals Utility of Immune Adherence Hemagglutination Method for Antibody Measurement of Varicella Vaccination

2012 ◽  
Vol 86 (2) ◽  
pp. 146-148 ◽  
Author(s):  
Takao OZAKI ◽  
Naoko NISHIMURA ◽  
Kensei GOTOH ◽  
Shinji KAWABE ◽  
Keiji FUNAHASHI ◽  
...  
Keyword(s):  
Varicella Vaccination ◽  
Immune Adherence

Promoting adaptive immunity: A new aspect to Immune Adherence?

Immunobiology ◽  
2016 ◽  
Vol 221 (10) ◽  
pp. 1164
Author(s):  
Steven P. Broadley ◽  
Ann Plaumann ◽  
Raffaele Coletti ◽  
Christin Lehmann ◽  
Steffen Massberg ◽  
...  
Keyword(s):  
Adaptive Immunity ◽  
Immune Adherence

scholarly journals Polymorphic glycoprotein-1 on mouse platelets: possible role of Pgp-1 and LFA-1 in antibody-dependent platelet cytotoxicity involving complement

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 211-218
Author(s):  
PJ McCaffery ◽  
AS Tan ◽  
MV Berridge
Keyword(s):  
Cell Surface ◽  
Peripheral Blood ◽  
Broad Band ◽  
Peritoneal Macrophages ◽  
Beta Subunits ◽  
Deficient Mouse ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Functional Studies ◽  
Immune Adherence

The presence of the Pgp-1 glycoprotein on mouse platelets is demonstrated by antibody-binding techniques, by immunoprecipitation, and by transblotting using the monoclonal antibody (MoAb) C71/26 against Pgp-1. C71/26 immunoprecipitates as a broad band of mol wt 87,000 to 100,000 as determined by radioiodination of the platelet cell surface and by the 3H-sodium borohydride labeling technique. Immunoblotting showed Pgp-1 expression on platelets to be quantitatively similar to its presence on macrophages and resolved platelet Pgp-1 into two bands of mol wt 87,000 and 97,000 whereas Pgp-1 on parasite-elicited peritoneal macrophages showed 82,000 and 87,000 mol wt species. Platelets and monocyte/macrophage cells from either peripheral blood or from the peritoneal cavity showed homogeneous binding of Pgp-1 antibody to greater than 97% of cells by flow cytometry. In contrast, lymphocytes from peripheral blood or from the spleen showed a heterogeneous binding pattern with 20% to 30% of cells being negative, and the majority weakly positive. In functional studies, MoAbs against CR1 and CR3 substantially inhibited platelet immune adherence, whereas C71/26 showed only marginal inhibitor. In contrast, C71/26 and other MoAbs against Pgp-1 inhibited platelet- dependent cytotoxicity of antibody-coated sheep erythrocytes in the presence of C5-deficient mouse plasma whereas M1/70 against CR3 showed no effect. In this assay, MoAbs against the alpha- and beta-subunits of leukocyte functional molecule LFA-1 also inhibited platelet cytotoxicity. These results show that the platelet cell surface moieties Pgp-1 and LFA-1 are involved in or closely associated with antibody-dependent cellular cytotoxicity by platelets.

scholarly journals Endotoxin-induced platelet aggregation and secretion. I. Morphological changes and pharmacological effects

1977 ◽  
Vol 28 (1) ◽  
pp. 211-223
Author(s):  
D.E. MacIntyre ◽  
A.P. Allen ◽  
K.J. Thorne ◽  
A.M. Glauert ◽  
J.L. Gordon
Keyword(s):  
Platelet Aggregation ◽  
Prostaglandin E1 ◽  
Alkylating Agent ◽  
Morphological Changes ◽  
Blood Platelets ◽  
Second Phase ◽  
Pharmacological Effects ◽  
Prostaglandin Biosynthesis ◽  
Immune Adherence ◽  
Induced Aggregation

Endotoxin lipopolysaccharide (LPS) from Acinetobacter 199A induced aggregation of blood platelets from immune adherence-positive species (rat, rabbit) but not from immune adherence-negative species such as pig and man. Aggregation occurred in 2 phases: the first was not accompanied by secretion of platelet constituents, was apparently a consequence of C3 activation, and was selectively inhibited by EGTA. The second phase of aggregation was associated with secretion of platelet granule contents, and with a lesser amount of cytoplasmic leakage. Secondary aggregation was abolished by the sulphydryl alkylating agent N-ethylmaleimide, and by agents which increased the level of cyclic AMP in platelets, such as prostaglandin E1 (a stimulator of adenylate cyclase) and methyl xanthines (inhibitors of phosphodiesterase). Secondary aggregation was partly inhibited by agents which block platelet prostaglandin biosynthesis (e.g. aspirin, indomethacin). Primary aggregation was unaffected by these inhibitors at concentrations which blocked secondary aggregation.

Negative Serology for Hepatitis A and B Viruses in 18 Cases of Neonatal Cholestasis

PEDIATRICS ◽  
1980 ◽  
Vol 66 (2) ◽  
pp. 269-271
Author(s):  
William F. Balistreri ◽  
Edward Tabor ◽  
Robert J. Gerety
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Maternal Serum ◽  
Hbv Infection ◽  
Core Antigen ◽  
Neonatal Cholestasis ◽  
Immune Adherence ◽  
B Virus ◽  
Serologic Evidence

Serologic evidence of hepatitis A virus (HAV) or hepatitis B virus (HBV) infection was sought in 14 patients with biliary atresia and in four patients with neonatal hepatitis; maternal serum was also analyzed. Specific sensitive radioimmunoassays were used to detect HBV surface antigen (HBsAg) and antibody (anti-HBs); complement fixation was used to detect antibody to HBV core antigen (anti-HBc). Antibody to HAV (anti-HAV) was assayed by radioimmunoassay, as well as by immune adherence hemagglutination. There was no evidence of active or past HBV infection in any infant or mother studied. All three infants with detectable anti-HAV were born to mothers similarly anti-HAV positive; serial testing of sera from two of these infants documented disappearance of detectable anti-HAV by 9 months of age. It is unlikely, therefore, that either HAV or HBV had an etiologic role in neonatal cholestasis in these patients. The role of other (non-A, non-B) hepatitis viruses or nonviral etiologies must be investigated.

Detection of Australia Antigen by Means of Immune Adherence Haemagglutination Test

Vox Sanguinis ◽  
1971 ◽  
Vol 20 (2) ◽  
pp. 178-181 ◽  
Author(s):  
M. Mayumi ◽  
K. Okochi ◽  
K. Nishioka
Keyword(s):  
Australia Antigen ◽  
Haemagglutination Test ◽  
Immune Adherence

Immune Adherence (IA), Sarles Phenomenon (SP) and Diffusion Chamber Method (DC)

1990 ◽  
pp. 173-181 ◽  
Author(s):  
H. Ishikura ◽  
Y. Kikuchi ◽  
O. Toyokawa ◽  
H. Hayasaka ◽  
K. Kikuchi
Keyword(s):  
Diffusion Chamber ◽  
Chamber Method ◽  
Immune Adherence ◽  
And Diffusion
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