scholarly journals Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Lianhua Dong ◽  
Ying Meng ◽  
Zhiwei Sui ◽  
Jing Wang ◽  
Liqing Wu ◽  
...  
Keyword(s):  
Reference Material ◽  
Plasmid Dna ◽  
Copy Number ◽  
Digital Pcr ◽  
Dna Copy Number ◽  
Accurate Quantification

scholarly journals Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material

2016 ◽  
Vol 9 ◽  
pp. 29-39 ◽  
Author(s):  
Liesbet Deprez ◽  
Philippe Corbisier ◽  
Anne-Marie Kortekaas ◽  
Stéphane Mazoua ◽  
Roxana Beaz Hidalgo ◽  
...  
Keyword(s):  
Reference Material ◽  
Certified Reference Material ◽  
Copy Number ◽  
Digital Pcr ◽  
Dna Copy Number ◽  
Pcr Method

scholarly journals Development and interlaboratory evaluation of a NIST Reference Material RM 8366 for EGFR and MET gene copy number measurements

2019 ◽  
Vol 57 (8) ◽  
pp. 1142-1152 ◽  
Author(s):  
Hua-Jun He ◽  
Biswajit Das ◽  
Megan H. Cleveland ◽  
Li Chen ◽  
Corinne E. Camalier ◽  
...  
Keyword(s):  
Reference Material ◽  
Copy Number ◽  
Target Genes ◽  
Human Cancer ◽  
Gene Copy Number ◽  
Digital Pcr ◽  
Cancer Diagnostics ◽  
Gene Copy ◽  
Human Cancer Cell Lines ◽  
Copy Numbers

Abstract Background The National Institute of Standards and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR (epidermal growth factor receptor) and MET (proto-oncogene, receptor tyrosine kinase), important targets for cancer diagnostics and treatment. The reference material is composed of genomic DNA prepared from six human cancer cell lines with different levels of amplification of the target genes. Methods The reference values for the ratios of the EGFR and MET gene copy numbers to the copy numbers of reference genes were measured using digital PCR. The digital PCR measurements were confirmed by two additional laboratories. The samples were also characterized using Next Generation Sequencing (NGS) methods including whole genome sequencing (WGS) at three levels of coverage (approximately 1 ×, 5 ×  and greater than 30 ×), whole exome sequencing (WES), and two different pan-cancer gene panels. The WES data were analyzed using three different bioinformatic algorithms. Results The certified values (digital PCR) for EGFR and MET were in good agreement (within 20%) with the values obtained from the different NGS methods and algorithms for five of the six components; one component had lower NGS values. Conclusions This study shows that NIST RM 8366 is a valuable reference material to evaluate the performance of assays that assess EGFR and MET gene copy number measurements.

scholarly journals High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number

2011 ◽  
Vol 83 (22) ◽  
pp. 8604-8610 ◽  
Author(s):  
Benjamin J. Hindson ◽  
Kevin D. Ness ◽  
Donald A. Masquelier ◽  
Phillip Belgrader ◽  
Nicholas J. Heredia ◽  
...  
Keyword(s):  
High Throughput ◽  
Copy Number ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Dna Copy Number ◽  
Absolute Quantitation

scholarly journals Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution

2019 ◽  
Vol 29 (11) ◽  
pp. 1878-1888 ◽  
Author(s):  
Ryan O'Hara ◽  
Enzo Tedone ◽  
Andrew Ludlow ◽  
Ejun Huang ◽  
Beatrice Arosio ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Single Cell ◽  
Copy Number ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Dna Copy Number ◽  
Copy Number Determination ◽  
Mitochondrial Dna Copy Number

scholarly journals Accurate quantification of T cells in copy number stable and unstable DNA samples using multiplex digital PCR

2021 ◽  
Author(s):  
Rogier Jacobus Nell ◽  
Willem Hendrik Zoutman ◽  
Neus Calbet-Llopart ◽  
Adriana Patricia Garcia ◽  
Nino Valentijn Menger ◽  
...  
Keyword(s):  
T Cells ◽  
Copy Number ◽  
Digital Pcr ◽  
Accurate Quantification
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